Clinical use and reporting of neurofilament quantification in neurological disorders: A global overview

Constance Delaby; Aurélie Ladang; Jose Martinez-Yriarte; Chiara Zecca; Giancarlo Logroscino; Peter Körtvelyessy; Hayrettin Tumani; Piero Parchi; Isabelle Quadrio; Melanie Hart; Dorte Aalund Olsen; Daniel Alcolea; Kaj Blennow; Juan Fortea; Alberto Lleo; Alicia Algeciras-Schimnich; Xavier Ayrignac; Aurélie Bedel; Gustavo A.A. Santos; Wyllians Borelli; Elodie Bouaziz-Amar; Inês Baldeiras; Edith Bigot-Corbel; Maria Bjerke; Mercedes Carretero Perez; Tiziana Casoli; Tinatin Chabrashvili; Miles D. Chapman; Jessica Cusato; Erdinc Dursun; Anthony Fourier; Daniela Galimberti; Duygu Gezen-Ak; Brian A. Gordon; Julien Gouju; Silvia de las Heras Florez; Juanjo Hernandez Sanchez; Marina Herwerth; Daniele Imperiale; Flora Kaczorowski; Kensaku Kasuga; Ashvini Keshavan; Michael Khalil; Jens Kuhle; Christoph Leithner; Piotr Lewczuk; Franck Letournel; Magda Tsolaki; Guido Maria Giuffrè; Marie Céline Blanc; Barbara Mroczko; Jose Enrique Martínez Rodríguez; Giulia Musso; Agnieszka Kulczynska-Przybik; Léonor Nogueira; Claire Paquet; Simone Baiardi; Lorenzo Gaetani; Lucilla Parnetti; Raquel Perez Garay; Koen Poesen; Muriel Quillard-Muraine; Enrique Rodriguez Borja; Susanna Schraen; Daniela Terracciano; Franziska Bachhuber; Steffen Halbgebauer; Socrates Tzartos; John Tzartos; Nadine Unterwalder; Lisa Vermunt; Cheryl L. Wellington; Henrik Zetterberg; Charlotte Teunissen; Sylvain Lehmann

doi:10.1002/alz.70343

Clinical use and reporting of neurofilament quantification in neurological disorders: A global overview

Constance Delaby
, Aurélie Ladang
, Jose Martinez-Yriarte
, Chiara Zecca
, Giancarlo Logroscino
, Peter Körtvelyessy
, Hayrettin Tumani
, Piero Parchi
, Isabelle Quadrio
, Melanie Hart
, Dorte Aalund Olsen
, Daniel Alcolea
, Kaj Blennow
, Juan Fortea
, Alberto Lleo
, Alicia Algeciras-Schimnich
, Xavier Ayrignac
, Aurélie Bedel
, Gustavo A.A. Santos
, Wyllians Borelli

Elodie Bouaziz-Amar, Inês Baldeiras, Edith Bigot-Corbel, Maria Bjerke, Mercedes Carretero Perez, Tiziana Casoli, Tinatin Chabrashvili, Miles D. Chapman, Jessica Cusato, Erdinc Dursun, Anthony Fourier, Daniela Galimberti, Duygu Gezen-Ak, Brian A. Gordon, Julien Gouju, Silvia de las Heras Florez, Juanjo Hernandez Sanchez, Marina Herwerth, Daniele Imperiale, Flora Kaczorowski, Kensaku Kasuga, Ashvini Keshavan, Michael Khalil, Jens Kuhle, Christoph Leithner, Piotr Lewczuk, Franck Letournel, Magda Tsolaki, Guido Maria Giuffrè, Marie Céline Blanc, Barbara Mroczko, Jose Enrique Martínez Rodríguez, Giulia Musso, Agnieszka Kulczynska-Przybik, Léonor Nogueira, Claire Paquet, Simone Baiardi, Lorenzo Gaetani, Lucilla Parnetti, Raquel Perez Garay, Koen Poesen, Muriel Quillard-Muraine, Enrique Rodriguez Borja, Susanna Schraen, Daniela Terracciano, Franziska Bachhuber, Steffen Halbgebauer, Socrates Tzartos, John Tzartos, Nadine Unterwalder, Lisa Vermunt, Cheryl L. Wellington, Henrik Zetterberg, Charlotte Teunissen, Sylvain Lehmann^*

^*Kontaktforfatter

KI, OUH, Forskningsenhed for Klinisk biokemi (Odense)

Université de Montpellier – Université Paul Valéry – EPHE
Hospital de la Santa Creu i Sant Pau
University Hospital of Liège
Hospital do Meixoeiro
University of Bari Aldo Moro
Charité - University Medicine Berlin
Deutsches Institut für Neurodegenerative Erkrankungen (DZNE) Site Munich
Division Innovation
University of Ulm
University of Bologna
The Institute of Neurological Sciences of Bologna
Hospices Civils de Lyon/Centre Léon Bérard
UCL Queen Square Institute of Neurology
CIBERNED
University of Gothenburg
Mayo Clinic
University Hospital Center
University of São Paulo
São Leopoldo MANDIC School of Medicine
University of Campinas
Universidade Federal do Rio Grande do Sul
Assistance Publique Hôpitaux de Paris
Coimbra University
University Hospital of Nantes
University Hospital Brussels
Santa Cruz de Tenerife
Italian National Research Center on Aging (INRCA)
State University of New York Upstate Medical University
University College London Hospitals NHS Foundation Trust
UNITO
Istanbul University-Cerrahpaşa
University of Milan
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
Washington University in St. Louis
Angers University Hospital
Universidad de La Laguna
Laboratori de Referència de Catalunya
University Hospital Zurich
Brain Research Institute
Medical University of Graz
University Hospital of Basel
Universitätsklinikum Erlagen
Medical University of Bialystok
AHEPA University Hospital
Catholic University of the Sacred Heart
Hospital Cochin
Hospital del Mar
University of Padova
Service de Génétique Médicale
Paris Hospitals and Paris Descartes University
University of Perugia
Hospital de Cruces
Leuven Brain Institute
Rouen University Hospital
University of Valencia
Lille University Hospital
University of Naples Federico II
Tzartos NeuroDiagnostics
National and Kapodistrian University of Athens
Labor Berlin
Vrije University Amsterdam
The University of British Columbia

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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Abstract

INTRODUCTION: Neurofilament light chain (NfL) quantification aids in diagnosing and predicting neurological disorders, but clinical and laboratory practices vary across centers. Differences in result interpretation and reporting further challenge test commutability. This study aimed to review the global analytical and post-analytical methods used for NfL measurement in routine clinical practice across different contexts. METHODS: We established an international working group (WG) and distributed a survey to its members to gather information on context of use (COU), (pre) analytical methods, cutoff usage, as well as the interpretation and reporting of NfL measurements. RESULTS: Among the centers, 63% measured NfL in cerebrospinal fluid (CSF), 87% in blood, and 53% in both. COU was widespread, with 50% defining pathological cutoffs based on publications and 42% considering age. Reporting was primarily done through numeric results (95%). DISCUSSION: Harmonizing cutoffs, reporting, and interpretation across various clinical contexts will facilitate the incorporation of this biomarker into routine clinical practice. Highlights: Unique international overview of current analytical and post-analytical methods for neurofilament light chain (NfL) measurement in routine clinical practice. Tailored sheets for each neurological application. Strategies to harmonize cutoffs, reporting, and interpretation of NfL's measurement.

Originalsprog	Engelsk
Artikelnummer	e70343
Tidsskrift	Alzheimer's and Dementia
Vol/bind	21
Udgave nummer	6
Antal sider	16
ISSN	1552-5260
DOI	https://doi.org/10.1002/alz.70343
Status	Udgivet - jun. 2025

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10.1002/alz.70343Licens: CC BY-NC-ND

Open Access VersionForlagets udgivne version, 0,98 MBLicens: CC BY-NC-ND

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Citationsformater

Delaby, C., Ladang, A., Martinez-Yriarte, J., Zecca, C., Logroscino, G., Körtvelyessy, P., Tumani, H., Parchi, P., Quadrio, I., Hart, M., Olsen, D. A., Alcolea, D., Blennow, K., Fortea, J., Lleo, A., Algeciras-Schimnich, A., Ayrignac, X., Bedel, A., Santos, G. A. A., ... Lehmann, S. (2025). Clinical use and reporting of neurofilament quantification in neurological disorders: A global overview. Alzheimer's and Dementia, 21(6), Artikel e70343. https://doi.org/10.1002/alz.70343

@article{5da67b3b37734e0084b8cce8ed031b1c,

title = "Clinical use and reporting of neurofilament quantification in neurological disorders: A global overview",

abstract = "INTRODUCTION: Neurofilament light chain (NfL) quantification aids in diagnosing and predicting neurological disorders, but clinical and laboratory practices vary across centers. Differences in result interpretation and reporting further challenge test commutability. This study aimed to review the global analytical and post-analytical methods used for NfL measurement in routine clinical practice across different contexts. METHODS: We established an international working group (WG) and distributed a survey to its members to gather information on context of use (COU), (pre) analytical methods, cutoff usage, as well as the interpretation and reporting of NfL measurements. RESULTS: Among the centers, 63\% measured NfL in cerebrospinal fluid (CSF), 87\% in blood, and 53\% in both. COU was widespread, with 50\% defining pathological cutoffs based on publications and 42\% considering age. Reporting was primarily done through numeric results (95\%). DISCUSSION: Harmonizing cutoffs, reporting, and interpretation across various clinical contexts will facilitate the incorporation of this biomarker into routine clinical practice. Highlights: Unique international overview of current analytical and post-analytical methods for neurofilament light chain (NfL) measurement in routine clinical practice. Tailored sheets for each neurological application. Strategies to harmonize cutoffs, reporting, and interpretation of NfL's measurement.",

keywords = "biomarkers, blood, cerebrospinal fluid, clinical report, consensus approach, harmonization, neurofilaments",

author = "Constance Delaby and Aur{\'e}lie Ladang and Jose Martinez-Yriarte and Chiara Zecca and Giancarlo Logroscino and Peter K{\"o}rtvelyessy and Hayrettin Tumani and Piero Parchi and Isabelle Quadrio and Melanie Hart and Olsen, \{Dorte Aalund\} and Daniel Alcolea and Kaj Blennow and Juan Fortea and Alberto Lleo and Alicia Algeciras-Schimnich and Xavier Ayrignac and Aur{\'e}lie Bedel and Santos, \{Gustavo A.A.\} and Wyllians Borelli and Elodie Bouaziz-Amar and In{\^e}s Baldeiras and Edith Bigot-Corbel and Maria Bjerke and \{Carretero Perez\}, Mercedes and Tiziana Casoli and Tinatin Chabrashvili and Chapman, \{Miles D.\} and Jessica Cusato and Erdinc Dursun and Anthony Fourier and Daniela Galimberti and Duygu Gezen-Ak and Gordon, \{Brian A.\} and Julien Gouju and \{de las Heras Florez\}, Silvia and \{Hernandez Sanchez\}, Juanjo and Marina Herwerth and Daniele Imperiale and Flora Kaczorowski and Kensaku Kasuga and Ashvini Keshavan and Michael Khalil and Jens Kuhle and Christoph Leithner and Piotr Lewczuk and Franck Letournel and Magda Tsolaki and Giuffr{\`e}, \{Guido Maria\} and Blanc, \{Marie C{\'e}line\} and Barbara Mroczko and \{Mart{\'i}nez Rodr{\'i}guez\}, \{Jose Enrique\} and Giulia Musso and Agnieszka Kulczynska-Przybik and L{\'e}onor Nogueira and Claire Paquet and Simone Baiardi and Lorenzo Gaetani and Lucilla Parnetti and \{Perez Garay\}, Raquel and Koen Poesen and Muriel Quillard-Muraine and \{Rodriguez Borja\}, Enrique and Susanna Schraen and Daniela Terracciano and Franziska Bachhuber and Steffen Halbgebauer and Socrates Tzartos and John Tzartos and Nadine Unterwalder and Lisa Vermunt and Wellington, \{Cheryl L.\} and Henrik Zetterberg and Charlotte Teunissen and Sylvain Lehmann",

year = "2025",

month = jun,

doi = "10.1002/alz.70343",

language = "English",

volume = "21",

journal = "Alzheimer's and Dementia",

issn = "1552-5260",

publisher = "Wiley",

number = "6",

}

Delaby, C, Ladang, A, Martinez-Yriarte, J, Zecca, C, Logroscino, G, Körtvelyessy, P, Tumani, H, Parchi, P, Quadrio, I, Hart, M, Olsen, DA, Alcolea, D, Blennow, K, Fortea, J, Lleo, A, Algeciras-Schimnich, A, Ayrignac, X, Bedel, A, Santos, GAA, Borelli, W, Bouaziz-Amar, E, Baldeiras, I, Bigot-Corbel, E, Bjerke, M, Carretero Perez, M, Casoli, T, Chabrashvili, T, Chapman, MD, Cusato, J, Dursun, E, Fourier, A, Galimberti, D, Gezen-Ak, D, Gordon, BA, Gouju, J, de las Heras Florez, S, Hernandez Sanchez, J, Herwerth, M, Imperiale, D, Kaczorowski, F, Kasuga, K, Keshavan, A, Khalil, M, Kuhle, J, Leithner, C, Lewczuk, P, Letournel, F, Tsolaki, M, Giuffrè, GM, Blanc, MC, Mroczko, B, Martínez Rodríguez, JE, Musso, G, Kulczynska-Przybik, A, Nogueira, L, Paquet, C, Baiardi, S, Gaetani, L, Parnetti, L, Perez Garay, R, Poesen, K, Quillard-Muraine, M, Rodriguez Borja, E, Schraen, S, Terracciano, D, Bachhuber, F, Halbgebauer, S, Tzartos, S, Tzartos, J, Unterwalder, N, Vermunt, L, Wellington, CL, Zetterberg, H, Teunissen, C & Lehmann, S 2025, 'Clinical use and reporting of neurofilament quantification in neurological disorders: A global overview', Alzheimer's and Dementia, bind 21, nr. 6, e70343. https://doi.org/10.1002/alz.70343

TY - JOUR

T1 - Clinical use and reporting of neurofilament quantification in neurological disorders

T2 - A global overview

AU - Delaby, Constance

AU - Ladang, Aurélie

AU - Martinez-Yriarte, Jose

AU - Zecca, Chiara

AU - Logroscino, Giancarlo

AU - Körtvelyessy, Peter

AU - Tumani, Hayrettin

AU - Parchi, Piero

AU - Quadrio, Isabelle

AU - Hart, Melanie

AU - Olsen, Dorte Aalund

AU - Alcolea, Daniel

AU - Blennow, Kaj

AU - Fortea, Juan

AU - Lleo, Alberto

AU - Algeciras-Schimnich, Alicia

AU - Ayrignac, Xavier

AU - Bedel, Aurélie

AU - Santos, Gustavo A.A.

AU - Borelli, Wyllians

AU - Bouaziz-Amar, Elodie

AU - Baldeiras, Inês

AU - Bigot-Corbel, Edith

AU - Bjerke, Maria

AU - Carretero Perez, Mercedes

AU - Casoli, Tiziana

AU - Chabrashvili, Tinatin

AU - Chapman, Miles D.

AU - Cusato, Jessica

AU - Dursun, Erdinc

AU - Fourier, Anthony

AU - Galimberti, Daniela

AU - Gezen-Ak, Duygu

AU - Gordon, Brian A.

AU - Gouju, Julien

AU - de las Heras Florez, Silvia

AU - Hernandez Sanchez, Juanjo

AU - Herwerth, Marina

AU - Imperiale, Daniele

AU - Kaczorowski, Flora

AU - Kasuga, Kensaku

AU - Keshavan, Ashvini

AU - Khalil, Michael

AU - Kuhle, Jens

AU - Leithner, Christoph

AU - Lewczuk, Piotr

AU - Letournel, Franck

AU - Tsolaki, Magda

AU - Giuffrè, Guido Maria

AU - Blanc, Marie Céline

AU - Mroczko, Barbara

AU - Martínez Rodríguez, Jose Enrique

AU - Musso, Giulia

AU - Kulczynska-Przybik, Agnieszka

AU - Nogueira, Léonor

AU - Paquet, Claire

AU - Baiardi, Simone

AU - Gaetani, Lorenzo

AU - Parnetti, Lucilla

AU - Perez Garay, Raquel

AU - Poesen, Koen

AU - Quillard-Muraine, Muriel

AU - Rodriguez Borja, Enrique

AU - Schraen, Susanna

AU - Terracciano, Daniela

AU - Bachhuber, Franziska

AU - Halbgebauer, Steffen

AU - Tzartos, Socrates

AU - Tzartos, John

AU - Unterwalder, Nadine

AU - Vermunt, Lisa

AU - Wellington, Cheryl L.

AU - Zetterberg, Henrik

AU - Teunissen, Charlotte

AU - Lehmann, Sylvain

PY - 2025/6

Y1 - 2025/6

N2 - INTRODUCTION: Neurofilament light chain (NfL) quantification aids in diagnosing and predicting neurological disorders, but clinical and laboratory practices vary across centers. Differences in result interpretation and reporting further challenge test commutability. This study aimed to review the global analytical and post-analytical methods used for NfL measurement in routine clinical practice across different contexts. METHODS: We established an international working group (WG) and distributed a survey to its members to gather information on context of use (COU), (pre) analytical methods, cutoff usage, as well as the interpretation and reporting of NfL measurements. RESULTS: Among the centers, 63% measured NfL in cerebrospinal fluid (CSF), 87% in blood, and 53% in both. COU was widespread, with 50% defining pathological cutoffs based on publications and 42% considering age. Reporting was primarily done through numeric results (95%). DISCUSSION: Harmonizing cutoffs, reporting, and interpretation across various clinical contexts will facilitate the incorporation of this biomarker into routine clinical practice. Highlights: Unique international overview of current analytical and post-analytical methods for neurofilament light chain (NfL) measurement in routine clinical practice. Tailored sheets for each neurological application. Strategies to harmonize cutoffs, reporting, and interpretation of NfL's measurement.

AB - INTRODUCTION: Neurofilament light chain (NfL) quantification aids in diagnosing and predicting neurological disorders, but clinical and laboratory practices vary across centers. Differences in result interpretation and reporting further challenge test commutability. This study aimed to review the global analytical and post-analytical methods used for NfL measurement in routine clinical practice across different contexts. METHODS: We established an international working group (WG) and distributed a survey to its members to gather information on context of use (COU), (pre) analytical methods, cutoff usage, as well as the interpretation and reporting of NfL measurements. RESULTS: Among the centers, 63% measured NfL in cerebrospinal fluid (CSF), 87% in blood, and 53% in both. COU was widespread, with 50% defining pathological cutoffs based on publications and 42% considering age. Reporting was primarily done through numeric results (95%). DISCUSSION: Harmonizing cutoffs, reporting, and interpretation across various clinical contexts will facilitate the incorporation of this biomarker into routine clinical practice. Highlights: Unique international overview of current analytical and post-analytical methods for neurofilament light chain (NfL) measurement in routine clinical practice. Tailored sheets for each neurological application. Strategies to harmonize cutoffs, reporting, and interpretation of NfL's measurement.

KW - biomarkers

KW - blood

KW - cerebrospinal fluid

KW - clinical report

KW - consensus approach

KW - harmonization

KW - neurofilaments

U2 - 10.1002/alz.70343

DO - 10.1002/alz.70343

M3 - Journal article

C2 - 40551293

AN - SCOPUS:105009252668

SN - 1552-5260

VL - 21

JO - Alzheimer's and Dementia

JF - Alzheimer's and Dementia

IS - 6

M1 - e70343

ER -

Clinical use and reporting of neurofilament quantification in neurological disorders: A global overview

Abstract

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