Cleavage and protection of locked nucleic acid-modified DNA by restriction endonucleases

Lucile Crouzier, Camille Dubois, Jesper Wengel, Rakesh N Veedu

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Abstrakt

Locked nucleic acid (LNA) is one of the most prominent nucleic acid analogues reported so far. We herein for the first time report cleavage by restriction endonuclease of LNA-modified DNA oligonucleotides. The experiments revealed that RsaI is an efficient enzyme capable of recognizing and cleaving LNA-modified DNA oligonucleotides. Furthermore, introduction of LNA nucleotides protects against cleavage by the restriction endonucleases PvuII, PstI, SacI, KpnI and EcoRI.
OriginalsprogEngelsk
TidsskriftBioorganic & Medicinal Chemistry Letters
Vol/bind22
Udgave nummer14
Sider (fra-til)4836-4838
Antal sider3
ISSN0960-894X
DOI
StatusUdgivet - 2012

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