Locked nucleic acid (LNA) is one of the most prominent nucleic acid analogues reported so far. We herein for the first time report cleavage by restriction endonuclease of LNA-modified DNA oligonucleotides. The experiments revealed that RsaI is an efficient enzyme capable of recognizing and cleaving LNA-modified DNA oligonucleotides. Furthermore, introduction of LNA nucleotides protects against cleavage by the restriction endonucleases PvuII, PstI, SacI, KpnI and EcoRI.
Crouzier, L., Dubois, C., Wengel, J., & Veedu, R. N. (2012). Cleavage and protection of locked nucleic acid-modified DNA by restriction endonucleases. Bioorganic & Medicinal Chemistry Letters, 22(14), 4836-4838. https://doi.org/10.1016/j.bmcl.2012.05.113