Circulating microRNA expression profiles associated with systemic lupus erythematosus

Anting Liu Carlsen, Aaron J Schetter, Christoffer Nielsen, Christian Lood, Steen Knudsen, Anne Voss, Curtis C Harris, Thomas Hellmark, Mårten Segelmark, Søren Knak Jacobsen, Anders Bengtsson, Niels Henrik Helweg Heegaard

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

OBJECTIVE: To evaluate the specificity of expression patterns of cell-free, circulating microRNAs in systemic lupus erythematosus (SLE). METHODS: Total RNA was purified from plasma and 45 different specific mature microRNAs were determined using quantitative reverse transcription polymerase chain reaction assays. A total of 409 plasma samples from 364 different SLE patients, healthy controls, and controls with other autoimmune diseases were included. The results of the primary cohort of 62 SLE and 29 healthy controls were validated in two independent cohorts with a total of 69 SLE, 114 healthy controls, 38 vasculitis, 18 rheumatoid arthritis, and 20 immunosuppressed patients. RESULTS: Seven microRNAs were statistically significantly differentially expressed in SLE plasma. Increased expression was found for miR-142-3p and miR-181a while miR-106a, miR-17, miR-20a, miR-92a, and miR-203 were decreased. Also, miR-342-3p, miR-223, and miR-20a were significantly lower in SLE patients with active nephritis. A predictive model for SLE based on 2 or 4 microRNAs differentiated SLE from controls (76% accuracy) when validated independently (p
OriginalsprogEngelsk
TidsskriftExcerpta Medica. Section 31: Arthritis and Rheumatism
Vol/bind65
Udgave nummer5
Sider (fra-til)1324-1334
ISSN0014-4355
DOI
StatusUdgivet - 2013

Fingeraftryk

MicroRNAs
Nephritis
RNA
Polymerase Chain Reaction

Citer dette

Carlsen, A. L., Schetter, A. J., Nielsen, C., Lood, C., Knudsen, S., Voss, A., ... Heegaard, N. H. H. (2013). Circulating microRNA expression profiles associated with systemic lupus erythematosus. Excerpta Medica. Section 31: Arthritis and Rheumatism, 65(5), 1324-1334. https://doi.org/10.1002/art.37890
Carlsen, Anting Liu ; Schetter, Aaron J ; Nielsen, Christoffer ; Lood, Christian ; Knudsen, Steen ; Voss, Anne ; Harris, Curtis C ; Hellmark, Thomas ; Segelmark, Mårten ; Jacobsen, Søren Knak ; Bengtsson, Anders ; Heegaard, Niels Henrik Helweg. / Circulating microRNA expression profiles associated with systemic lupus erythematosus. I: Excerpta Medica. Section 31: Arthritis and Rheumatism. 2013 ; Bind 65, Nr. 5. s. 1324-1334.
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title = "Circulating microRNA expression profiles associated with systemic lupus erythematosus",
abstract = "OBJECTIVE: To evaluate the specificity of expression patterns of cell-free, circulating microRNAs in systemic lupus erythematosus (SLE). METHODS: Total RNA was purified from plasma and 45 different specific mature microRNAs were determined using quantitative reverse transcription polymerase chain reaction assays. A total of 409 plasma samples from 364 different SLE patients, healthy controls, and controls with other autoimmune diseases were included. The results of the primary cohort of 62 SLE and 29 healthy controls were validated in two independent cohorts with a total of 69 SLE, 114 healthy controls, 38 vasculitis, 18 rheumatoid arthritis, and 20 immunosuppressed patients. RESULTS: Seven microRNAs were statistically significantly differentially expressed in SLE plasma. Increased expression was found for miR-142-3p and miR-181a while miR-106a, miR-17, miR-20a, miR-92a, and miR-203 were decreased. Also, miR-342-3p, miR-223, and miR-20a were significantly lower in SLE patients with active nephritis. A predictive model for SLE based on 2 or 4 microRNAs differentiated SLE from controls (76{\%} accuracy) when validated independently (p",
author = "Carlsen, {Anting Liu} and Schetter, {Aaron J} and Christoffer Nielsen and Christian Lood and Steen Knudsen and Anne Voss and Harris, {Curtis C} and Thomas Hellmark and M{\aa}rten Segelmark and Jacobsen, {S{\o}ren Knak} and Anders Bengtsson and Heegaard, {Niels Henrik Helweg}",
note = "Copyright {\circledC} 2013 by the American College of Rheumatology.",
year = "2013",
doi = "10.1002/art.37890",
language = "English",
volume = "65",
pages = "1324--1334",
journal = "Excerpta Medica. Section 31: Arthritis and Rheumatism",
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Carlsen, AL, Schetter, AJ, Nielsen, C, Lood, C, Knudsen, S, Voss, A, Harris, CC, Hellmark, T, Segelmark, M, Jacobsen, SK, Bengtsson, A & Heegaard, NHH 2013, 'Circulating microRNA expression profiles associated with systemic lupus erythematosus', Excerpta Medica. Section 31: Arthritis and Rheumatism, bind 65, nr. 5, s. 1324-1334. https://doi.org/10.1002/art.37890

Circulating microRNA expression profiles associated with systemic lupus erythematosus. / Carlsen, Anting Liu; Schetter, Aaron J; Nielsen, Christoffer; Lood, Christian; Knudsen, Steen; Voss, Anne; Harris, Curtis C; Hellmark, Thomas; Segelmark, Mårten; Jacobsen, Søren Knak; Bengtsson, Anders; Heegaard, Niels Henrik Helweg.

I: Excerpta Medica. Section 31: Arthritis and Rheumatism, Bind 65, Nr. 5, 2013, s. 1324-1334.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Circulating microRNA expression profiles associated with systemic lupus erythematosus

AU - Carlsen, Anting Liu

AU - Schetter, Aaron J

AU - Nielsen, Christoffer

AU - Lood, Christian

AU - Knudsen, Steen

AU - Voss, Anne

AU - Harris, Curtis C

AU - Hellmark, Thomas

AU - Segelmark, Mårten

AU - Jacobsen, Søren Knak

AU - Bengtsson, Anders

AU - Heegaard, Niels Henrik Helweg

N1 - Copyright © 2013 by the American College of Rheumatology.

PY - 2013

Y1 - 2013

N2 - OBJECTIVE: To evaluate the specificity of expression patterns of cell-free, circulating microRNAs in systemic lupus erythematosus (SLE). METHODS: Total RNA was purified from plasma and 45 different specific mature microRNAs were determined using quantitative reverse transcription polymerase chain reaction assays. A total of 409 plasma samples from 364 different SLE patients, healthy controls, and controls with other autoimmune diseases were included. The results of the primary cohort of 62 SLE and 29 healthy controls were validated in two independent cohorts with a total of 69 SLE, 114 healthy controls, 38 vasculitis, 18 rheumatoid arthritis, and 20 immunosuppressed patients. RESULTS: Seven microRNAs were statistically significantly differentially expressed in SLE plasma. Increased expression was found for miR-142-3p and miR-181a while miR-106a, miR-17, miR-20a, miR-92a, and miR-203 were decreased. Also, miR-342-3p, miR-223, and miR-20a were significantly lower in SLE patients with active nephritis. A predictive model for SLE based on 2 or 4 microRNAs differentiated SLE from controls (76% accuracy) when validated independently (p

AB - OBJECTIVE: To evaluate the specificity of expression patterns of cell-free, circulating microRNAs in systemic lupus erythematosus (SLE). METHODS: Total RNA was purified from plasma and 45 different specific mature microRNAs were determined using quantitative reverse transcription polymerase chain reaction assays. A total of 409 plasma samples from 364 different SLE patients, healthy controls, and controls with other autoimmune diseases were included. The results of the primary cohort of 62 SLE and 29 healthy controls were validated in two independent cohorts with a total of 69 SLE, 114 healthy controls, 38 vasculitis, 18 rheumatoid arthritis, and 20 immunosuppressed patients. RESULTS: Seven microRNAs were statistically significantly differentially expressed in SLE plasma. Increased expression was found for miR-142-3p and miR-181a while miR-106a, miR-17, miR-20a, miR-92a, and miR-203 were decreased. Also, miR-342-3p, miR-223, and miR-20a were significantly lower in SLE patients with active nephritis. A predictive model for SLE based on 2 or 4 microRNAs differentiated SLE from controls (76% accuracy) when validated independently (p

U2 - 10.1002/art.37890

DO - 10.1002/art.37890

M3 - Journal article

VL - 65

SP - 1324

EP - 1334

JO - Excerpta Medica. Section 31: Arthritis and Rheumatism

JF - Excerpta Medica. Section 31: Arthritis and Rheumatism

SN - 0014-4355

IS - 5

ER -