Chromatin Immunoprecipitation for Identification of Protein-DNA Interactions in Human Cells

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Abstrakt

Chromatin immunoprecipitation (ChIP) is a powerful technique allowing for investigation of protein-DNA interactions in living cells. Here, we provide a detailed step-by-step protocol for ChIP and highlight important considerations, challenges and pitfalls often encountered in the ChIP procedure. Furthermore, we present data of key quality control (QC) steps and exemplify material performance validation on transcription factor ChIP to provide a QC guide for setting up ChIP. Finally, we provide guidelines for scaling of the ChIP procedure to ChIP sequencing (ChIP-seq) and discuss important considerations associated with this.

OriginalsprogEngelsk
TitelTwo-Hybrid Systems : Methods and Protocols
RedaktørerLuis Oñate-Sánchez
ForlagHumana Press
Publikationsdato2018
Sider335-352
ISBN (Trykt)978-1-4939-7870-0
ISBN (Elektronisk)978-1-4939-7871-7
DOI
StatusUdgivet - 2018
NavnMethods in Molecular Biology
Vol/bind1794
ISSN1064-3745

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