Cholesteatoma

a morphologic study of stratum corneum lipids

Viggo Svane-Knudsen, L. Halkierø-Sørensen, G. Rasmussen, P.D. Ottosen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Two different lipid-retaining fixation techniques permitted electron microscopic visualization of both intra- and extracellular lipids in cholesteatoma epithelium obtained from 25 patients. An increased number of intracellular lipid-containing Odland bodies was demonstrated, with a maximum in the basal layer of the stratum granulosum, while the superior layer contained substantially fewer organelles than are found in normal skin. At the stratum granulosum/stratum corneum interface lipids secreted from Odland bodies were found in sac-like invaginations along the cell membrane but premature exocytosis was also frequently observed. In the intercellular spaces of the stratum corneum, multiple long sheets of lamellar structures interrupted by slits or pores enclosed the keratinized corneocytes. The intercellular spaces seemed narrow and an extracellular barrier was not found until well above the stratum granulosum/stratum corneum interface. A similar distribution of Odland bodies and structure of intercellular lipids can occur in several dermatoses, where the formation and maintenance of the cutaneous permeability barrier are defective.

OriginalsprogEngelsk
TidsskriftActa Oto-Laryngologica
Vol/bind121
Udgave nummer5
Sider (fra-til)602-606
ISSN0001-6489
StatusUdgivet - 2001

Fingeraftryk

Cholesteatoma
Lipids
Skin
Exocytosis
Skin Diseases
Epithelium
Maintenance
Cell Membrane
Electrons

Citer dette

Svane-Knudsen, V., Halkierø-Sørensen, L., Rasmussen, G., & Ottosen, P. D. (2001). Cholesteatoma: a morphologic study of stratum corneum lipids. Acta Oto-Laryngologica, 121(5), 602-606.
Svane-Knudsen, Viggo ; Halkierø-Sørensen, L. ; Rasmussen, G. ; Ottosen, P.D. / Cholesteatoma : a morphologic study of stratum corneum lipids. I: Acta Oto-Laryngologica. 2001 ; Bind 121, Nr. 5. s. 602-606.
@article{05d26ed0ba9611dc9626000ea68e967b,
title = "Cholesteatoma: a morphologic study of stratum corneum lipids",
abstract = "Two different lipid-retaining fixation techniques permitted electron microscopic visualization of both intra- and extracellular lipids in cholesteatoma epithelium obtained from 25 patients. An increased number of intracellular lipid-containing Odland bodies was demonstrated, with a maximum in the basal layer of the stratum granulosum, while the superior layer contained substantially fewer organelles than are found in normal skin. At the stratum granulosum/stratum corneum interface lipids secreted from Odland bodies were found in sac-like invaginations along the cell membrane but premature exocytosis was also frequently observed. In the intercellular spaces of the stratum corneum, multiple long sheets of lamellar structures interrupted by slits or pores enclosed the keratinized corneocytes. The intercellular spaces seemed narrow and an extracellular barrier was not found until well above the stratum granulosum/stratum corneum interface. A similar distribution of Odland bodies and structure of intercellular lipids can occur in several dermatoses, where the formation and maintenance of the cutaneous permeability barrier are defective.",
author = "Viggo Svane-Knudsen and L. Halkier{\o}-S{\o}rensen and G. Rasmussen and P.D. Ottosen",
year = "2001",
language = "English",
volume = "121",
pages = "602--606",
journal = "Acta Oto-Laryngologica",
issn = "0001-6489",
publisher = "Taylor & Francis",
number = "5",

}

Svane-Knudsen, V, Halkierø-Sørensen, L, Rasmussen, G & Ottosen, PD 2001, 'Cholesteatoma: a morphologic study of stratum corneum lipids', Acta Oto-Laryngologica, bind 121, nr. 5, s. 602-606.

Cholesteatoma : a morphologic study of stratum corneum lipids. / Svane-Knudsen, Viggo; Halkierø-Sørensen, L.; Rasmussen, G.; Ottosen, P.D.

I: Acta Oto-Laryngologica, Bind 121, Nr. 5, 2001, s. 602-606.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Cholesteatoma

T2 - a morphologic study of stratum corneum lipids

AU - Svane-Knudsen, Viggo

AU - Halkierø-Sørensen, L.

AU - Rasmussen, G.

AU - Ottosen, P.D.

PY - 2001

Y1 - 2001

N2 - Two different lipid-retaining fixation techniques permitted electron microscopic visualization of both intra- and extracellular lipids in cholesteatoma epithelium obtained from 25 patients. An increased number of intracellular lipid-containing Odland bodies was demonstrated, with a maximum in the basal layer of the stratum granulosum, while the superior layer contained substantially fewer organelles than are found in normal skin. At the stratum granulosum/stratum corneum interface lipids secreted from Odland bodies were found in sac-like invaginations along the cell membrane but premature exocytosis was also frequently observed. In the intercellular spaces of the stratum corneum, multiple long sheets of lamellar structures interrupted by slits or pores enclosed the keratinized corneocytes. The intercellular spaces seemed narrow and an extracellular barrier was not found until well above the stratum granulosum/stratum corneum interface. A similar distribution of Odland bodies and structure of intercellular lipids can occur in several dermatoses, where the formation and maintenance of the cutaneous permeability barrier are defective.

AB - Two different lipid-retaining fixation techniques permitted electron microscopic visualization of both intra- and extracellular lipids in cholesteatoma epithelium obtained from 25 patients. An increased number of intracellular lipid-containing Odland bodies was demonstrated, with a maximum in the basal layer of the stratum granulosum, while the superior layer contained substantially fewer organelles than are found in normal skin. At the stratum granulosum/stratum corneum interface lipids secreted from Odland bodies were found in sac-like invaginations along the cell membrane but premature exocytosis was also frequently observed. In the intercellular spaces of the stratum corneum, multiple long sheets of lamellar structures interrupted by slits or pores enclosed the keratinized corneocytes. The intercellular spaces seemed narrow and an extracellular barrier was not found until well above the stratum granulosum/stratum corneum interface. A similar distribution of Odland bodies and structure of intercellular lipids can occur in several dermatoses, where the formation and maintenance of the cutaneous permeability barrier are defective.

M3 - Journal article

VL - 121

SP - 602

EP - 606

JO - Acta Oto-Laryngologica

JF - Acta Oto-Laryngologica

SN - 0001-6489

IS - 5

ER -

Svane-Knudsen V, Halkierø-Sørensen L, Rasmussen G, Ottosen PD. Cholesteatoma: a morphologic study of stratum corneum lipids. Acta Oto-Laryngologica. 2001;121(5):602-606.