Chemically modified oligonucleotides with efficient RNase H response

Birte Vester, Anne Marie Boel, Sune Lobedanz, B Ravindra Babu, Michael Raunkjaer, Dorthe Lindegaard, Raunak, Patrick J Hrdlicka, Torben Højland, Pawan K Sharma, Surender Kumar, Poul Nielsen, Jesper Wengel

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: Apr-1
OriginalsprogEngelsk
TidsskriftBioorganic & Medicinal Chemistry Letters
Vol/bind18
Udgave nummer7
Sider (fra-til)2296-2300
Antal sider4
ISSN0960-894X
DOI
StatusUdgivet - 1. apr. 2008

Fingeraftryk

Ribonuclease H
Oligonucleotides
RNA
Complementary RNA
DNA
Nucleosides
Thermodynamic stability

Citer dette

Vester, B., Boel, A. M., Lobedanz, S., Babu, B. R., Raunkjaer, M., Lindegaard, D., ... Wengel, J. (2008). Chemically modified oligonucleotides with efficient RNase H response. Bioorganic & Medicinal Chemistry Letters, 18(7), 2296-2300. https://doi.org/10.1016/j.bmcl.2008.03.004
Vester, Birte ; Boel, Anne Marie ; Lobedanz, Sune ; Babu, B Ravindra ; Raunkjaer, Michael ; Lindegaard, Dorthe ; Raunak ; Hrdlicka, Patrick J ; Højland, Torben ; Sharma, Pawan K ; Kumar, Surender ; Nielsen, Poul ; Wengel, Jesper. / Chemically modified oligonucleotides with efficient RNase H response. I: Bioorganic & Medicinal Chemistry Letters. 2008 ; Bind 18, Nr. 7. s. 2296-2300.
@article{f04a50304e5d11ddb1a1000ea68e967b,
title = "Chemically modified oligonucleotides with efficient RNase H response",
abstract = "Ten different chemically modified nucleosides were incorporated into short DNA strands (chimeric oligonucleotides ON3-ON12 and ON15-ON24) and then tested for their capacity to mediate RNAse H cleavage of the complementary RNA strand. The modifications were placed at two central positions directly in the RNase H cleaving region. The RNA strand of duplexes with ON3, ON5 and ON12 were cleaved more efficiently than the RNA strand of the DNA:RNA control duplex. There seems to be no correlation between the thermal stability between the duplexes and RNase H cleavage.",
keywords = "Base Sequence, Binding Sites, DNA, Nucleic Acid Conformation, Oligonucleotides, RNA, Ribonuclease H, Substrate Specificity",
author = "Birte Vester and Boel, {Anne Marie} and Sune Lobedanz and Babu, {B Ravindra} and Michael Raunkjaer and Dorthe Lindegaard and Raunak and Hrdlicka, {Patrick J} and Torben H{\o}jland and Sharma, {Pawan K} and Surender Kumar and Poul Nielsen and Jesper Wengel",
year = "2008",
month = "4",
day = "1",
doi = "10.1016/j.bmcl.2008.03.004",
language = "English",
volume = "18",
pages = "2296--2300",
journal = "Bioorganic & Medicinal Chemistry Letters",
issn = "0960-894X",
publisher = "Pergamon Press",
number = "7",

}

Vester, B, Boel, AM, Lobedanz, S, Babu, BR, Raunkjaer, M, Lindegaard, D, Raunak, Hrdlicka, PJ, Højland, T, Sharma, PK, Kumar, S, Nielsen, P & Wengel, J 2008, 'Chemically modified oligonucleotides with efficient RNase H response', Bioorganic & Medicinal Chemistry Letters, bind 18, nr. 7, s. 2296-2300. https://doi.org/10.1016/j.bmcl.2008.03.004

Chemically modified oligonucleotides with efficient RNase H response. / Vester, Birte; Boel, Anne Marie; Lobedanz, Sune; Babu, B Ravindra; Raunkjaer, Michael; Lindegaard, Dorthe; Raunak; Hrdlicka, Patrick J; Højland, Torben; Sharma, Pawan K; Kumar, Surender; Nielsen, Poul; Wengel, Jesper.

I: Bioorganic & Medicinal Chemistry Letters, Bind 18, Nr. 7, 01.04.2008, s. 2296-2300.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Chemically modified oligonucleotides with efficient RNase H response

AU - Vester, Birte

AU - Boel, Anne Marie

AU - Lobedanz, Sune

AU - Babu, B Ravindra

AU - Raunkjaer, Michael

AU - Lindegaard, Dorthe

AU - Raunak, null

AU - Hrdlicka, Patrick J

AU - Højland, Torben

AU - Sharma, Pawan K

AU - Kumar, Surender

AU - Nielsen, Poul

AU - Wengel, Jesper

PY - 2008/4/1

Y1 - 2008/4/1

N2 - Ten different chemically modified nucleosides were incorporated into short DNA strands (chimeric oligonucleotides ON3-ON12 and ON15-ON24) and then tested for their capacity to mediate RNAse H cleavage of the complementary RNA strand. The modifications were placed at two central positions directly in the RNase H cleaving region. The RNA strand of duplexes with ON3, ON5 and ON12 were cleaved more efficiently than the RNA strand of the DNA:RNA control duplex. There seems to be no correlation between the thermal stability between the duplexes and RNase H cleavage.

AB - Ten different chemically modified nucleosides were incorporated into short DNA strands (chimeric oligonucleotides ON3-ON12 and ON15-ON24) and then tested for their capacity to mediate RNAse H cleavage of the complementary RNA strand. The modifications were placed at two central positions directly in the RNase H cleaving region. The RNA strand of duplexes with ON3, ON5 and ON12 were cleaved more efficiently than the RNA strand of the DNA:RNA control duplex. There seems to be no correlation between the thermal stability between the duplexes and RNase H cleavage.

KW - Base Sequence

KW - Binding Sites

KW - DNA

KW - Nucleic Acid Conformation

KW - Oligonucleotides

KW - RNA

KW - Ribonuclease H

KW - Substrate Specificity

U2 - 10.1016/j.bmcl.2008.03.004

DO - 10.1016/j.bmcl.2008.03.004

M3 - Journal article

VL - 18

SP - 2296

EP - 2300

JO - Bioorganic & Medicinal Chemistry Letters

JF - Bioorganic & Medicinal Chemistry Letters

SN - 0960-894X

IS - 7

ER -

Vester B, Boel AM, Lobedanz S, Babu BR, Raunkjaer M, Lindegaard D et al. Chemically modified oligonucleotides with efficient RNase H response. Bioorganic & Medicinal Chemistry Letters. 2008 apr 1;18(7):2296-2300. https://doi.org/10.1016/j.bmcl.2008.03.004