Charges in phospholipid layers: Biophysical Journal

Malgorzata Hermanowska, Goran Bijelic, Corina Ciobanasu, Ulrich Kubitscheck, Per Claesson, Beate Maria Klösgen-Buchkremer

Publikation: AndetAndet bidragForskning

Resumé

Interaction between the lipid bilayer and different substrates is often dependant on the bio-physico-chemical properties of the interface. Surface of a membrane is the first site of interaction. The access to the bilayer and its local structural modifications upon interaction with an adsorbing guest molecule are influenced significantly by the presence of charges, and local changes in surface charge density. To investigate these properties we chose model mono- and bilayers made of zwitterionic POPC (and 1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine) and cationic E-POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine). In the first step of our studies we performed isothermal compression of POPC monolayers into which charges were introduced by addition of the ethylated lipid derivative (EPOPC). Charge effects on POPC monolayers at the air-water interface were investigated by addition of different percentages of the cationic EPOPC. The isotherms obtained for the mixtures are compared with pure POPC monolayer isotherms as a reference system. As a second step, for better understanding of this system, diffusion within a POPC bilayer with the same charge addition as for the monolayer study was investigated. For that single molecular tracking analysis on giant unilamellar vesicles was done. Initial results suggest linear decrease in the diffusion coefficient of the dye molecule (DiI-C18). Presented results and future work will be focused on transfer of the phospholipid monolayers to hydrogel support cushion formed on solid support.
OriginalsprogEngelsk
Publikationsdato2009
DOI
StatusUdgivet - 2009

Fingeraftryk

Monolayers
Phospholipids
Isotherms
Unilamellar Liposomes
Molecules
Lipid bilayers
Hydrogel
Surface charge
Charge density
Chemical properties
Coloring Agents
Derivatives
Membranes
Lipids
Water
Substrates
Air
1-palmitoyl-2-oleoylphosphatidylcholine

Citer dette

Hermanowska, M., Bijelic, G., Ciobanasu, C., Kubitscheck, U., Claesson, P., & Klösgen-Buchkremer, B. M. (2009). Charges in phospholipid layers: Biophysical Journal. https://doi.org/10.1016/j.bpj.2008.12.994
Hermanowska, Malgorzata ; Bijelic, Goran ; Ciobanasu, Corina ; Kubitscheck, Ulrich ; Claesson, Per ; Klösgen-Buchkremer, Beate Maria. / Charges in phospholipid layers : Biophysical Journal. 2009.
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abstract = "Interaction between the lipid bilayer and different substrates is often dependant on the bio-physico-chemical properties of the interface. Surface of a membrane is the first site of interaction. The access to the bilayer and its local structural modifications upon interaction with an adsorbing guest molecule are influenced significantly by the presence of charges, and local changes in surface charge density. To investigate these properties we chose model mono- and bilayers made of zwitterionic POPC (and 1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine) and cationic E-POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine). In the first step of our studies we performed isothermal compression of POPC monolayers into which charges were introduced by addition of the ethylated lipid derivative (EPOPC). Charge effects on POPC monolayers at the air-water interface were investigated by addition of different percentages of the cationic EPOPC. The isotherms obtained for the mixtures are compared with pure POPC monolayer isotherms as a reference system. As a second step, for better understanding of this system, diffusion within a POPC bilayer with the same charge addition as for the monolayer study was investigated. For that single molecular tracking analysis on giant unilamellar vesicles was done. Initial results suggest linear decrease in the diffusion coefficient of the dye molecule (DiI-C18). Presented results and future work will be focused on transfer of the phospholipid monolayers to hydrogel support cushion formed on solid support.",
author = "Malgorzata Hermanowska and Goran Bijelic and Corina Ciobanasu and Ulrich Kubitscheck and Per Claesson and Kl{\"o}sgen-Buchkremer, {Beate Maria}",
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Hermanowska, M, Bijelic, G, Ciobanasu, C, Kubitscheck, U, Claesson, P & Klösgen-Buchkremer, BM 2009, Charges in phospholipid layers: Biophysical Journal.. https://doi.org/10.1016/j.bpj.2008.12.994

Charges in phospholipid layers : Biophysical Journal. / Hermanowska, Malgorzata; Bijelic, Goran; Ciobanasu, Corina; Kubitscheck, Ulrich; Claesson, Per; Klösgen-Buchkremer, Beate Maria.

2009, .

Publikation: AndetAndet bidragForskning

TY - GEN

T1 - Charges in phospholipid layers

T2 - Biophysical Journal

AU - Hermanowska, Malgorzata

AU - Bijelic, Goran

AU - Ciobanasu, Corina

AU - Kubitscheck, Ulrich

AU - Claesson, Per

AU - Klösgen-Buchkremer, Beate Maria

PY - 2009

Y1 - 2009

N2 - Interaction between the lipid bilayer and different substrates is often dependant on the bio-physico-chemical properties of the interface. Surface of a membrane is the first site of interaction. The access to the bilayer and its local structural modifications upon interaction with an adsorbing guest molecule are influenced significantly by the presence of charges, and local changes in surface charge density. To investigate these properties we chose model mono- and bilayers made of zwitterionic POPC (and 1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine) and cationic E-POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine). In the first step of our studies we performed isothermal compression of POPC monolayers into which charges were introduced by addition of the ethylated lipid derivative (EPOPC). Charge effects on POPC monolayers at the air-water interface were investigated by addition of different percentages of the cationic EPOPC. The isotherms obtained for the mixtures are compared with pure POPC monolayer isotherms as a reference system. As a second step, for better understanding of this system, diffusion within a POPC bilayer with the same charge addition as for the monolayer study was investigated. For that single molecular tracking analysis on giant unilamellar vesicles was done. Initial results suggest linear decrease in the diffusion coefficient of the dye molecule (DiI-C18). Presented results and future work will be focused on transfer of the phospholipid monolayers to hydrogel support cushion formed on solid support.

AB - Interaction between the lipid bilayer and different substrates is often dependant on the bio-physico-chemical properties of the interface. Surface of a membrane is the first site of interaction. The access to the bilayer and its local structural modifications upon interaction with an adsorbing guest molecule are influenced significantly by the presence of charges, and local changes in surface charge density. To investigate these properties we chose model mono- and bilayers made of zwitterionic POPC (and 1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine) and cationic E-POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine). In the first step of our studies we performed isothermal compression of POPC monolayers into which charges were introduced by addition of the ethylated lipid derivative (EPOPC). Charge effects on POPC monolayers at the air-water interface were investigated by addition of different percentages of the cationic EPOPC. The isotherms obtained for the mixtures are compared with pure POPC monolayer isotherms as a reference system. As a second step, for better understanding of this system, diffusion within a POPC bilayer with the same charge addition as for the monolayer study was investigated. For that single molecular tracking analysis on giant unilamellar vesicles was done. Initial results suggest linear decrease in the diffusion coefficient of the dye molecule (DiI-C18). Presented results and future work will be focused on transfer of the phospholipid monolayers to hydrogel support cushion formed on solid support.

U2 - 10.1016/j.bpj.2008.12.994

DO - 10.1016/j.bpj.2008.12.994

M3 - Other contribution

ER -