Characterizing disease-associated changes in post-translational modifications by mass spectrometry

Camilla Thygesen, Inga Boll, Bente Finsen, Maciej Modzel, Martin R. Larsen*

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Publikation: Bidrag til tidsskriftReviewForskningpeer review

Resumé

Introduction: Exploring post-translational modifications (PTMs) with the use of mass spectrometry (PTMomics) is a rapidly developing area, with methods for discovery/quantification being developed and advanced on a regular basis. PTMs are highly important for the regulation of protein function, interaction and activity, both in physiological and disease states. Changes in PTMs can either cause, or be the result of a disease, making them central for biomarker studies and studies of disease pathogenesis. Recently, it became possible to study multiple PTMs simultaneously from low amount of sample material, thereby increasing coverage of the PTMome obtainable from a single sample. Thus, quantitative PTMomics holds great potential to discover biomarkers from tissue and body fluids as well as elucidating disease mechanisms through characterization of signaling pathways. Areas covered: Recent mass spectrometry-based methods for assessment of the PTMome, with focus on the most studied PTMs, are highlighted. Furthermore, both data dependent and data independent acquisition methods are evaluated. Finally, current challenges in the field are discussed. Expert commentary: PTMomics holds great potential for clinical and biomedical research, especially with the generation of spectral libraries of peptides and PTMs from individual patients (permanent PTM maps) for use in personalized medicine.

OriginalsprogEngelsk
TidsskriftExpert Review of Proteomics
Vol/bind15
Udgave nummer3
Sider (fra-til)245-258
ISSN1478-9450
DOI
StatusUdgivet - 2018

Fingeraftryk

Mass spectrometry
Biomarkers
Peptide Library
Body fluids
Medicine
Precision Medicine
Tissue
Biomedical Research
Proteins

Citer dette

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author = "Camilla Thygesen and Inga Boll and Bente Finsen and Maciej Modzel and Larsen, {Martin R.}",
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Characterizing disease-associated changes in post-translational modifications by mass spectrometry. / Thygesen, Camilla; Boll, Inga; Finsen, Bente; Modzel, Maciej; Larsen, Martin R.

I: Expert Review of Proteomics, Bind 15, Nr. 3, 2018, s. 245-258.

Publikation: Bidrag til tidsskriftReviewForskningpeer review

TY - JOUR

T1 - Characterizing disease-associated changes in post-translational modifications by mass spectrometry

AU - Thygesen, Camilla

AU - Boll, Inga

AU - Finsen, Bente

AU - Modzel, Maciej

AU - Larsen, Martin R.

PY - 2018

Y1 - 2018

N2 - Introduction: Exploring post-translational modifications (PTMs) with the use of mass spectrometry (PTMomics) is a rapidly developing area, with methods for discovery/quantification being developed and advanced on a regular basis. PTMs are highly important for the regulation of protein function, interaction and activity, both in physiological and disease states. Changes in PTMs can either cause, or be the result of a disease, making them central for biomarker studies and studies of disease pathogenesis. Recently, it became possible to study multiple PTMs simultaneously from low amount of sample material, thereby increasing coverage of the PTMome obtainable from a single sample. Thus, quantitative PTMomics holds great potential to discover biomarkers from tissue and body fluids as well as elucidating disease mechanisms through characterization of signaling pathways. Areas covered: Recent mass spectrometry-based methods for assessment of the PTMome, with focus on the most studied PTMs, are highlighted. Furthermore, both data dependent and data independent acquisition methods are evaluated. Finally, current challenges in the field are discussed. Expert commentary: PTMomics holds great potential for clinical and biomedical research, especially with the generation of spectral libraries of peptides and PTMs from individual patients (permanent PTM maps) for use in personalized medicine.

AB - Introduction: Exploring post-translational modifications (PTMs) with the use of mass spectrometry (PTMomics) is a rapidly developing area, with methods for discovery/quantification being developed and advanced on a regular basis. PTMs are highly important for the regulation of protein function, interaction and activity, both in physiological and disease states. Changes in PTMs can either cause, or be the result of a disease, making them central for biomarker studies and studies of disease pathogenesis. Recently, it became possible to study multiple PTMs simultaneously from low amount of sample material, thereby increasing coverage of the PTMome obtainable from a single sample. Thus, quantitative PTMomics holds great potential to discover biomarkers from tissue and body fluids as well as elucidating disease mechanisms through characterization of signaling pathways. Areas covered: Recent mass spectrometry-based methods for assessment of the PTMome, with focus on the most studied PTMs, are highlighted. Furthermore, both data dependent and data independent acquisition methods are evaluated. Finally, current challenges in the field are discussed. Expert commentary: PTMomics holds great potential for clinical and biomedical research, especially with the generation of spectral libraries of peptides and PTMs from individual patients (permanent PTM maps) for use in personalized medicine.

KW - clinical proteomics

KW - DDA strategies

KW - DIA strategies

KW - enrichment strategies

KW - mass spectrometry

KW - Post-translational modification

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DO - 10.1080/14789450.2018.1433036

M3 - Review

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JO - Expert Review of Proteomics

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