Characterization of the phosphoproteome and sialoproteome in brain tissues by mass spectrometry

María Ibáñez-Vea, Stefan J. Kempf, Martin R. Larsen*

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til bog/antologi/rapport/konference-proceedingBidrag til bog/antologiForskningpeer review

Resumé

Mass spectrometry is an essential tool for the characterization of proteins within neuroscience. The development of faster instruments enables neuroscientists to investigate a large proportion of the proteome in the brain in only short analysis time. Yet, a detailed functional investigation of the intrinsic biochemical processes of the brain by evaluation of the post-translational modifications in proteins is still missing. Phosphorylation and N-linked glycosylation are important protein modifications within the brain as they are involved in neural development, neurotransmission, neurite extension, and synaptic plasticity. Although the importance of these protein modifications is undoubtable for the brain functionality, only a few global protein modification datasets have been generated so far in the neuroscience field. This is due to the higher difficulties to sensitively and specifically enrich these low abundant protein modifications from the high abundant nonmodified peptides and from the very lipid-rich brain material. Here, we describe how a highly selective, sensitive, low hands-on-time and cost-effective simultaneous enrichment of phosphorylated peptides, sialylated N-linked glycopeptides as well as intact sialylated N-linked glycopeptides and unmodified peptides from the same biological sample can be applied to bridge this gap in neuroscience, exemplified by a proteomic characterization of the murine brain growth spurt.

OriginalsprogEngelsk
TitelCurrent Proteomic Approaches Applied to Brain Function
RedaktørerEnrique Santamaría, Joaquín Fernández-Irigoyen
ForlagHumana Press
Publikationsdato2017
Sider191-206
ISBN (Trykt)978-1-4939-7118-3
ISBN (Elektronisk)978-1-4939-7119-0
DOI
StatusUdgivet - 2017
NavnNeuromethods
Vol/bind127
ISSN0893-2336

Fingeraftryk

Mass spectrometry
Brain
Tissue
Proteins
Glycopeptides
Peptides
Glycosylation
Phosphorylation
Proteome
Plasticity
Lipids
Costs

Citer dette

Ibáñez-Vea, M., Kempf, S. J., & Larsen, M. R. (2017). Characterization of the phosphoproteome and sialoproteome in brain tissues by mass spectrometry. I E. Santamaría, & J. Fernández-Irigoyen (red.), Current Proteomic Approaches Applied to Brain Function (s. 191-206). Humana Press. Neuromethods, Bind. 127 https://doi.org/10.1007/978-1-4939-7119-0_12
Ibáñez-Vea, María ; Kempf, Stefan J. ; Larsen, Martin R. / Characterization of the phosphoproteome and sialoproteome in brain tissues by mass spectrometry. Current Proteomic Approaches Applied to Brain Function. red. / Enrique Santamaría ; Joaquín Fernández-Irigoyen. Humana Press, 2017. s. 191-206 (Neuromethods, Bind 127).
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abstract = "Mass spectrometry is an essential tool for the characterization of proteins within neuroscience. The development of faster instruments enables neuroscientists to investigate a large proportion of the proteome in the brain in only short analysis time. Yet, a detailed functional investigation of the intrinsic biochemical processes of the brain by evaluation of the post-translational modifications in proteins is still missing. Phosphorylation and N-linked glycosylation are important protein modifications within the brain as they are involved in neural development, neurotransmission, neurite extension, and synaptic plasticity. Although the importance of these protein modifications is undoubtable for the brain functionality, only a few global protein modification datasets have been generated so far in the neuroscience field. This is due to the higher difficulties to sensitively and specifically enrich these low abundant protein modifications from the high abundant nonmodified peptides and from the very lipid-rich brain material. Here, we describe how a highly selective, sensitive, low hands-on-time and cost-effective simultaneous enrichment of phosphorylated peptides, sialylated N-linked glycopeptides as well as intact sialylated N-linked glycopeptides and unmodified peptides from the same biological sample can be applied to bridge this gap in neuroscience, exemplified by a proteomic characterization of the murine brain growth spurt.",
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Ibáñez-Vea, M, Kempf, SJ & Larsen, MR 2017, Characterization of the phosphoproteome and sialoproteome in brain tissues by mass spectrometry. i E Santamaría & J Fernández-Irigoyen (red), Current Proteomic Approaches Applied to Brain Function. Humana Press, Neuromethods, bind 127, s. 191-206. https://doi.org/10.1007/978-1-4939-7119-0_12

Characterization of the phosphoproteome and sialoproteome in brain tissues by mass spectrometry. / Ibáñez-Vea, María; Kempf, Stefan J.; Larsen, Martin R.

Current Proteomic Approaches Applied to Brain Function. red. / Enrique Santamaría; Joaquín Fernández-Irigoyen. Humana Press, 2017. s. 191-206 (Neuromethods, Bind 127).

Publikation: Bidrag til bog/antologi/rapport/konference-proceedingBidrag til bog/antologiForskningpeer review

TY - CHAP

T1 - Characterization of the phosphoproteome and sialoproteome in brain tissues by mass spectrometry

AU - Ibáñez-Vea, María

AU - Kempf, Stefan J.

AU - Larsen, Martin R.

PY - 2017

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N2 - Mass spectrometry is an essential tool for the characterization of proteins within neuroscience. The development of faster instruments enables neuroscientists to investigate a large proportion of the proteome in the brain in only short analysis time. Yet, a detailed functional investigation of the intrinsic biochemical processes of the brain by evaluation of the post-translational modifications in proteins is still missing. Phosphorylation and N-linked glycosylation are important protein modifications within the brain as they are involved in neural development, neurotransmission, neurite extension, and synaptic plasticity. Although the importance of these protein modifications is undoubtable for the brain functionality, only a few global protein modification datasets have been generated so far in the neuroscience field. This is due to the higher difficulties to sensitively and specifically enrich these low abundant protein modifications from the high abundant nonmodified peptides and from the very lipid-rich brain material. Here, we describe how a highly selective, sensitive, low hands-on-time and cost-effective simultaneous enrichment of phosphorylated peptides, sialylated N-linked glycopeptides as well as intact sialylated N-linked glycopeptides and unmodified peptides from the same biological sample can be applied to bridge this gap in neuroscience, exemplified by a proteomic characterization of the murine brain growth spurt.

AB - Mass spectrometry is an essential tool for the characterization of proteins within neuroscience. The development of faster instruments enables neuroscientists to investigate a large proportion of the proteome in the brain in only short analysis time. Yet, a detailed functional investigation of the intrinsic biochemical processes of the brain by evaluation of the post-translational modifications in proteins is still missing. Phosphorylation and N-linked glycosylation are important protein modifications within the brain as they are involved in neural development, neurotransmission, neurite extension, and synaptic plasticity. Although the importance of these protein modifications is undoubtable for the brain functionality, only a few global protein modification datasets have been generated so far in the neuroscience field. This is due to the higher difficulties to sensitively and specifically enrich these low abundant protein modifications from the high abundant nonmodified peptides and from the very lipid-rich brain material. Here, we describe how a highly selective, sensitive, low hands-on-time and cost-effective simultaneous enrichment of phosphorylated peptides, sialylated N-linked glycopeptides as well as intact sialylated N-linked glycopeptides and unmodified peptides from the same biological sample can be applied to bridge this gap in neuroscience, exemplified by a proteomic characterization of the murine brain growth spurt.

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BT - Current Proteomic Approaches Applied to Brain Function

A2 - Santamaría, Enrique

A2 - Fernández-Irigoyen, Joaquín

PB - Humana Press

ER -

Ibáñez-Vea M, Kempf SJ, Larsen MR. Characterization of the phosphoproteome and sialoproteome in brain tissues by mass spectrometry. I Santamaría E, Fernández-Irigoyen J, red., Current Proteomic Approaches Applied to Brain Function. Humana Press. 2017. s. 191-206. (Neuromethods, Bind 127). https://doi.org/10.1007/978-1-4939-7119-0_12