Characterization of Differentially Abundant Proteins Among Leishmania (Viannia) braziliensis Strains Isolated From Atypical or Typical Lesions

Bárbara B. Esteves; Marcella N. Melo-Braga; Vladimir Gorshkov; Thiago Verano-Braga; Martin R. Larsen; Célia M.F. Gontijo; Patricia F. Quaresma; Helida M. Andrade

doi:10.3389/fcimb.2022.824968

Characterization of Differentially Abundant Proteins Among Leishmania (Viannia) braziliensis Strains Isolated From Atypical or Typical Lesions

Bárbara B. Esteves, Marcella N. Melo-Braga, Vladimir Gorshkov, Thiago Verano-Braga, Martin R. Larsen, Célia M.F. Gontijo, Patricia F. Quaresma, Helida M. Andrade^*

^*Kontaktforfatter

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Abstract

Leishmania (Viannia) braziliensis is the main etiological agent of cutaneous and mucocutaneous leishmaniasis in Latin America. Non-ulcerated atypical tegumentary leishmaniasis cases caused by L. braziliensis have been reported in several regions of the American continent, including the Xacriabá indigenous reserve in São João das Missões/Minas Gerais, Brazil. Parasites isolated from these atypical clinical lesions are resistant to antimony-based therapeutics. In the present study, proteins displaying differential abundance in two strains of L. braziliensis isolated from patients with atypical lesions compared with four strains isolated from patients with typical lesions were identified using a quantitative proteomics approach based on tandem mass tag labeling (TMT) and mass spectrometry. A total of 532 (P<0.05) differentially abundant proteins were identified (298 upregulated and 234 downregulated) in strains from atypical lesions compared to strains from typical lesions. Prominent positively regulated proteins in atypical strains included those that may confer greater survival inside macrophages, proteins related to antimony resistance, and proteins associated with higher peroxidase activity. Additionally, we identified proteins showing potential as new drug and vaccine targets. Our findings contribute to the characterization of these intriguing L. braziliensis strains and provide a novel perspective on Atypical Cutaneous Leishmaniasis (ACL) cases that have been associated with therapeutic failures.

Originalsprog	Engelsk
Artikelnummer	824968
Tidsskrift	Frontiers in Cellular and Infection Microbiology
Vol/bind	12
ISSN	2235-2988
DOI	https://doi.org/10.3389/fcimb.2022.824968
Status	Udgivet - 15. feb. 2022

Bibliografisk note

Funding Information:
This research was supported by the Fundação de Amparo à Pesquisa do Estado de Minas Gerais [PPM00129-17], Instituto Nacional de Ciência e Tecnologia de Vacinas [CNPq-573547/ 2008-4/FAPEMIG/MS-CBB, APQ 00077-09] and Rede Mineira de Biomoléculas [CCBRED00012-14]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. HA is CNPq fellows (PQ). BE and MM-B are CAPES fellowship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Publisher Copyright:
Copyright © 2022 Esteves, Melo-Braga, Gorshkov, Verano-Braga, Larsen, Gontijo, Quaresma and Andrade.

Dokumenter og links

10.3389/fcimb.2022.824968Licens: CC BY

Open access versionForlagets udgivne version, 32,2 MBLicens: CC BY

Citationsformater

Esteves, B. B., Melo-Braga, M. N., Gorshkov, V., Verano-Braga, T., Larsen, M. R., Gontijo, C. M. F., Quaresma, P. F., & Andrade, H. M. (2022). Characterization of Differentially Abundant Proteins Among Leishmania (Viannia) braziliensis Strains Isolated From Atypical or Typical Lesions. Frontiers in Cellular and Infection Microbiology, 12, Artikel 824968. https://doi.org/10.3389/fcimb.2022.824968

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title = "Characterization of Differentially Abundant Proteins Among Leishmania (Viannia) braziliensis Strains Isolated From Atypical or Typical Lesions",

abstract = "Leishmania (Viannia) braziliensis is the main etiological agent of cutaneous and mucocutaneous leishmaniasis in Latin America. Non-ulcerated atypical tegumentary leishmaniasis cases caused by L. braziliensis have been reported in several regions of the American continent, including the Xacriab{\'a} indigenous reserve in S{\~a}o Jo{\~a}o das Miss{\~o}es/Minas Gerais, Brazil. Parasites isolated from these atypical clinical lesions are resistant to antimony-based therapeutics. In the present study, proteins displaying differential abundance in two strains of L. braziliensis isolated from patients with atypical lesions compared with four strains isolated from patients with typical lesions were identified using a quantitative proteomics approach based on tandem mass tag labeling (TMT) and mass spectrometry. A total of 532 (P<0.05) differentially abundant proteins were identified (298 upregulated and 234 downregulated) in strains from atypical lesions compared to strains from typical lesions. Prominent positively regulated proteins in atypical strains included those that may confer greater survival inside macrophages, proteins related to antimony resistance, and proteins associated with higher peroxidase activity. Additionally, we identified proteins showing potential as new drug and vaccine targets. Our findings contribute to the characterization of these intriguing L. braziliensis strains and provide a novel perspective on Atypical Cutaneous Leishmaniasis (ACL) cases that have been associated with therapeutic failures.",

keywords = "abundance, antimony resistance, atypical wounds, Leishmania braziliensis, proteome, TMT",

author = "Esteves, {B{\'a}rbara B.} and Melo-Braga, {Marcella N.} and Vladimir Gorshkov and Thiago Verano-Braga and Larsen, {Martin R.} and Gontijo, {C{\'e}lia M.F.} and Quaresma, {Patricia F.} and Andrade, {Helida M.}",

note = "Funding Information: This research was supported by the Funda{\c c}{\~a}o de Amparo {\`a} Pesquisa do Estado de Minas Gerais [PPM00129-17], Instituto Nacional de Ci{\^e}ncia e Tecnologia de Vacinas [CNPq-573547/ 2008-4/FAPEMIG/MS-CBB, APQ 00077-09] and Rede Mineira de Biomol{\'e}culas [CCBRED00012-14]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. HA is CNPq fellows (PQ). BE and MM-B are CAPES fellowship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: Copyright {\textcopyright} 2022 Esteves, Melo-Braga, Gorshkov, Verano-Braga, Larsen, Gontijo, Quaresma and Andrade.",

year = "2022",

month = feb,

day = "15",

doi = "10.3389/fcimb.2022.824968",

language = "English",

volume = "12",

journal = "Frontiers in Cellular and Infection Microbiology",

issn = "2235-2988",

publisher = "Frontiers Media",

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Esteves, BB, Melo-Braga, MN, Gorshkov, V, Verano-Braga, T, Larsen, MR, Gontijo, CMF, Quaresma, PF & Andrade, HM 2022, 'Characterization of Differentially Abundant Proteins Among Leishmania (Viannia) braziliensis Strains Isolated From Atypical or Typical Lesions', Frontiers in Cellular and Infection Microbiology, bind 12, 824968. https://doi.org/10.3389/fcimb.2022.824968

Characterization of Differentially Abundant Proteins Among Leishmania (Viannia) braziliensis Strains Isolated From Atypical or Typical Lesions. / Esteves, Bárbara B.; Melo-Braga, Marcella N.; Gorshkov, Vladimir et al.
I: Frontiers in Cellular and Infection Microbiology, Bind 12, 824968, 15.02.2022.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - Characterization of Differentially Abundant Proteins Among Leishmania (Viannia) braziliensis Strains Isolated From Atypical or Typical Lesions

AU - Esteves, Bárbara B.

AU - Melo-Braga, Marcella N.

AU - Gorshkov, Vladimir

AU - Verano-Braga, Thiago

AU - Larsen, Martin R.

AU - Gontijo, Célia M.F.

AU - Quaresma, Patricia F.

AU - Andrade, Helida M.

N1 - Funding Information: This research was supported by the Fundação de Amparo à Pesquisa do Estado de Minas Gerais [PPM00129-17], Instituto Nacional de Ciência e Tecnologia de Vacinas [CNPq-573547/ 2008-4/FAPEMIG/MS-CBB, APQ 00077-09] and Rede Mineira de Biomoléculas [CCBRED00012-14]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. HA is CNPq fellows (PQ). BE and MM-B are CAPES fellowship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: Copyright © 2022 Esteves, Melo-Braga, Gorshkov, Verano-Braga, Larsen, Gontijo, Quaresma and Andrade.

PY - 2022/2/15

Y1 - 2022/2/15

N2 - Leishmania (Viannia) braziliensis is the main etiological agent of cutaneous and mucocutaneous leishmaniasis in Latin America. Non-ulcerated atypical tegumentary leishmaniasis cases caused by L. braziliensis have been reported in several regions of the American continent, including the Xacriabá indigenous reserve in São João das Missões/Minas Gerais, Brazil. Parasites isolated from these atypical clinical lesions are resistant to antimony-based therapeutics. In the present study, proteins displaying differential abundance in two strains of L. braziliensis isolated from patients with atypical lesions compared with four strains isolated from patients with typical lesions were identified using a quantitative proteomics approach based on tandem mass tag labeling (TMT) and mass spectrometry. A total of 532 (P<0.05) differentially abundant proteins were identified (298 upregulated and 234 downregulated) in strains from atypical lesions compared to strains from typical lesions. Prominent positively regulated proteins in atypical strains included those that may confer greater survival inside macrophages, proteins related to antimony resistance, and proteins associated with higher peroxidase activity. Additionally, we identified proteins showing potential as new drug and vaccine targets. Our findings contribute to the characterization of these intriguing L. braziliensis strains and provide a novel perspective on Atypical Cutaneous Leishmaniasis (ACL) cases that have been associated with therapeutic failures.

AB - Leishmania (Viannia) braziliensis is the main etiological agent of cutaneous and mucocutaneous leishmaniasis in Latin America. Non-ulcerated atypical tegumentary leishmaniasis cases caused by L. braziliensis have been reported in several regions of the American continent, including the Xacriabá indigenous reserve in São João das Missões/Minas Gerais, Brazil. Parasites isolated from these atypical clinical lesions are resistant to antimony-based therapeutics. In the present study, proteins displaying differential abundance in two strains of L. braziliensis isolated from patients with atypical lesions compared with four strains isolated from patients with typical lesions were identified using a quantitative proteomics approach based on tandem mass tag labeling (TMT) and mass spectrometry. A total of 532 (P<0.05) differentially abundant proteins were identified (298 upregulated and 234 downregulated) in strains from atypical lesions compared to strains from typical lesions. Prominent positively regulated proteins in atypical strains included those that may confer greater survival inside macrophages, proteins related to antimony resistance, and proteins associated with higher peroxidase activity. Additionally, we identified proteins showing potential as new drug and vaccine targets. Our findings contribute to the characterization of these intriguing L. braziliensis strains and provide a novel perspective on Atypical Cutaneous Leishmaniasis (ACL) cases that have been associated with therapeutic failures.

KW - abundance

KW - antimony resistance

KW - atypical wounds

KW - Leishmania braziliensis

KW - proteome

KW - TMT

U2 - 10.3389/fcimb.2022.824968

DO - 10.3389/fcimb.2022.824968

M3 - Journal article

C2 - 35242720

AN - SCOPUS:85125591556

SN - 2235-2988

VL - 12

JO - Frontiers in Cellular and Infection Microbiology

JF - Frontiers in Cellular and Infection Microbiology

M1 - 824968

ER -

Characterization of Differentially Abundant Proteins Among Leishmania (Viannia) braziliensis Strains Isolated From Atypical or Typical Lesions

Abstract

Bibliografisk note

Dokumenter og links

Fingeraftryk

Citationsformater