Characterization of a New Biomimetic Multilayer System for Biomembrane Interaction Studies: Biophysical Journal

Malgorzata Hermanowska, Jonas Borch, Adam Cohen Simonsen, Beate Klösgen, Beate Maria Klösgen-Buchkremer

Publikation: AndetAndet bidragForskning

Resumé

During the past decade, a lot of focus was placed on investigation of the layer-by-layer (LbL) deposition of polyelectrolyte multilayers (PEM). It is proven to be a good technique for obtaining stable and functional supports for various bio-systems. In our work, we focus on investigation of interaction between chitosan/heparin PEM films and small unilamellar vesicles (SUV). The lipids chosen for this study are mixtures of zwitterionic POPC and cationic E-POPC resulting in a positive surface charge density of the vesicles. The self-assembly process of physisorption of subsequent polyelectrolyte layers was followed by the Surface Plasmon Resonance (SPR) technique, allowing investigation of the deposition efficiency and dynamics. Later the formation of the lipid bilayer on top of the PEM film with both chitosan and heparin as the top layer was investigated. Also the study of the polyelectrolyte film structure was done by atomic force microscopy (AFM). Both dry and fully hydrated films were observed. As the last step of the study, the structure of the lipid bilayer deposited on top of positive and negative PEM film was visualized with the AFM technique. The information obtained from the SPR and AFM experiments will be used for further investigation of the hydration state of the PEM films and the lipid bilayer by neutron reflectivity.
OriginalsprogEngelsk
Publikationsdato2009
DOI
StatusUdgivet - 2009

Fingeraftryk

biomimetics
lipids
heparins
interactions
atomic force microscopy
surface plasmon resonance
hydration
self assembly
reflectance
neutrons

Citer dette

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title = "Characterization of a New Biomimetic Multilayer System for Biomembrane Interaction Studies: Biophysical Journal",
abstract = "During the past decade, a lot of focus was placed on investigation of the layer-by-layer (LbL) deposition of polyelectrolyte multilayers (PEM). It is proven to be a good technique for obtaining stable and functional supports for various bio-systems. In our work, we focus on investigation of interaction between chitosan/heparin PEM films and small unilamellar vesicles (SUV). The lipids chosen for this study are mixtures of zwitterionic POPC and cationic E-POPC resulting in a positive surface charge density of the vesicles. The self-assembly process of physisorption of subsequent polyelectrolyte layers was followed by the Surface Plasmon Resonance (SPR) technique, allowing investigation of the deposition efficiency and dynamics. Later the formation of the lipid bilayer on top of the PEM film with both chitosan and heparin as the top layer was investigated. Also the study of the polyelectrolyte film structure was done by atomic force microscopy (AFM). Both dry and fully hydrated films were observed. As the last step of the study, the structure of the lipid bilayer deposited on top of positive and negative PEM film was visualized with the AFM technique. The information obtained from the SPR and AFM experiments will be used for further investigation of the hydration state of the PEM films and the lipid bilayer by neutron reflectivity.",
author = "Malgorzata Hermanowska and Jonas Borch and Simonsen, {Adam Cohen} and Beate Kl{\"o}sgen and Kl{\"o}sgen-Buchkremer, {Beate Maria}",
year = "2009",
doi = "10.1016/j.bpj.2008.12.2322",
language = "English",
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Characterization of a New Biomimetic Multilayer System for Biomembrane Interaction Studies : Biophysical Journal. / Hermanowska, Malgorzata; Borch, Jonas; Simonsen, Adam Cohen; Klösgen, Beate; Klösgen-Buchkremer, Beate Maria.

2009, .

Publikation: AndetAndet bidragForskning

TY - GEN

T1 - Characterization of a New Biomimetic Multilayer System for Biomembrane Interaction Studies

T2 - Biophysical Journal

AU - Hermanowska, Malgorzata

AU - Borch, Jonas

AU - Simonsen, Adam Cohen

AU - Klösgen, Beate

AU - Klösgen-Buchkremer, Beate Maria

PY - 2009

Y1 - 2009

N2 - During the past decade, a lot of focus was placed on investigation of the layer-by-layer (LbL) deposition of polyelectrolyte multilayers (PEM). It is proven to be a good technique for obtaining stable and functional supports for various bio-systems. In our work, we focus on investigation of interaction between chitosan/heparin PEM films and small unilamellar vesicles (SUV). The lipids chosen for this study are mixtures of zwitterionic POPC and cationic E-POPC resulting in a positive surface charge density of the vesicles. The self-assembly process of physisorption of subsequent polyelectrolyte layers was followed by the Surface Plasmon Resonance (SPR) technique, allowing investigation of the deposition efficiency and dynamics. Later the formation of the lipid bilayer on top of the PEM film with both chitosan and heparin as the top layer was investigated. Also the study of the polyelectrolyte film structure was done by atomic force microscopy (AFM). Both dry and fully hydrated films were observed. As the last step of the study, the structure of the lipid bilayer deposited on top of positive and negative PEM film was visualized with the AFM technique. The information obtained from the SPR and AFM experiments will be used for further investigation of the hydration state of the PEM films and the lipid bilayer by neutron reflectivity.

AB - During the past decade, a lot of focus was placed on investigation of the layer-by-layer (LbL) deposition of polyelectrolyte multilayers (PEM). It is proven to be a good technique for obtaining stable and functional supports for various bio-systems. In our work, we focus on investigation of interaction between chitosan/heparin PEM films and small unilamellar vesicles (SUV). The lipids chosen for this study are mixtures of zwitterionic POPC and cationic E-POPC resulting in a positive surface charge density of the vesicles. The self-assembly process of physisorption of subsequent polyelectrolyte layers was followed by the Surface Plasmon Resonance (SPR) technique, allowing investigation of the deposition efficiency and dynamics. Later the formation of the lipid bilayer on top of the PEM film with both chitosan and heparin as the top layer was investigated. Also the study of the polyelectrolyte film structure was done by atomic force microscopy (AFM). Both dry and fully hydrated films were observed. As the last step of the study, the structure of the lipid bilayer deposited on top of positive and negative PEM film was visualized with the AFM technique. The information obtained from the SPR and AFM experiments will be used for further investigation of the hydration state of the PEM films and the lipid bilayer by neutron reflectivity.

U2 - 10.1016/j.bpj.2008.12.2322

DO - 10.1016/j.bpj.2008.12.2322

M3 - Other contribution

ER -