Cavernous nerve stimulation and recording of intracavernous pressure in a rat

Morten Hox, Travis Mann-Gow, Lars Lund, Peter Zvara*

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

The stimulation of the cavernous nerve (CN) and measurement of intracavernous pressure (ICP) have been used extensively to test and evaluate therapies for erectile dysfunction. However, the methods used vary between laboratories, and pitfalls still exist. The goal of this study was to describe a surgical technique that would provide a reliable and reproducible model. By exposing the ischiocavernosus muscle at its point of insertion on the ischial tuberosity, the penile crus could be cannulated with minimal dissection and injury to the structures involved in erectile function. Repeated stimulation of the CN, without the need for lifting and drying, was achieved by using a 125 µm bipolar silver electrode and biocompatible silicon glue to isolate the electrode-nerve complex. This method prevents neuropraxia by reducing stretching and drying the nerve and provides complete isolation of the nerve, negating electrical leakage and preventing stimulation of alternative pathways.

OriginalsprogEngelsk
Artikelnummere56807
TidsskriftJournal of Visualized Experiments
Vol/bind2018
Udgave nummer134
Antal sider13
ISSN1940-087X
DOI
StatusUdgivet - 23. apr. 2018

Fingeraftryk

Rats
Nerve Expansion
Drying
Dissection
Electrodes
Glues
Silicon
Silver
Adhesives
Stretching
Muscle
Muscles
Wounds and Injuries

Citer dette

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title = "Cavernous nerve stimulation and recording of intracavernous pressure in a rat",
abstract = "The stimulation of the cavernous nerve (CN) and measurement of intracavernous pressure (ICP) have been used extensively to test and evaluate therapies for erectile dysfunction. However, the methods used vary between laboratories, and pitfalls still exist. The goal of this study was to describe a surgical technique that would provide a reliable and reproducible model. By exposing the ischiocavernosus muscle at its point of insertion on the ischial tuberosity, the penile crus could be cannulated with minimal dissection and injury to the structures involved in erectile function. Repeated stimulation of the CN, without the need for lifting and drying, was achieved by using a 125 µm bipolar silver electrode and biocompatible silicon glue to isolate the electrode-nerve complex. This method prevents neuropraxia by reducing stretching and drying the nerve and provides complete isolation of the nerve, negating electrical leakage and preventing stimulation of alternative pathways.",
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author = "Morten Hox and Travis Mann-Gow and Lars Lund and Peter Zvara",
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day = "23",
doi = "10.3791/56807",
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Cavernous nerve stimulation and recording of intracavernous pressure in a rat. / Hox, Morten; Mann-Gow, Travis; Lund, Lars; Zvara, Peter.

I: Journal of Visualized Experiments, Bind 2018, Nr. 134, e56807, 23.04.2018.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

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AU - Mann-Gow, Travis

AU - Lund, Lars

AU - Zvara, Peter

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N2 - The stimulation of the cavernous nerve (CN) and measurement of intracavernous pressure (ICP) have been used extensively to test and evaluate therapies for erectile dysfunction. However, the methods used vary between laboratories, and pitfalls still exist. The goal of this study was to describe a surgical technique that would provide a reliable and reproducible model. By exposing the ischiocavernosus muscle at its point of insertion on the ischial tuberosity, the penile crus could be cannulated with minimal dissection and injury to the structures involved in erectile function. Repeated stimulation of the CN, without the need for lifting and drying, was achieved by using a 125 µm bipolar silver electrode and biocompatible silicon glue to isolate the electrode-nerve complex. This method prevents neuropraxia by reducing stretching and drying the nerve and provides complete isolation of the nerve, negating electrical leakage and preventing stimulation of alternative pathways.

AB - The stimulation of the cavernous nerve (CN) and measurement of intracavernous pressure (ICP) have been used extensively to test and evaluate therapies for erectile dysfunction. However, the methods used vary between laboratories, and pitfalls still exist. The goal of this study was to describe a surgical technique that would provide a reliable and reproducible model. By exposing the ischiocavernosus muscle at its point of insertion on the ischial tuberosity, the penile crus could be cannulated with minimal dissection and injury to the structures involved in erectile function. Repeated stimulation of the CN, without the need for lifting and drying, was achieved by using a 125 µm bipolar silver electrode and biocompatible silicon glue to isolate the electrode-nerve complex. This method prevents neuropraxia by reducing stretching and drying the nerve and provides complete isolation of the nerve, negating electrical leakage and preventing stimulation of alternative pathways.

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KW - Crus dissection

KW - Erectile dysfunction

KW - Intracavernous pressure measurement

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KW - Medicine

KW - Oxygenation

KW - Rat

KW - Electric Stimulation/methods

KW - Rats

KW - Male

KW - Erectile Dysfunction/diagnosis

KW - Rats, Sprague-Dawley

KW - Animals

KW - Cavernous Sinus/innervation

KW - Disease Models, Animal

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