Castor Oil Transesterification Catalysed by Liquid Enzymes

Feasibility of Reuse under Various Reaction Conditions

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Resumé

In the present work, biodiesel production by reaction of non-edible castor oil with methanol under enzymatic catalysis is investigated. Two liquid enzymes were tested: Eversa Transform and Resinase HT. Reactions were performed at 35 °C and with a molar ratio of methanol to oil of 6:1. The reaction time was 8 hours.
Stepwise addition of methanol was necessary to avoid enzyme inhibition by methanol. In order to minimize the enzyme costs, the influence of enzyme activity loss during reuse of both enzymes was evaluated under two distinct conditions. In the former, the enzymes were recovered and fully reused; in the latter, a mixture of 50 % reused and 50 % fresh enzymes was tested. In the case of total reuse after three cycles, both enzymes achieved only low conversions. The biodiesel content in the oil-phase using Eversa Transform was 94.21 %
for the first cycle, 68.39 % in the second, and 33.35 % in the third cycle. Resinase HT gave 88.05, 86.60 and 58.24 %, respectively. For the partial enzyme reuse, after three cycles, the biodiesel content was 88.36 and 88.05 % respectively for the Eversa Transform and Resinase HT, showing that partial reuse of enzymes could be beneficial for enzymatic transesterification reactions.
OriginalsprogEngelsk
BogserieChemical engineering transactions
Vol/bind57
Sider (fra-til)913-918
ISSN1974-9791
DOI
StatusUdgivet - 2017
Begivenhed13th International Conference on Chemical and Process Engineering - Hotel Michelangelo,Piazza Luigi di Savoia, 6 , Milano, Italien
Varighed: 28. maj 201731. maj 2017
Konferencens nummer: 13

Konference

Konference13th International Conference on Chemical and Process Engineering
Nummer13
LokationHotel Michelangelo,Piazza Luigi di Savoia, 6
LandItalien
ByMilano
Periode28/05/201731/05/2017

Fingeraftryk

Castor Oil
Transesterification
Enzymes
Liquids
Methanol
Biofuels
Biodiesel
Mathematical transformations
Oils
Enzyme inhibition
Enzyme activity
Catalysis

Citer dette

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title = "Castor Oil Transesterification Catalysed by Liquid Enzymes: Feasibility of Reuse under Various Reaction Conditions",
abstract = "In the present work, biodiesel production by reaction of non-edible castor oil with methanol under enzymatic catalysis is investigated. Two liquid enzymes were tested: Eversa Transform and Resinase HT. Reactions were performed at 35 °C and with a molar ratio of methanol to oil of 6:1. The reaction time was 8 hours.Stepwise addition of methanol was necessary to avoid enzyme inhibition by methanol. In order to minimize the enzyme costs, the influence of enzyme activity loss during reuse of both enzymes was evaluated under two distinct conditions. In the former, the enzymes were recovered and fully reused; in the latter, a mixture of 50 {\%} reused and 50 {\%} fresh enzymes was tested. In the case of total reuse after three cycles, both enzymes achieved only low conversions. The biodiesel content in the oil-phase using Eversa Transform was 94.21 {\%}for the first cycle, 68.39 {\%} in the second, and 33.35 {\%} in the third cycle. Resinase HT gave 88.05, 86.60 and 58.24 {\%}, respectively. For the partial enzyme reuse, after three cycles, the biodiesel content was 88.36 and 88.05 {\%} respectively for the Eversa Transform and Resinase HT, showing that partial reuse of enzymes could be beneficial for enzymatic transesterification reactions.",
author = "Thalles Andrade and Massimiliano Errico and Christensen, {Knud Villy}",
year = "2017",
doi = "10.3303/CET1757153",
language = "English",
volume = "57",
pages = "913--918",
journal = "Chemical engineering transactions",
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TY - GEN

T1 - Castor Oil Transesterification Catalysed by Liquid Enzymes

T2 - Feasibility of Reuse under Various Reaction Conditions

AU - Andrade, Thalles

AU - Errico, Massimiliano

AU - Christensen, Knud Villy

PY - 2017

Y1 - 2017

N2 - In the present work, biodiesel production by reaction of non-edible castor oil with methanol under enzymatic catalysis is investigated. Two liquid enzymes were tested: Eversa Transform and Resinase HT. Reactions were performed at 35 °C and with a molar ratio of methanol to oil of 6:1. The reaction time was 8 hours.Stepwise addition of methanol was necessary to avoid enzyme inhibition by methanol. In order to minimize the enzyme costs, the influence of enzyme activity loss during reuse of both enzymes was evaluated under two distinct conditions. In the former, the enzymes were recovered and fully reused; in the latter, a mixture of 50 % reused and 50 % fresh enzymes was tested. In the case of total reuse after three cycles, both enzymes achieved only low conversions. The biodiesel content in the oil-phase using Eversa Transform was 94.21 %for the first cycle, 68.39 % in the second, and 33.35 % in the third cycle. Resinase HT gave 88.05, 86.60 and 58.24 %, respectively. For the partial enzyme reuse, after three cycles, the biodiesel content was 88.36 and 88.05 % respectively for the Eversa Transform and Resinase HT, showing that partial reuse of enzymes could be beneficial for enzymatic transesterification reactions.

AB - In the present work, biodiesel production by reaction of non-edible castor oil with methanol under enzymatic catalysis is investigated. Two liquid enzymes were tested: Eversa Transform and Resinase HT. Reactions were performed at 35 °C and with a molar ratio of methanol to oil of 6:1. The reaction time was 8 hours.Stepwise addition of methanol was necessary to avoid enzyme inhibition by methanol. In order to minimize the enzyme costs, the influence of enzyme activity loss during reuse of both enzymes was evaluated under two distinct conditions. In the former, the enzymes were recovered and fully reused; in the latter, a mixture of 50 % reused and 50 % fresh enzymes was tested. In the case of total reuse after three cycles, both enzymes achieved only low conversions. The biodiesel content in the oil-phase using Eversa Transform was 94.21 %for the first cycle, 68.39 % in the second, and 33.35 % in the third cycle. Resinase HT gave 88.05, 86.60 and 58.24 %, respectively. For the partial enzyme reuse, after three cycles, the biodiesel content was 88.36 and 88.05 % respectively for the Eversa Transform and Resinase HT, showing that partial reuse of enzymes could be beneficial for enzymatic transesterification reactions.

U2 - 10.3303/CET1757153

DO - 10.3303/CET1757153

M3 - Conference article

VL - 57

SP - 913

EP - 918

JO - Chemical engineering transactions

JF - Chemical engineering transactions

SN - 1974-9791

ER -