Abstract
The objective of this study was to investigate the suitability of carbohydrate plasma volume expanders as
a novel polymer platform for tumor targeting. Many synthetic polymers have already been synthesized for
targeted tumor therapy, but potential advantages of these carbohydrates include inexpensive synthesis,
constant availability, a good safety profile, biodegradability and the long clinical use as plasma expanders.
Three polymers have been tested for cytotoxicity and cytokine activation in cell cultures and conjugated
with a near-infrared fluorescent dye: hydroxyethyl starches (HES 200 kDa and HES 450 kDa) and dextran
(DEX 500 kDa). Particle size and molecular weight distribution were determined by asymmetric flow
field-flow fractionation (AF4). The biodistribution was investigated non-invasively in nude mice using
multispectral optical imaging. The most promising polymer conjugate was characterized in human colon
carcinoma xenograft bearing nude mice. A tumor specific accumulation of HES 450 was observed, which
proves it’s potential as carrier for passive tumor targeting.
a novel polymer platform for tumor targeting. Many synthetic polymers have already been synthesized for
targeted tumor therapy, but potential advantages of these carbohydrates include inexpensive synthesis,
constant availability, a good safety profile, biodegradability and the long clinical use as plasma expanders.
Three polymers have been tested for cytotoxicity and cytokine activation in cell cultures and conjugated
with a near-infrared fluorescent dye: hydroxyethyl starches (HES 200 kDa and HES 450 kDa) and dextran
(DEX 500 kDa). Particle size and molecular weight distribution were determined by asymmetric flow
field-flow fractionation (AF4). The biodistribution was investigated non-invasively in nude mice using
multispectral optical imaging. The most promising polymer conjugate was characterized in human colon
carcinoma xenograft bearing nude mice. A tumor specific accumulation of HES 450 was observed, which
proves it’s potential as carrier for passive tumor targeting.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Carbohydrate Polymers |
| Vol/bind | 95 |
| Sider (fra-til) | 404-413 |
| Antal sider | 10 |
| ISSN | 0144-8617 |
| DOI | |
| Status | Udgivet - 2013 |