Can Vitamin B12 Assist the Internalization of Antisense LNA Oligonucleotides into Bacteria?

Sara Pereira, Ruwei Yao, Mariana Gomes, Per Trolle Jørgensen, Jesper Wengel, Nuno Filipe Azevedo, Rita Sobral Santos*

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Abstrakt

Abstract: The emergence of bacterial resistance to traditional small-molecule antibiotics is fueling the search for innovative strategies to treat infections. Inhibiting the expression of essential bacterial genes using antisense oligonucleotides (ASOs), particularly composed of nucleic acid mimics (NAMs), has emerged as a promising strategy. However, their efficiency depends on their association with vectors that can translocate the bacterial envelope. Vitamin B12 is among the largest molecules known to be taken up by bacteria and has very recently started to gain interest as a trojan-horse vector. Gapmers and steric blockers were evaluated as ASOs against Escherichia coli (E. coli). Both ASOs were successfully conjugated to B12 by copper-free azide-alkyne click-chemistry. The biological effect of the two conjugates was evaluated together with their intracellular localization in E. coli. Although not only B12 but also both B12-ASO conjugates interacted strongly with E. coli, they were mostly colocalized with the outer membrane. Only 6-9% were detected in the cytosol, which showed to be insufficient for bacterial growth inhibition. These results suggest that the internalization of B12-ASO conjugates is strongly affected by the low uptake rate of the B12 in E. coli and that further studies are needed before considering this strategy against biofilms in vivo.

OriginalsprogEngelsk
Artikelnummer379
TidsskriftAntibiotics
Vol/bind10
Udgave nummer4
Antal sider12
DOI
StatusUdgivet - apr. 2021

Bibliografisk note

Funding Information:
The research was funded by Funda??o para a Ci?ncia e Tecnologia, PhD grant SFRH/BD/ 118018/2016; the Project UID/EQU/00511/2019-Laboratory for Process Engineering, Environment, Biotechnology and Energy-LEPABE funded by national funds through FCT/MCTES (PIDDAC); Project ?LEPABE-2-ECO-INNOVATION?-NORTE-01-0145-FEDER-000005, funded by Norte Portugal Regional Operational Programme (NORTE 2020), under PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF); and the European Union?s Horizon 2020 research and innovation program under grant agreement No 810685; Biomolecular Nanoscale Engineering Center (BioNEC), a VILLUM center of excellence, funded by VILLUM FONDEN, grant number VKR18333. Joan Hansen and Tina Grubbe from BioNEC are thanked for technical assistance.

Publisher Copyright:
© 2021 by the authors.

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