Calibration, specificity and trueness of a postheparin plasma lipoprotein lipase assay

F. L. Henriksen*, P. H. Petersen, H. Beck-Nielsen, M. Hørder

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Abstrakt

Measurement of lipoprotein lipase activity in postheparin plasma is generally accompanied by moderate within-run variation CVW-R (<10%) and higher between-run variation CVB-R (5-25%). A calibration system was introduced in order to improve the reproducibility of measurements and to compare lipoprotein lipase activities from different days. Every day a calibration curve for lipoprotein lipase activity was constructed. Fifteen calibration curves designed over 2 years, show linearity over the whole biological spectrum and a considerable reduction of between-run variation in lipoprotein lipase activity, from 42% to 5.3% as estimated from two control postheparin plasma samples. The lipoprotein lipase calibration system is an easy and very cheap arrangement, which makes it possible to compare lipoprotein lipase activities achieved over years. When the lipoprotein lipase control values are compared with reference lipoprotein lipase samples determined in other lipase laboratories, the calibration-control system becomes an important tool for reducing analytical bias. The article reviews the original analytical criteria of catalytic measurement of lipoprotein lipase activity and describes the implementation of the calibration-control system. We describe a model for reduction of the analytical variability in the measurement of lipoprotein lipase activity. Other standardization efforts need to be made in the future, especially to define the reference material for calibration.

OriginalsprogEngelsk
TidsskriftClinical Chemistry and Laboratory Medicine
Vol/bind39
Udgave nummer3
Sider (fra-til)263-269
Antal sider7
ISSN1434-6621
DOI
StatusUdgivet - 12. maj 2001

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