Calibration of matrix-assisted laser desorption/ionization time-of-flight peptide mass fingerprinting spectra

Karin Hjernø, Peter Højrup

Publikation: Kapitel i bog/rapport/konference-proceedingKapitel i bogForskning

Abstract

This chapter describes a number of aspects important for calibration of matrix-assisted laser desorption/ionization time-of-flight spectra prior to peptide mass fingerprinting searches. Both multipoint internal calibration and mass defect-based calibration is illustrated. The chapter describes how potential internal calibrants, like tryptic autodigest peptides and keratin-related peptides, can be identified and used for high-precision calibration. Furthermore, the construction of project/user-specific lists of potential calibrants is illustrated.
OriginalsprogEngelsk
TitelMethods in Molecular Biology : Mass Spectrometry Data Analysis in Proteomics
RedaktørerRune Matthiesen
Antal sider11
Vol/bind367
ForlagAAAI Press
Publikationsdato2007
Udgave1
Sider49-60
ISBN (Trykt)978-1-58829-563-7
ISBN (Elektronisk)978-1-59745-275-5
DOI
StatusUdgivet - 2007

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