TY - JOUR
T1 - Branched oligonucleotides containing bicyclic nucleotides as branching points and DNA or LNA as triplex forming branch
AU - Sørensen, M.D.
AU - Meldgaard, M.
AU - Rajwanshi, V.K.
AU - Wengel, J.
PY - 2000
Y1 - 2000
N2 - Various Y-shaped branched oligonucleotides containing a 2′-O,3′-C-ethylene linked or 2′-O,4′-C-methylene linked bicyclic nucleotide as branching point were synthesized on an automated DNA synthesizer. Thermal denaturation experiments at 260 and 284 nm showed increased thermal stabilities of complexes formed between these Y-shaped oligonucleotides and complementary DNA compared with those formed with the corresponding linear reference. The most significant effect was observed when LNA (locked nucleic acid) monomers were used in the triplex forming branch.
AB - Various Y-shaped branched oligonucleotides containing a 2′-O,3′-C-ethylene linked or 2′-O,4′-C-methylene linked bicyclic nucleotide as branching point were synthesized on an automated DNA synthesizer. Thermal denaturation experiments at 260 and 284 nm showed increased thermal stabilities of complexes formed between these Y-shaped oligonucleotides and complementary DNA compared with those formed with the corresponding linear reference. The most significant effect was observed when LNA (locked nucleic acid) monomers were used in the triplex forming branch.
U2 - 10.1016/S0960-894X(00)00362-0
DO - 10.1016/S0960-894X(00)00362-0
M3 - Journal article
SN - 0960-894X
VL - 10
SP - 1853
EP - 1856
JO - Bioorganic & Medicinal Chemistry Letters
JF - Bioorganic & Medicinal Chemistry Letters
IS - 16
ER -