Brain lipidomics and neurodevelopmental outcomes in intrauterine growth restricted piglets fed dairy or vegetable fat diets

Nicole L. Henriksen, Karina S. Asmussen, Xiaoyu Pan, Ping Ping Jiang, Yuki Mori, Line I. Christiansen, Richard R. Sprenger, Christer S. Ejsing, Stanislava Pankratova, Thomas Thymann*

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Abstract

Breast milk has neurodevelopmental advantages compared to infant formula, especially in low-birth-weight infants, which may in part relate to the fat source. This study compared neurodevelopmental outcomes in three-day-old normal birth weight (NBW) and intrauterine growth restricted (IUGR) piglets fed a formula diet with either vegetable oil (VEG) or bovine milk fat sources (MILK) for three weeks in a 2 × 2 factorial design. Behavioural tests, lipidomics, MRI and RNA sequencing analyses of plasma and brain tissue were conducted. The absolute levels of 82% and 11% of lipid molecules were different between dietary groups in plasma and hippocampus, respectively. Of the lipid molecules with differential abundance in the hippocampus, the majority were upregulated in MILK versus VEG, and they mainly belonged to the group of glycerophospholipids. Lower absolute brain weights, absolute grey and white matter volumes and behaviour and motor function scores, and higher relative total brain weights were present in IUGR compared to NBW with minor influence of diet. Cognitive function and cerebellar gene expression profiles were similar for dietary and weight groups, and overall only minor interactive effects between diet and birth weight were observed. Overall, we show that the dietary fat source influences the plasma and to a lesser degree the hippocampal lipidome and is unable to improve on IUGR-induced brain structural and functional impairments.

OriginalsprogEngelsk
Artikelnummer3303
TidsskriftScientific Reports
Vol/bind12
Antal sider10
ISSN2045-2322
DOI
StatusUdgivet - 28. feb. 2022

Bibliografisk note

Funding Information:
The authors would like to thank Britta Karlsson, Jane Povlsen and Nikoline Johannson from the Section for Comparative Pediatrics and Nutrition at the University of Copenhagen for their assistance with animal care. Charlotte Amdi Williams is acknowledged for her contribution to funding acquisition and on-farm selection of animals. This study was funded by the Danish Dairy Research Foundation, the VILLUM Center for Bioanalytical Sciences (VKR023179) as well as the Lundbeck Foundation (R54-A5858).

Funding Information:
The authors would like to thank Britta Karlsson, Jane Povlsen and Nikoline Johannson from the Section for Comparative Pediatrics and Nutrition at the University of Copenhagen for their assistance with animal care. Charlotte Amdi Williams is acknowledged for her contribution to funding acquisition and on-farm selection of animals. This study was funded by the Danish Dairy Research Foundation, the VILLUM Center for Bioanalytical Sciences (VKR023179) as well as the Lundbeck Foundation (R54-A5858).

Publisher Copyright:
© 2022, The Author(s).

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