Bone turnover markers are differentially affected by pre-analytical handling

G. L. Christensen, J. R. Halgreen, M. Milenkovski, A. Köse, N. Quardon, N. R. Jørgensen*

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til tidsskriftLetterForskningpeer review

Resumé

Summary: Given that bone turnover markers are often shipped to central laboratories, it is essential to be aware of factors that will affect stability. We have evaluated how sample type, time before separation of blood samples, and time between separation and analysis affect the stability of four bone turnover markers. Introduction: Bone turnover markers are often shipped to central laboratories for analysis, which require knowledge of the stability of the markers of interest in different sample materials. The aim of the current study was to evaluate how time before separation of blood samples and time between separation and analysis affect the stability of four bone turnover markers in serum and plasma samples. Methods: Serum, EDTA, and Lithium heparin (LiHep) plasma samples from seven osteoporosis patients and three healthy controls were collected and stored at room temperature for up to 72 h before separation and analysis. After separation, samples were stored at room temperature for up to 72 h and re-analyzed. The bone turnover markers N-terminal pro-collagen type 1 extension pro-peptide (P1NP), bone-specific alkaline phosphatase (BAP), C-terminal teleopeptide cross links of collagen type 1 (CTX), and osteocalcin (OC) were analyzed using the automated iSYS IDS platform. Results: P1NP and BAP were stable in both plasma and serum for 72 h before centrifugation. CTX levels were higher in EDTA plasma at all time points compared to LiHep plasma and serum. The use of EDTA plasma prolonged the stability of CTX as compared to LiHep plasma and serum. Osteocalcin showed high tendency to degrade in all sample types and concentrations were significantly lower after 24 h of storage. Conclusions: For the bone turnover markers P1NP and BAP, the use of both plasma and serum is recommended. Samples for CTX analysis should be taken as EDTA plasma. Samples for osteocalcin analysis can be taken in either type of plasma or serum, but should be analyzed within 3 h or preserved at − 18 °C.

OriginalsprogEngelsk
TidsskriftOsteoporosis International
Vol/bind30
Udgave nummer5
Sider (fra-til)1137-1141
ISSN0937-941X
DOI
StatusUdgivet - 1. maj 2019

Fingeraftryk

Edetic Acid
Osteocalcin
Serum
Lithium
Alkaline Phosphatase
Collagen Type I
Centrifugation

Citer dette

Christensen, G. L. ; Halgreen, J. R. ; Milenkovski, M. ; Köse, A. ; Quardon, N. ; Jørgensen, N. R. / Bone turnover markers are differentially affected by pre-analytical handling. I: Osteoporosis International. 2019 ; Bind 30, Nr. 5. s. 1137-1141.
@article{086da47b14874e2980b5ce690b5aeb88,
title = "Bone turnover markers are differentially affected by pre-analytical handling",
abstract = "Summary: Given that bone turnover markers are often shipped to central laboratories, it is essential to be aware of factors that will affect stability. We have evaluated how sample type, time before separation of blood samples, and time between separation and analysis affect the stability of four bone turnover markers. Introduction: Bone turnover markers are often shipped to central laboratories for analysis, which require knowledge of the stability of the markers of interest in different sample materials. The aim of the current study was to evaluate how time before separation of blood samples and time between separation and analysis affect the stability of four bone turnover markers in serum and plasma samples. Methods: Serum, EDTA, and Lithium heparin (LiHep) plasma samples from seven osteoporosis patients and three healthy controls were collected and stored at room temperature for up to 72 h before separation and analysis. After separation, samples were stored at room temperature for up to 72 h and re-analyzed. The bone turnover markers N-terminal pro-collagen type 1 extension pro-peptide (P1NP), bone-specific alkaline phosphatase (BAP), C-terminal teleopeptide cross links of collagen type 1 (CTX), and osteocalcin (OC) were analyzed using the automated iSYS IDS platform. Results: P1NP and BAP were stable in both plasma and serum for 72 h before centrifugation. CTX levels were higher in EDTA plasma at all time points compared to LiHep plasma and serum. The use of EDTA plasma prolonged the stability of CTX as compared to LiHep plasma and serum. Osteocalcin showed high tendency to degrade in all sample types and concentrations were significantly lower after 24 h of storage. Conclusions: For the bone turnover markers P1NP and BAP, the use of both plasma and serum is recommended. Samples for CTX analysis should be taken as EDTA plasma. Samples for osteocalcin analysis can be taken in either type of plasma or serum, but should be analyzed within 3 h or preserved at − 18 °C.",
keywords = "BAP, Bone turnover markers, CTX, Osteocalcin, P1NP, Pre-analytical, Stability",
author = "Christensen, {G. L.} and Halgreen, {J. R.} and M. Milenkovski and A. K{\"o}se and N. Quardon and J{\o}rgensen, {N. R.}",
year = "2019",
month = "5",
day = "1",
doi = "10.1007/s00198-019-04837-7",
language = "English",
volume = "30",
pages = "1137--1141",
journal = "Osteoporosis International",
issn = "0937-941X",
publisher = "Springer",
number = "5",

}

Christensen, GL, Halgreen, JR, Milenkovski, M, Köse, A, Quardon, N & Jørgensen, NR 2019, 'Bone turnover markers are differentially affected by pre-analytical handling', Osteoporosis International, bind 30, nr. 5, s. 1137-1141. https://doi.org/10.1007/s00198-019-04837-7

Bone turnover markers are differentially affected by pre-analytical handling. / Christensen, G. L.; Halgreen, J. R.; Milenkovski, M.; Köse, A.; Quardon, N.; Jørgensen, N. R.

I: Osteoporosis International, Bind 30, Nr. 5, 01.05.2019, s. 1137-1141.

Publikation: Bidrag til tidsskriftLetterForskningpeer review

TY - JOUR

T1 - Bone turnover markers are differentially affected by pre-analytical handling

AU - Christensen, G. L.

AU - Halgreen, J. R.

AU - Milenkovski, M.

AU - Köse, A.

AU - Quardon, N.

AU - Jørgensen, N. R.

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Summary: Given that bone turnover markers are often shipped to central laboratories, it is essential to be aware of factors that will affect stability. We have evaluated how sample type, time before separation of blood samples, and time between separation and analysis affect the stability of four bone turnover markers. Introduction: Bone turnover markers are often shipped to central laboratories for analysis, which require knowledge of the stability of the markers of interest in different sample materials. The aim of the current study was to evaluate how time before separation of blood samples and time between separation and analysis affect the stability of four bone turnover markers in serum and plasma samples. Methods: Serum, EDTA, and Lithium heparin (LiHep) plasma samples from seven osteoporosis patients and three healthy controls were collected and stored at room temperature for up to 72 h before separation and analysis. After separation, samples were stored at room temperature for up to 72 h and re-analyzed. The bone turnover markers N-terminal pro-collagen type 1 extension pro-peptide (P1NP), bone-specific alkaline phosphatase (BAP), C-terminal teleopeptide cross links of collagen type 1 (CTX), and osteocalcin (OC) were analyzed using the automated iSYS IDS platform. Results: P1NP and BAP were stable in both plasma and serum for 72 h before centrifugation. CTX levels were higher in EDTA plasma at all time points compared to LiHep plasma and serum. The use of EDTA plasma prolonged the stability of CTX as compared to LiHep plasma and serum. Osteocalcin showed high tendency to degrade in all sample types and concentrations were significantly lower after 24 h of storage. Conclusions: For the bone turnover markers P1NP and BAP, the use of both plasma and serum is recommended. Samples for CTX analysis should be taken as EDTA plasma. Samples for osteocalcin analysis can be taken in either type of plasma or serum, but should be analyzed within 3 h or preserved at − 18 °C.

AB - Summary: Given that bone turnover markers are often shipped to central laboratories, it is essential to be aware of factors that will affect stability. We have evaluated how sample type, time before separation of blood samples, and time between separation and analysis affect the stability of four bone turnover markers. Introduction: Bone turnover markers are often shipped to central laboratories for analysis, which require knowledge of the stability of the markers of interest in different sample materials. The aim of the current study was to evaluate how time before separation of blood samples and time between separation and analysis affect the stability of four bone turnover markers in serum and plasma samples. Methods: Serum, EDTA, and Lithium heparin (LiHep) plasma samples from seven osteoporosis patients and three healthy controls were collected and stored at room temperature for up to 72 h before separation and analysis. After separation, samples were stored at room temperature for up to 72 h and re-analyzed. The bone turnover markers N-terminal pro-collagen type 1 extension pro-peptide (P1NP), bone-specific alkaline phosphatase (BAP), C-terminal teleopeptide cross links of collagen type 1 (CTX), and osteocalcin (OC) were analyzed using the automated iSYS IDS platform. Results: P1NP and BAP were stable in both plasma and serum for 72 h before centrifugation. CTX levels were higher in EDTA plasma at all time points compared to LiHep plasma and serum. The use of EDTA plasma prolonged the stability of CTX as compared to LiHep plasma and serum. Osteocalcin showed high tendency to degrade in all sample types and concentrations were significantly lower after 24 h of storage. Conclusions: For the bone turnover markers P1NP and BAP, the use of both plasma and serum is recommended. Samples for CTX analysis should be taken as EDTA plasma. Samples for osteocalcin analysis can be taken in either type of plasma or serum, but should be analyzed within 3 h or preserved at − 18 °C.

KW - BAP

KW - Bone turnover markers

KW - CTX

KW - Osteocalcin

KW - P1NP

KW - Pre-analytical

KW - Stability

U2 - 10.1007/s00198-019-04837-7

DO - 10.1007/s00198-019-04837-7

M3 - Letter

VL - 30

SP - 1137

EP - 1141

JO - Osteoporosis International

JF - Osteoporosis International

SN - 0937-941X

IS - 5

ER -