BMP2 and TGF-β Cooperate Differently during Synovial-Derived Stem-Cell Chondrogenesis in a Dexamethasone-Dependent Manner

Nikolas J Kovermann, Valentina Basoli, Elena Della Bella, Mauro Alini, Christoph Lischer, Hagen Schmal, Eva Johanna Kubosch, Martin J Stoddart

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Abstrakt

Recent studies highlighting mesenchymal stem cell (MSC) epigenetic memory suggest that a different differentiation medium may be required depending on the tissue of origin. As synovial-derived stem cells (SDSCs) attract interest we aimed to investigate the influence of TGF-β1, BMP-2 and dexamethasone on SDSC chondrogenesis in vitro. We demonstrate that dexamethasone-free medium led to enhanced chondrogenic differentiation at both the mRNA and matrix level. The greatest COL2A1/COL10A1 ratio was detected in cells exposed to a combination medium containing 10 ng/mL BMP-2 and 1 ng/mL TGF-β1 in the absence of dexamethasone, and this was reflected in the total amount of glycosaminoglycans produced. In summary, dexamethasone-free medium containing BMP-2 and TGF-β1 may be the most suitable when using SDSCs for cartilage tissue regeneration.

OriginalsprogEngelsk
Artikelnummer636
TidsskriftCells
Vol/bind8
Udgave nummer6
Antal sider17
ISSN2073-4409
DOI
StatusUdgivet - 25. jun. 2019

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