Biphasic effect of relaxin, inhibitable by a collagenase inhibitor, on the strength of human fetal membranes

Ida Vogel, Astrid Petersen, Lone Kjeld Petersen, Rikke Bek Helmig, Hans Oxlund, Niels Uldbjerg

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

BACKGROUND: Relaxin has been proposed as a hormone involved in the collagen remodeling of the utero-placental unit.

MATERIALS AND METHODS: Human fetal membrane explants were incubated with H1 or H2 relaxin for 48 hours and stretched until rupture in a materials testing machine. Co-incubation with a synthetic collagenase inhibitor was performed in order to examine whether the effects of relaxin could be inhibited. The effects on hydroxyproline and histology were evaluated.

RESULTS: Biomechanical testing showed that H2 relaxin induced a biphasic weakening of human fetal membranes, an effect that was abolished after co-incubation with a collagenase inhibitor. H1 relaxin produced no significant effects on the biomechanical properties. The effects of H2 relaxin on the biomechanical properties were, however, not followed by changes in the hydroxyproline concentration or the histology.

CONCLUSION: H2 relaxin had an effect on human fetal membranes and this effect may be mediated through collagenases.

OriginalsprogEngelsk
TidsskriftIn Vivo
Vol/bind18
Udgave nummer5
Sider (fra-til)581-4
Antal sider4
ISSN0258-851X
StatusUdgivet - 5. nov. 2004

Fingeraftryk

Relaxin
Matrix Metalloproteinase Inhibitors
Membranes
Histology
Hydroxyproline
Materials Testing
Materials testing
Collagenases
Rupture
Collagen
Hormones
Testing

Citer dette

Vogel, I., Petersen, A., Petersen, L. K., Helmig, R. B., Oxlund, H., & Uldbjerg, N. (2004). Biphasic effect of relaxin, inhibitable by a collagenase inhibitor, on the strength of human fetal membranes. In Vivo, 18(5), 581-4.
Vogel, Ida ; Petersen, Astrid ; Petersen, Lone Kjeld ; Helmig, Rikke Bek ; Oxlund, Hans ; Uldbjerg, Niels. / Biphasic effect of relaxin, inhibitable by a collagenase inhibitor, on the strength of human fetal membranes. I: In Vivo. 2004 ; Bind 18, Nr. 5. s. 581-4.
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title = "Biphasic effect of relaxin, inhibitable by a collagenase inhibitor, on the strength of human fetal membranes",
abstract = "BACKGROUND: Relaxin has been proposed as a hormone involved in the collagen remodeling of the utero-placental unit.MATERIALS AND METHODS: Human fetal membrane explants were incubated with H1 or H2 relaxin for 48 hours and stretched until rupture in a materials testing machine. Co-incubation with a synthetic collagenase inhibitor was performed in order to examine whether the effects of relaxin could be inhibited. The effects on hydroxyproline and histology were evaluated.RESULTS: Biomechanical testing showed that H2 relaxin induced a biphasic weakening of human fetal membranes, an effect that was abolished after co-incubation with a collagenase inhibitor. H1 relaxin produced no significant effects on the biomechanical properties. The effects of H2 relaxin on the biomechanical properties were, however, not followed by changes in the hydroxyproline concentration or the histology.CONCLUSION: H2 relaxin had an effect on human fetal membranes and this effect may be mediated through collagenases.",
keywords = "Dose-Response Relationship, Drug, Enzyme Inhibitors/pharmacology, Extraembryonic Membranes/anatomy & histology, Humans, Hydroxyproline/metabolism, Matrix Metalloproteinase Inhibitors, Relaxin/pharmacology, Tensile Strength/drug effects",
author = "Ida Vogel and Astrid Petersen and Petersen, {Lone Kjeld} and Helmig, {Rikke Bek} and Hans Oxlund and Niels Uldbjerg",
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Vogel, I, Petersen, A, Petersen, LK, Helmig, RB, Oxlund, H & Uldbjerg, N 2004, 'Biphasic effect of relaxin, inhibitable by a collagenase inhibitor, on the strength of human fetal membranes', In Vivo, bind 18, nr. 5, s. 581-4.

Biphasic effect of relaxin, inhibitable by a collagenase inhibitor, on the strength of human fetal membranes. / Vogel, Ida; Petersen, Astrid; Petersen, Lone Kjeld; Helmig, Rikke Bek; Oxlund, Hans; Uldbjerg, Niels.

I: In Vivo, Bind 18, Nr. 5, 05.11.2004, s. 581-4.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Biphasic effect of relaxin, inhibitable by a collagenase inhibitor, on the strength of human fetal membranes

AU - Vogel, Ida

AU - Petersen, Astrid

AU - Petersen, Lone Kjeld

AU - Helmig, Rikke Bek

AU - Oxlund, Hans

AU - Uldbjerg, Niels

PY - 2004/11/5

Y1 - 2004/11/5

N2 - BACKGROUND: Relaxin has been proposed as a hormone involved in the collagen remodeling of the utero-placental unit.MATERIALS AND METHODS: Human fetal membrane explants were incubated with H1 or H2 relaxin for 48 hours and stretched until rupture in a materials testing machine. Co-incubation with a synthetic collagenase inhibitor was performed in order to examine whether the effects of relaxin could be inhibited. The effects on hydroxyproline and histology were evaluated.RESULTS: Biomechanical testing showed that H2 relaxin induced a biphasic weakening of human fetal membranes, an effect that was abolished after co-incubation with a collagenase inhibitor. H1 relaxin produced no significant effects on the biomechanical properties. The effects of H2 relaxin on the biomechanical properties were, however, not followed by changes in the hydroxyproline concentration or the histology.CONCLUSION: H2 relaxin had an effect on human fetal membranes and this effect may be mediated through collagenases.

AB - BACKGROUND: Relaxin has been proposed as a hormone involved in the collagen remodeling of the utero-placental unit.MATERIALS AND METHODS: Human fetal membrane explants were incubated with H1 or H2 relaxin for 48 hours and stretched until rupture in a materials testing machine. Co-incubation with a synthetic collagenase inhibitor was performed in order to examine whether the effects of relaxin could be inhibited. The effects on hydroxyproline and histology were evaluated.RESULTS: Biomechanical testing showed that H2 relaxin induced a biphasic weakening of human fetal membranes, an effect that was abolished after co-incubation with a collagenase inhibitor. H1 relaxin produced no significant effects on the biomechanical properties. The effects of H2 relaxin on the biomechanical properties were, however, not followed by changes in the hydroxyproline concentration or the histology.CONCLUSION: H2 relaxin had an effect on human fetal membranes and this effect may be mediated through collagenases.

KW - Dose-Response Relationship, Drug

KW - Enzyme Inhibitors/pharmacology

KW - Extraembryonic Membranes/anatomy & histology

KW - Humans

KW - Hydroxyproline/metabolism

KW - Matrix Metalloproteinase Inhibitors

KW - Relaxin/pharmacology

KW - Tensile Strength/drug effects

M3 - Journal article

VL - 18

SP - 581

EP - 584

JO - In Vivo

JF - In Vivo

SN - 0258-851X

IS - 5

ER -