TY - JOUR
T1 - Bioconversion of wastewater to single cell protein by methanotrophic bacteria
AU - Zha, Xiao
AU - Tsapekos, Panagiotis
AU - Zhu, Xinyu
AU - Khoshnevisan, Benyamin
AU - Lu, Xiwu
AU - Angelidaki, Irini
N1 - Funding Information:
The authors thank the FUBAF project financed by the Danish EPA - MUDP (J.nr. Mst-11700508). Xiao Zha would like to thank for the financial support from China Scholarship Council.
PY - 2021/1
Y1 - 2021/1
N2 - Single cell protein (SCP) provides an alternative protein source to partially replace the conventional agricultural resources and support the increased nutritional needs. Inexpensive feeding source is one of the key limiting factors for the expansion of SCP production. The present study examined the valorization of biogas derived from the anaerobic digestion (AD) of sewage sludge and the discarded effluent as nutrients source to produce SCP using methanotrophic bacteria. Results indicated that the mixed methanotrophic culture can grow well on the pasteurized AD supernatant and biogas, succeeding in promising dry weight (DW) yield (0.66 ± 0.01 g-DW/g-CH4 and 11.54 ± 0.12 g-DW/g-NH4+). Methylomonas (56.26%) and Methylophilus (24.60%) spp. were the two main representatives of the mixed culture. The produced dried biomass had a protein content higher than 41% w/w, including essential amino acids like histidine, valine, phenylalanine, isoleucine, leucine, threonine and lysine. The cultivated SCP shows potential utilization as protein source for animal diets.
AB - Single cell protein (SCP) provides an alternative protein source to partially replace the conventional agricultural resources and support the increased nutritional needs. Inexpensive feeding source is one of the key limiting factors for the expansion of SCP production. The present study examined the valorization of biogas derived from the anaerobic digestion (AD) of sewage sludge and the discarded effluent as nutrients source to produce SCP using methanotrophic bacteria. Results indicated that the mixed methanotrophic culture can grow well on the pasteurized AD supernatant and biogas, succeeding in promising dry weight (DW) yield (0.66 ± 0.01 g-DW/g-CH4 and 11.54 ± 0.12 g-DW/g-NH4+). Methylomonas (56.26%) and Methylophilus (24.60%) spp. were the two main representatives of the mixed culture. The produced dried biomass had a protein content higher than 41% w/w, including essential amino acids like histidine, valine, phenylalanine, isoleucine, leucine, threonine and lysine. The cultivated SCP shows potential utilization as protein source for animal diets.
KW - 16S rRNA
KW - Digested sludge
KW - Methanotrophic bacteria
KW - Single cell protein
KW - Wastewater
U2 - 10.1016/j.biortech.2020.124351
DO - 10.1016/j.biortech.2020.124351
M3 - Journal article
C2 - 33161316
AN - SCOPUS:85095455036
SN - 0960-8524
VL - 320
JO - Bioresource Technology
JF - Bioresource Technology
IS - Pt. A
M1 - 124351
ER -