Biochemical and Bioimaging Evidence of Cholesterol in Acquired Cholesteatoma

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

OBJECTIVES: To quantify the barrier sterols and image the lipid structures in the matrix of acquired cholesteatoma and compare the distribution with that found in stratum corneum from normal skin, with the goal to resolve their potential influence on cholesteatoma growth.

METHODS: High-performance thin-layer chromatography (HPTLC) was used to achieve a quantitative biochemical determination of the sterols. The intercellular lipids were visualized by Coherent Anti-Stokes Raman scattering (CARS) microscopy, which enables label-free imaging of the lipids in intact tissue samples.

RESULTS: The results show that the total lipid content of the cholesteatoma matrix is similar to that of stratum corneum from skin and that the cholesteatoma matrix unquestionably contains cholesterol. The cholesterol content in the cholesteatoma matrix is increased by over 30% (w/w dry weight) compared to the control. The cholesterol sulfate content is below 1% of the total lipids in both the cholesteatoma and the control. Cholesterol ester was reduced by over 30% when compared to the control.

CONCLUSIONS: The content of cholesterol in the cholesteatoma matrix is significantly different from that in stratum corneum from skin, and we confirm that the main structure of the cholesteatoma resembles very thick stratum corneum.

OriginalsprogEngelsk
TidsskriftAnnals of Otology, Rhinology and Laryngology
Vol/bind125
Udgave nummer8
Sider (fra-til)627-633
ISSN0003-4894
DOI
StatusUdgivet - 15. apr. 2016

Fingeraftryk

Cholesteatoma
Lipids
Skin
Cholesterol Esters
Thin Layer Chromatography
Microscopy
Weights and Measures
Growth

Citer dette

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title = "Biochemical and Bioimaging Evidence of Cholesterol in Acquired Cholesteatoma",
abstract = "OBJECTIVES: To quantify the barrier sterols and image the lipid structures in the matrix of acquired cholesteatoma and compare the distribution with that found in stratum corneum from normal skin, with the goal to resolve their potential influence on cholesteatoma growth.METHODS: High-performance thin-layer chromatography (HPTLC) was used to achieve a quantitative biochemical determination of the sterols. The intercellular lipids were visualized by Coherent Anti-Stokes Raman scattering (CARS) microscopy, which enables label-free imaging of the lipids in intact tissue samples.RESULTS: The results show that the total lipid content of the cholesteatoma matrix is similar to that of stratum corneum from skin and that the cholesteatoma matrix unquestionably contains cholesterol. The cholesterol content in the cholesteatoma matrix is increased by over 30{\%} (w/w dry weight) compared to the control. The cholesterol sulfate content is below 1{\%} of the total lipids in both the cholesteatoma and the control. Cholesterol ester was reduced by over 30{\%} when compared to the control.CONCLUSIONS: The content of cholesterol in the cholesteatoma matrix is significantly different from that in stratum corneum from skin, and we confirm that the main structure of the cholesteatoma resembles very thick stratum corneum.",
author = "Bjarne Thorsted and Maria Bloksgaard and Alexandra Groza and Peter Schousboe and F{\ae}rgeman, {Nils J.} and S{\o}rensen, {Jens Ahm} and Viggo Svane-Knudsen and Brewer, {Jonathan R}",
note = "Published online before print April 15, 2016.",
year = "2016",
month = "4",
day = "15",
doi = "10.1177/0003489416642784",
language = "English",
volume = "125",
pages = "627--633",
journal = "Annals of Otology, Rhinology and Laryngology",
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Biochemical and Bioimaging Evidence of Cholesterol in Acquired Cholesteatoma. / Thorsted, Bjarne; Bloksgaard, Maria; Groza, Alexandra; Schousboe, Peter; Færgeman, Nils J.; Sørensen, Jens Ahm; Svane-Knudsen, Viggo; Brewer, Jonathan R.

I: Annals of Otology, Rhinology and Laryngology, Bind 125, Nr. 8, 15.04.2016, s. 627-633.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Biochemical and Bioimaging Evidence of Cholesterol in Acquired Cholesteatoma

AU - Thorsted, Bjarne

AU - Bloksgaard, Maria

AU - Groza, Alexandra

AU - Schousboe, Peter

AU - Færgeman, Nils J.

AU - Sørensen, Jens Ahm

AU - Svane-Knudsen, Viggo

AU - Brewer, Jonathan R

N1 - Published online before print April 15, 2016.

PY - 2016/4/15

Y1 - 2016/4/15

N2 - OBJECTIVES: To quantify the barrier sterols and image the lipid structures in the matrix of acquired cholesteatoma and compare the distribution with that found in stratum corneum from normal skin, with the goal to resolve their potential influence on cholesteatoma growth.METHODS: High-performance thin-layer chromatography (HPTLC) was used to achieve a quantitative biochemical determination of the sterols. The intercellular lipids were visualized by Coherent Anti-Stokes Raman scattering (CARS) microscopy, which enables label-free imaging of the lipids in intact tissue samples.RESULTS: The results show that the total lipid content of the cholesteatoma matrix is similar to that of stratum corneum from skin and that the cholesteatoma matrix unquestionably contains cholesterol. The cholesterol content in the cholesteatoma matrix is increased by over 30% (w/w dry weight) compared to the control. The cholesterol sulfate content is below 1% of the total lipids in both the cholesteatoma and the control. Cholesterol ester was reduced by over 30% when compared to the control.CONCLUSIONS: The content of cholesterol in the cholesteatoma matrix is significantly different from that in stratum corneum from skin, and we confirm that the main structure of the cholesteatoma resembles very thick stratum corneum.

AB - OBJECTIVES: To quantify the barrier sterols and image the lipid structures in the matrix of acquired cholesteatoma and compare the distribution with that found in stratum corneum from normal skin, with the goal to resolve their potential influence on cholesteatoma growth.METHODS: High-performance thin-layer chromatography (HPTLC) was used to achieve a quantitative biochemical determination of the sterols. The intercellular lipids were visualized by Coherent Anti-Stokes Raman scattering (CARS) microscopy, which enables label-free imaging of the lipids in intact tissue samples.RESULTS: The results show that the total lipid content of the cholesteatoma matrix is similar to that of stratum corneum from skin and that the cholesteatoma matrix unquestionably contains cholesterol. The cholesterol content in the cholesteatoma matrix is increased by over 30% (w/w dry weight) compared to the control. The cholesterol sulfate content is below 1% of the total lipids in both the cholesteatoma and the control. Cholesterol ester was reduced by over 30% when compared to the control.CONCLUSIONS: The content of cholesterol in the cholesteatoma matrix is significantly different from that in stratum corneum from skin, and we confirm that the main structure of the cholesteatoma resembles very thick stratum corneum.

U2 - 10.1177/0003489416642784

DO - 10.1177/0003489416642784

M3 - Journal article

VL - 125

SP - 627

EP - 633

JO - Annals of Otology, Rhinology and Laryngology

JF - Annals of Otology, Rhinology and Laryngology

SN - 0003-4894

IS - 8

ER -