Bioactive Nano-fibrous Scaffold for Vascularized Craniofacial Bone Regeneration

Rahul Damodaran Prabha, David Christian Evar Kraft, Linda Harkness, Birte Melsen, Harikrishna Varma, Prabha D Nair, Jorgen Kjems, Mousthapha Kassem

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Resumé

There has been a growing demand for bone grafts for correction of bone defects in complicated fractures or tumours in the craniofacial region. Soft flexible membrane like material that could be inserted into defect by less invasive approaches; promote osteoconductivity and act as a barrier to soft tissue in growth while promoting bone formation is an attractive option for this region. Electrospinning has recently emerged as one of the most promising techniques for fabrication of extracellular matrix such as nano-fibrous scaffolds that can serve as a template for bone formation. To overcome the limitation of cell penetration of electrospun scaffolds and improve on its osteoconductive nature, in this study, we fabricated a novel electrospun composite scaffold of polyvinyl alcohol (PVA)-poly (ε) caprolactone (PCL)-Hydroxyapatite based bioceramic (HAB), namely, PVA-PCL-HAB. The scaffold prepared by dual electrospinning of PVA and PCL with HAB overcomes reduced cell attachment associated with hydrophobic PCL by combination with a hydrophilic PVA and the HAB can contribute to enhance osteoconductivity. We characterized the physicochemical and biocompatibility properties of the new scaffold material. Our results indicate PVA-PCL-HAB scaffolds support attachment and growth of stromal stem cells; [human bone marrow skeletal (mesenchymal) stem cells and dental pulp stem cells]. In addition, the scaffold supported in vitro osteogenic differentiation and in vivo vascularized bone formation. Thus, PVA-PCL-HAB scaffold is a suitable potential material for therapeutic bone regeneration in dentistry and orthopaedics.

OriginalsprogEngelsk
TidsskriftJournal of Tissue Engineering and Regenerative Medicine
Vol/bind12
Udgave nummer3
Sider (fra-til)e1537-e1548
ISSN1932-6254
DOI
StatusUdgivet - mar. 2018

Fingeraftryk

Polyvinyl Alcohol
Bioceramics
Durapatite
Scaffolds
Polyvinyl alcohols
Hydroxyapatite
Bone
Osteogenesis
Stem cells
Electrospinning
Dental Pulp
Dentistry
Defects
Stromal Cells
Growth
Mesenchymal Stromal Cells
Orthopedics
Scaffolds (biology)
polycaprolactone
Biocompatibility

Citer dette

Prabha, Rahul Damodaran ; Kraft, David Christian Evar ; Harkness, Linda ; Melsen, Birte ; Varma, Harikrishna ; Nair, Prabha D ; Kjems, Jorgen ; Kassem, Mousthapha. / Bioactive Nano-fibrous Scaffold for Vascularized Craniofacial Bone Regeneration. I: Journal of Tissue Engineering and Regenerative Medicine. 2018 ; Bind 12, Nr. 3. s. e1537-e1548.
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title = "Bioactive Nano-fibrous Scaffold for Vascularized Craniofacial Bone Regeneration",
abstract = "There has been a growing demand for bone grafts for correction of bone defects in complicated fractures or tumours in the craniofacial region. Soft flexible membrane like material that could be inserted into defect by less invasive approaches; promote osteoconductivity and act as a barrier to soft tissue in growth while promoting bone formation is an attractive option for this region. Electrospinning has recently emerged as one of the most promising techniques for fabrication of extracellular matrix such as nano-fibrous scaffolds that can serve as a template for bone formation. To overcome the limitation of cell penetration of electrospun scaffolds and improve on its osteoconductive nature, in this study, we fabricated a novel electrospun composite scaffold of polyvinyl alcohol (PVA)-poly (ε) caprolactone (PCL)-Hydroxyapatite based bioceramic (HAB), namely, PVA-PCL-HAB. The scaffold prepared by dual electrospinning of PVA and PCL with HAB overcomes reduced cell attachment associated with hydrophobic PCL by combination with a hydrophilic PVA and the HAB can contribute to enhance osteoconductivity. We characterized the physicochemical and biocompatibility properties of the new scaffold material. Our results indicate PVA-PCL-HAB scaffolds support attachment and growth of stromal stem cells; [human bone marrow skeletal (mesenchymal) stem cells and dental pulp stem cells]. In addition, the scaffold supported in vitro osteogenic differentiation and in vivo vascularized bone formation. Thus, PVA-PCL-HAB scaffold is a suitable potential material for therapeutic bone regeneration in dentistry and orthopaedics.",
keywords = "bioceramics, bone, craniofacial, electrospinning, scaffold, stem cells",
author = "Prabha, {Rahul Damodaran} and Kraft, {David Christian Evar} and Linda Harkness and Birte Melsen and Harikrishna Varma and Nair, {Prabha D} and Jorgen Kjems and Mousthapha Kassem",
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year = "2018",
month = "3",
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Prabha, RD, Kraft, DCE, Harkness, L, Melsen, B, Varma, H, Nair, PD, Kjems, J & Kassem, M 2018, 'Bioactive Nano-fibrous Scaffold for Vascularized Craniofacial Bone Regeneration', Journal of Tissue Engineering and Regenerative Medicine, bind 12, nr. 3, s. e1537-e1548. https://doi.org/10.1002/term.2579

Bioactive Nano-fibrous Scaffold for Vascularized Craniofacial Bone Regeneration. / Prabha, Rahul Damodaran; Kraft, David Christian Evar; Harkness, Linda; Melsen, Birte; Varma, Harikrishna; Nair, Prabha D; Kjems, Jorgen; Kassem, Mousthapha.

I: Journal of Tissue Engineering and Regenerative Medicine, Bind 12, Nr. 3, 03.2018, s. e1537-e1548.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Bioactive Nano-fibrous Scaffold for Vascularized Craniofacial Bone Regeneration

AU - Prabha, Rahul Damodaran

AU - Kraft, David Christian Evar

AU - Harkness, Linda

AU - Melsen, Birte

AU - Varma, Harikrishna

AU - Nair, Prabha D

AU - Kjems, Jorgen

AU - Kassem, Mousthapha

N1 - This article is protected by copyright. All rights reserved.

PY - 2018/3

Y1 - 2018/3

N2 - There has been a growing demand for bone grafts for correction of bone defects in complicated fractures or tumours in the craniofacial region. Soft flexible membrane like material that could be inserted into defect by less invasive approaches; promote osteoconductivity and act as a barrier to soft tissue in growth while promoting bone formation is an attractive option for this region. Electrospinning has recently emerged as one of the most promising techniques for fabrication of extracellular matrix such as nano-fibrous scaffolds that can serve as a template for bone formation. To overcome the limitation of cell penetration of electrospun scaffolds and improve on its osteoconductive nature, in this study, we fabricated a novel electrospun composite scaffold of polyvinyl alcohol (PVA)-poly (ε) caprolactone (PCL)-Hydroxyapatite based bioceramic (HAB), namely, PVA-PCL-HAB. The scaffold prepared by dual electrospinning of PVA and PCL with HAB overcomes reduced cell attachment associated with hydrophobic PCL by combination with a hydrophilic PVA and the HAB can contribute to enhance osteoconductivity. We characterized the physicochemical and biocompatibility properties of the new scaffold material. Our results indicate PVA-PCL-HAB scaffolds support attachment and growth of stromal stem cells; [human bone marrow skeletal (mesenchymal) stem cells and dental pulp stem cells]. In addition, the scaffold supported in vitro osteogenic differentiation and in vivo vascularized bone formation. Thus, PVA-PCL-HAB scaffold is a suitable potential material for therapeutic bone regeneration in dentistry and orthopaedics.

AB - There has been a growing demand for bone grafts for correction of bone defects in complicated fractures or tumours in the craniofacial region. Soft flexible membrane like material that could be inserted into defect by less invasive approaches; promote osteoconductivity and act as a barrier to soft tissue in growth while promoting bone formation is an attractive option for this region. Electrospinning has recently emerged as one of the most promising techniques for fabrication of extracellular matrix such as nano-fibrous scaffolds that can serve as a template for bone formation. To overcome the limitation of cell penetration of electrospun scaffolds and improve on its osteoconductive nature, in this study, we fabricated a novel electrospun composite scaffold of polyvinyl alcohol (PVA)-poly (ε) caprolactone (PCL)-Hydroxyapatite based bioceramic (HAB), namely, PVA-PCL-HAB. The scaffold prepared by dual electrospinning of PVA and PCL with HAB overcomes reduced cell attachment associated with hydrophobic PCL by combination with a hydrophilic PVA and the HAB can contribute to enhance osteoconductivity. We characterized the physicochemical and biocompatibility properties of the new scaffold material. Our results indicate PVA-PCL-HAB scaffolds support attachment and growth of stromal stem cells; [human bone marrow skeletal (mesenchymal) stem cells and dental pulp stem cells]. In addition, the scaffold supported in vitro osteogenic differentiation and in vivo vascularized bone formation. Thus, PVA-PCL-HAB scaffold is a suitable potential material for therapeutic bone regeneration in dentistry and orthopaedics.

KW - bioceramics

KW - bone

KW - craniofacial

KW - electrospinning

KW - scaffold

KW - stem cells

U2 - 10.1002/term.2579

DO - 10.1002/term.2579

M3 - Journal article

C2 - 28967188

VL - 12

SP - e1537-e1548

JO - Journal of Tissue Engineering and Regenerative Medicine

JF - Journal of Tissue Engineering and Regenerative Medicine

SN - 1932-6254

IS - 3

ER -