BASP1 is a transcriptional cosuppressor for the Wilms' tumor suppressor protein WT1.

Brian Carpenter, Kathryn J Hill, Marika Charalambous, Kate J Wagner, Diya Lahiri, Dominic I James, Jens S Andersen, Valérie Schumacher, Brigitte Royer-Pokora, Matthias Mann, Andrew Ward, Stefan G E Roberts

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Resumé

The Wilms' tumor suppressor protein WT1 is a transcriptional regulator that plays a key role in the development of the kidneys. The transcriptional activation domain of WT1 is subject to regulation by a suppression region within the N terminus of WT1. Using a functional assay, we provide direct evidence that this requires a transcriptional cosuppressor, which we identify as brain acid soluble protein 1 (BASP1). WT1 and BASP1 associate within the nuclei of cells that naturally express both proteins. BASP1 can confer WT1 cosuppressor activity in transfection assays, and elimination of endogenous BASP1 expression augments transcriptional activation by WT1. BASP1 is present in the developing nephron structures of the embryonic kidney and, coincident with that of WT1, its expression is restricted to the highly specialized podocyte cells of the adult kidney. Taken together, our results show that BASP1 is a WT1-associated factor that can regulate WT1 transcriptional activity.
Udgivelsesdato: 2004-Jan
OriginalsprogEngelsk
TidsskriftMolecular and Cellular Biology
Vol/bind24
Udgave nummer2
Sider (fra-til)537-549
ISSN0270-7306
DOI
StatusUdgivet - 2004

Citer dette

Carpenter, B., Hill, K. J., Charalambous, M., Wagner, K. J., Lahiri, D., James, D. I., ... Roberts, S. G. E. (2004). BASP1 is a transcriptional cosuppressor for the Wilms' tumor suppressor protein WT1. Molecular and Cellular Biology, 24(2), 537-549. https://doi.org/10.1128/MCB.24.2.537-549.2004
Carpenter, Brian ; Hill, Kathryn J ; Charalambous, Marika ; Wagner, Kate J ; Lahiri, Diya ; James, Dominic I ; Andersen, Jens S ; Schumacher, Valérie ; Royer-Pokora, Brigitte ; Mann, Matthias ; Ward, Andrew ; Roberts, Stefan G E. / BASP1 is a transcriptional cosuppressor for the Wilms' tumor suppressor protein WT1. I: Molecular and Cellular Biology. 2004 ; Bind 24, Nr. 2. s. 537-549.
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title = "BASP1 is a transcriptional cosuppressor for the Wilms' tumor suppressor protein WT1.",
abstract = "The Wilms' tumor suppressor protein WT1 is a transcriptional regulator that plays a key role in the development of the kidneys. The transcriptional activation domain of WT1 is subject to regulation by a suppression region within the N terminus of WT1. Using a functional assay, we provide direct evidence that this requires a transcriptional cosuppressor, which we identify as brain acid soluble protein 1 (BASP1). WT1 and BASP1 associate within the nuclei of cells that naturally express both proteins. BASP1 can confer WT1 cosuppressor activity in transfection assays, and elimination of endogenous BASP1 expression augments transcriptional activation by WT1. BASP1 is present in the developing nephron structures of the embryonic kidney and, coincident with that of WT1, its expression is restricted to the highly specialized podocyte cells of the adult kidney. Taken together, our results show that BASP1 is a WT1-associated factor that can regulate WT1 transcriptional activity. Udgivelsesdato: 2004-Jan",
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author = "Brian Carpenter and Hill, {Kathryn J} and Marika Charalambous and Wagner, {Kate J} and Diya Lahiri and James, {Dominic I} and Andersen, {Jens S} and Val{\'e}rie Schumacher and Brigitte Royer-Pokora and Matthias Mann and Andrew Ward and Roberts, {Stefan G E}",
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doi = "10.1128/MCB.24.2.537-549.2004",
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Carpenter, B, Hill, KJ, Charalambous, M, Wagner, KJ, Lahiri, D, James, DI, Andersen, JS, Schumacher, V, Royer-Pokora, B, Mann, M, Ward, A & Roberts, SGE 2004, 'BASP1 is a transcriptional cosuppressor for the Wilms' tumor suppressor protein WT1.', Molecular and Cellular Biology, bind 24, nr. 2, s. 537-549. https://doi.org/10.1128/MCB.24.2.537-549.2004

BASP1 is a transcriptional cosuppressor for the Wilms' tumor suppressor protein WT1. / Carpenter, Brian; Hill, Kathryn J; Charalambous, Marika; Wagner, Kate J; Lahiri, Diya; James, Dominic I; Andersen, Jens S; Schumacher, Valérie; Royer-Pokora, Brigitte; Mann, Matthias; Ward, Andrew; Roberts, Stefan G E.

I: Molecular and Cellular Biology, Bind 24, Nr. 2, 2004, s. 537-549.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - BASP1 is a transcriptional cosuppressor for the Wilms' tumor suppressor protein WT1.

AU - Carpenter, Brian

AU - Hill, Kathryn J

AU - Charalambous, Marika

AU - Wagner, Kate J

AU - Lahiri, Diya

AU - James, Dominic I

AU - Andersen, Jens S

AU - Schumacher, Valérie

AU - Royer-Pokora, Brigitte

AU - Mann, Matthias

AU - Ward, Andrew

AU - Roberts, Stefan G E

PY - 2004

Y1 - 2004

N2 - The Wilms' tumor suppressor protein WT1 is a transcriptional regulator that plays a key role in the development of the kidneys. The transcriptional activation domain of WT1 is subject to regulation by a suppression region within the N terminus of WT1. Using a functional assay, we provide direct evidence that this requires a transcriptional cosuppressor, which we identify as brain acid soluble protein 1 (BASP1). WT1 and BASP1 associate within the nuclei of cells that naturally express both proteins. BASP1 can confer WT1 cosuppressor activity in transfection assays, and elimination of endogenous BASP1 expression augments transcriptional activation by WT1. BASP1 is present in the developing nephron structures of the embryonic kidney and, coincident with that of WT1, its expression is restricted to the highly specialized podocyte cells of the adult kidney. Taken together, our results show that BASP1 is a WT1-associated factor that can regulate WT1 transcriptional activity. Udgivelsesdato: 2004-Jan

AB - The Wilms' tumor suppressor protein WT1 is a transcriptional regulator that plays a key role in the development of the kidneys. The transcriptional activation domain of WT1 is subject to regulation by a suppression region within the N terminus of WT1. Using a functional assay, we provide direct evidence that this requires a transcriptional cosuppressor, which we identify as brain acid soluble protein 1 (BASP1). WT1 and BASP1 associate within the nuclei of cells that naturally express both proteins. BASP1 can confer WT1 cosuppressor activity in transfection assays, and elimination of endogenous BASP1 expression augments transcriptional activation by WT1. BASP1 is present in the developing nephron structures of the embryonic kidney and, coincident with that of WT1, its expression is restricted to the highly specialized podocyte cells of the adult kidney. Taken together, our results show that BASP1 is a WT1-associated factor that can regulate WT1 transcriptional activity. Udgivelsesdato: 2004-Jan

KW - Adult

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - COS Cells

KW - Cell Line

KW - DNA, Complementary

KW - Female

KW - Gene Expression Regulation, Developmental

KW - Genes, Wilms Tumor

KW - Hela Cells

KW - Humans

KW - Kidney

KW - Male

KW - Membrane Proteins

KW - Mice

KW - Molecular Sequence Data

KW - Nerve Tissue Proteins

KW - Protein Structure, Tertiary

KW - Recombinant Fusion Proteins

KW - Repressor Proteins

KW - WT1 Proteins

U2 - 10.1128/MCB.24.2.537-549.2004

DO - 10.1128/MCB.24.2.537-549.2004

M3 - Journal article

VL - 24

SP - 537

EP - 549

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 2

ER -