Baseline growth and reproductive parameters in Lymnaea stagnalis for OECD test guideline development: optimization of diets and culturing conditions

J. Tasker, Henrik Holbech

Publikation: Konferencebidrag uden forlag/tidsskriftPosterForskning


The OECD has
successfully validated reproductive toxicity test guidelines with fish and
frogs for diverse chemicals (including endocrine disrupters). Since
molluscs are especially sensitive to chronic exposure to a number of
chemicals, the OECD supports the complementary development and
validation of a mollusc reproduction test guideline. An ad hoc mollusc
expert group has been formed in Europe to validate methods that can
meet this need. Currently, a key species for use in this context is the
freshwater gastropod Lymnaea stagnalis. An important aspect of this
work is to first develop a specific pathogen free defined strain of L.
stagnalis and second to establish a historical database of growth and
reproductive rates under defined culturing conditions. A mass culture of
the RENILYS® strain of L. stagnalis have been established at INRA
(France) since 2002 and has been distributed to research laboratories in
Denmark, Germany and the UK for the OECD pre-validation work to
date. Laboratory cultures of L. stagnalis are traditionally fed fresh
(preferably organic) lettuce; however, interrupted supplies of fresh
lettuce in some countries in 2011 highlighted a potential problem for the
draft OECD test guideline. Therefore, we have evaluated other diets
based on a review of the published literature and report here the results
for different feeds: namely, cabbage leaves, fish flakes, lettuce leaves or
sliced sweet potato. For the feeding trial, 5 snails (shell size ca. 2.6 cm)
were held in 1 L freshwater. Aeration was provided in all vessels, and
for all the vegetable diets treatments concentrations of ammonia, nitrate
and nitrite, the dissolved oxygen concentrations (>6 mg/L), pH (6.5-8.5)
and temperature (20±1ºC) ranges remained acceptable. However, the
fish flake diet gave low dissolved oxygen levels (< 10% saturation).
In this 28-d study, mean specific growth rates were highest for snails fed
lettuce ad libitum (0.09 mm/snail/day) followed by snails fed fish
flake>sweet potato>cabbage leave diets (0.03 mm/snail/day). Similarly,
in a 56-d ring-test study with 7 laboratories, the lettuce diet gave mean
specific growth rates of 0.06-0.09 mm/snail/day. Fecundity in the same
test ranged from 519-1424 total embryos per snail. These data will be
used towards developing a reference database of growth and
reproduction for L. stagnalis and to aid the statistical optimization of the
draft OECD test guideline for partial and full lifecycle testing of
Publikationsdato19. maj 2013
Antal sider1
StatusUdgivet - 19. maj 2013
BegivenhedSETAC Europe 23rd meeting - Glasgow, Storbritannien
Varighed: 12. maj 201316. maj 2013
Konferencens nummer: 23


KonferenceSETAC Europe 23rd meeting


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