Baltic Methanosarcina and Clostridium compete for electrons from metallic iron

Paola Andrea Palacios Jaramillo, Oona Snoeyenbos-West, Carolin Löscher, Bo Thamdrup, Amelia-Elena Rotaru

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Resumé

Microbial induced corrosion of steel structures, used for transport or storage of fuels, chemical weapons or waste radionuclides, is an environmental and economic threat. In non-sulfidic environments, the exact role of methanogens in steel corrosion is poorly understood. From the non-sulfidic, methanogenic sediments of the Baltic Sea corrosive communities were enriched using exclusively Fe0 as electron donor and CO2 as electron acceptor. Methane and acetate production were persistent for three years of successive transfers. Methanosarcina and Clostridium were attached to the Fe0, and dominated metagenome libraries. Since prior reports indicated Methanosarcina were merely commensals, consuming the acetate produced by acetogens, we investigated whether these methanogens were capable of Fe0 corrosion without bacterial partners (inhibited by an antibiotic cocktail). Unassisted, methanogens corroded Fe0 to Fe2+ at similar rates to the mixed community. Surprisingly, in the absence of competitive bacteria, Baltic-Methanosarcina produced six times more methane than they did in the mixed community. This signifies that Baltic-Methanosarcina achieved better corrosion alone, exclusive of an operative bacterial partner. Our results also show that together with acetogens, Methanosarcina interact competitively to retrieve electrons from Fe0 rather than as commensals as previously assumed.
OriginalsprogEngelsk
Artikelnummer530386
TidsskriftBioRxiv
DOI
StatusUdgivet - 25. jan. 2019

Fingeraftryk

corrosion
iron
electron
commensal
acetate
methane
chemical weapon
steel structure
antibiotics
radionuclide
steel
bacterium
economics
sediment

Citer dette

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title = "Baltic Methanosarcina and Clostridium compete for electrons from metallic iron",
abstract = "Microbial induced corrosion of steel structures, used for transport or storage of fuels, chemical weapons or waste radionuclides, is an environmental and economic threat. In non-sulfidic environments, the exact role of methanogens in steel corrosion is poorly understood. From the non-sulfidic, methanogenic sediments of the Baltic Sea corrosive communities were enriched using exclusively Fe0 as electron donor and CO2 as electron acceptor. Methane and acetate production were persistent for three years of successive transfers. Methanosarcina and Clostridium were attached to the Fe0, and dominated metagenome libraries. Since prior reports indicated Methanosarcina were merely commensals, consuming the acetate produced by acetogens, we investigated whether these methanogens were capable of Fe0 corrosion without bacterial partners (inhibited by an antibiotic cocktail). Unassisted, methanogens corroded Fe0 to Fe2+ at similar rates to the mixed community. Surprisingly, in the absence of competitive bacteria, Baltic-Methanosarcina produced six times more methane than they did in the mixed community. This signifies that Baltic-Methanosarcina achieved better corrosion alone, exclusive of an operative bacterial partner. Our results also show that together with acetogens, Methanosarcina interact competitively to retrieve electrons from Fe0 rather than as commensals as previously assumed.",
keywords = "Methanosarcina, Clostridium, iron corrosion, microbial induced corrosion, methanogens, acetogens, competitive interaction",
author = "{Palacios Jaramillo}, {Paola Andrea} and Oona Snoeyenbos-West and Carolin L{\"o}scher and Bo Thamdrup and Amelia-Elena Rotaru",
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Baltic Methanosarcina and Clostridium compete for electrons from metallic iron. / Palacios Jaramillo, Paola Andrea; Snoeyenbos-West, Oona; Löscher, Carolin; Thamdrup, Bo; Rotaru, Amelia-Elena.

I: BioRxiv, 25.01.2019.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskning

TY - JOUR

T1 - Baltic Methanosarcina and Clostridium compete for electrons from metallic iron

AU - Palacios Jaramillo, Paola Andrea

AU - Snoeyenbos-West, Oona

AU - Löscher, Carolin

AU - Thamdrup, Bo

AU - Rotaru, Amelia-Elena

PY - 2019/1/25

Y1 - 2019/1/25

N2 - Microbial induced corrosion of steel structures, used for transport or storage of fuels, chemical weapons or waste radionuclides, is an environmental and economic threat. In non-sulfidic environments, the exact role of methanogens in steel corrosion is poorly understood. From the non-sulfidic, methanogenic sediments of the Baltic Sea corrosive communities were enriched using exclusively Fe0 as electron donor and CO2 as electron acceptor. Methane and acetate production were persistent for three years of successive transfers. Methanosarcina and Clostridium were attached to the Fe0, and dominated metagenome libraries. Since prior reports indicated Methanosarcina were merely commensals, consuming the acetate produced by acetogens, we investigated whether these methanogens were capable of Fe0 corrosion without bacterial partners (inhibited by an antibiotic cocktail). Unassisted, methanogens corroded Fe0 to Fe2+ at similar rates to the mixed community. Surprisingly, in the absence of competitive bacteria, Baltic-Methanosarcina produced six times more methane than they did in the mixed community. This signifies that Baltic-Methanosarcina achieved better corrosion alone, exclusive of an operative bacterial partner. Our results also show that together with acetogens, Methanosarcina interact competitively to retrieve electrons from Fe0 rather than as commensals as previously assumed.

AB - Microbial induced corrosion of steel structures, used for transport or storage of fuels, chemical weapons or waste radionuclides, is an environmental and economic threat. In non-sulfidic environments, the exact role of methanogens in steel corrosion is poorly understood. From the non-sulfidic, methanogenic sediments of the Baltic Sea corrosive communities were enriched using exclusively Fe0 as electron donor and CO2 as electron acceptor. Methane and acetate production were persistent for three years of successive transfers. Methanosarcina and Clostridium were attached to the Fe0, and dominated metagenome libraries. Since prior reports indicated Methanosarcina were merely commensals, consuming the acetate produced by acetogens, we investigated whether these methanogens were capable of Fe0 corrosion without bacterial partners (inhibited by an antibiotic cocktail). Unassisted, methanogens corroded Fe0 to Fe2+ at similar rates to the mixed community. Surprisingly, in the absence of competitive bacteria, Baltic-Methanosarcina produced six times more methane than they did in the mixed community. This signifies that Baltic-Methanosarcina achieved better corrosion alone, exclusive of an operative bacterial partner. Our results also show that together with acetogens, Methanosarcina interact competitively to retrieve electrons from Fe0 rather than as commensals as previously assumed.

KW - Methanosarcina

KW - Clostridium

KW - iron corrosion

KW - microbial induced corrosion

KW - methanogens

KW - acetogens

KW - competitive interaction

U2 - 10.1101/530386

DO - 10.1101/530386

M3 - Journal article

JO - BioRxiv

JF - BioRxiv

M1 - 530386

ER -