Bacterial communities potentially involved in iron-cycling in Baltic Sea and North Sea sediments revealed by pyrosequencing

Carlen Reyes, Olaf Dellwig, K. Dähnke, Mathias Gehre, B. E. Noriega-Ortega, Michael E. Böttcher, P. Meister, Michael W. Friedrich

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

To gain insight into the bacterial communities involved in iron-(Fe) cycling under marine conditions, we analysed sediments with Fe-contents (0.5-1.5 wt %) from the suboxic zone at a marine site in the Skagerrak (SK) and a brackish site in the Bothnian Bay (BB) using 16S rRNA gene pyrosequencing. Several bacterial families, including Desulfobulbaceae, Desulfuromonadaceae and Pelobacteraceae and genera, including Desulfobacter and Geobacter, known to reduce Fe were detected and showed highest abundance near the Fe(III)/Fe(II) redox boundary. Additional genera with microorganisms capable of coupling fermentation to Fe-reduction, including Clostridium and Bacillus, were observed. Also, the Fe-oxidizing families Mariprofundaceae and Gallionellaceae occurred at the SK and BB sites, respectively, supporting Fe-cycling. In contrast, the sulphate (SO4 2-) reducing bacteria Desulfococcus and Desulfobacterium were more abundant at greater depths concurring with a decrease in Fe-reducing activity. The communities revealed by pyrosequencing, thus, match the redox stratification indicated by the geochemistry, with the known Fe-reducers coinciding with the zone of Fe-reduction. Not the intensely studied model organisms, such as Geobacter spp., but rather versatile microorganisms, including sulphate reducers and possibly unknown groups appear to be important for Fe-reduction in these marine suboxic sediments. FEMS 2016. All rights reserved.
OriginalsprogEngelsk
Artikelnummerfiw054
TidsskriftF E M S Microbiology Ecology
Vol/bind92
Udgave nummer4
Antal sider14
ISSN0168-6496
DOI
StatusUdgivet - 2016

Citer dette

Reyes, C., Dellwig, O., Dähnke, K., Gehre, M., Noriega-Ortega, B. E., Böttcher, M. E., ... Friedrich, M. W. (2016). Bacterial communities potentially involved in iron-cycling in Baltic Sea and North Sea sediments revealed by pyrosequencing. F E M S Microbiology Ecology, 92(4), [fiw054]. https://doi.org/10.1093/femsec/fiw054
Reyes, Carlen ; Dellwig, Olaf ; Dähnke, K. ; Gehre, Mathias ; Noriega-Ortega, B. E. ; Böttcher, Michael E. ; Meister, P. ; Friedrich, Michael W. / Bacterial communities potentially involved in iron-cycling in Baltic Sea and North Sea sediments revealed by pyrosequencing. I: F E M S Microbiology Ecology. 2016 ; Bind 92, Nr. 4.
@article{00580cf42f9a45438a0ed382fb8527f6,
title = "Bacterial communities potentially involved in iron-cycling in Baltic Sea and North Sea sediments revealed by pyrosequencing",
abstract = "To gain insight into the bacterial communities involved in iron-(Fe) cycling under marine conditions, we analysed sediments with Fe-contents (0.5-1.5 wt {\%}) from the suboxic zone at a marine site in the Skagerrak (SK) and a brackish site in the Bothnian Bay (BB) using 16S rRNA gene pyrosequencing. Several bacterial families, including Desulfobulbaceae, Desulfuromonadaceae and Pelobacteraceae and genera, including Desulfobacter and Geobacter, known to reduce Fe were detected and showed highest abundance near the Fe(III)/Fe(II) redox boundary. Additional genera with microorganisms capable of coupling fermentation to Fe-reduction, including Clostridium and Bacillus, were observed. Also, the Fe-oxidizing families Mariprofundaceae and Gallionellaceae occurred at the SK and BB sites, respectively, supporting Fe-cycling. In contrast, the sulphate (SO4 2-) reducing bacteria Desulfococcus and Desulfobacterium were more abundant at greater depths concurring with a decrease in Fe-reducing activity. The communities revealed by pyrosequencing, thus, match the redox stratification indicated by the geochemistry, with the known Fe-reducers coinciding with the zone of Fe-reduction. Not the intensely studied model organisms, such as Geobacter spp., but rather versatile microorganisms, including sulphate reducers and possibly unknown groups appear to be important for Fe-reduction in these marine suboxic sediments. FEMS 2016. All rights reserved.",
author = "Carlen Reyes and Olaf Dellwig and K. D{\"a}hnke and Mathias Gehre and Noriega-Ortega, {B. E.} and B{\"o}ttcher, {Michael E.} and P. Meister and Friedrich, {Michael W.}",
year = "2016",
doi = "10.1093/femsec/fiw054",
language = "English",
volume = "92",
journal = "F E M S Microbiology Ecology",
issn = "0168-6496",
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number = "4",

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Reyes, C, Dellwig, O, Dähnke, K, Gehre, M, Noriega-Ortega, BE, Böttcher, ME, Meister, P & Friedrich, MW 2016, 'Bacterial communities potentially involved in iron-cycling in Baltic Sea and North Sea sediments revealed by pyrosequencing', F E M S Microbiology Ecology, bind 92, nr. 4, fiw054. https://doi.org/10.1093/femsec/fiw054

Bacterial communities potentially involved in iron-cycling in Baltic Sea and North Sea sediments revealed by pyrosequencing. / Reyes, Carlen; Dellwig, Olaf; Dähnke, K.; Gehre, Mathias; Noriega-Ortega, B. E.; Böttcher, Michael E.; Meister, P.; Friedrich, Michael W.

I: F E M S Microbiology Ecology, Bind 92, Nr. 4, fiw054, 2016.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Bacterial communities potentially involved in iron-cycling in Baltic Sea and North Sea sediments revealed by pyrosequencing

AU - Reyes, Carlen

AU - Dellwig, Olaf

AU - Dähnke, K.

AU - Gehre, Mathias

AU - Noriega-Ortega, B. E.

AU - Böttcher, Michael E.

AU - Meister, P.

AU - Friedrich, Michael W.

PY - 2016

Y1 - 2016

N2 - To gain insight into the bacterial communities involved in iron-(Fe) cycling under marine conditions, we analysed sediments with Fe-contents (0.5-1.5 wt %) from the suboxic zone at a marine site in the Skagerrak (SK) and a brackish site in the Bothnian Bay (BB) using 16S rRNA gene pyrosequencing. Several bacterial families, including Desulfobulbaceae, Desulfuromonadaceae and Pelobacteraceae and genera, including Desulfobacter and Geobacter, known to reduce Fe were detected and showed highest abundance near the Fe(III)/Fe(II) redox boundary. Additional genera with microorganisms capable of coupling fermentation to Fe-reduction, including Clostridium and Bacillus, were observed. Also, the Fe-oxidizing families Mariprofundaceae and Gallionellaceae occurred at the SK and BB sites, respectively, supporting Fe-cycling. In contrast, the sulphate (SO4 2-) reducing bacteria Desulfococcus and Desulfobacterium were more abundant at greater depths concurring with a decrease in Fe-reducing activity. The communities revealed by pyrosequencing, thus, match the redox stratification indicated by the geochemistry, with the known Fe-reducers coinciding with the zone of Fe-reduction. Not the intensely studied model organisms, such as Geobacter spp., but rather versatile microorganisms, including sulphate reducers and possibly unknown groups appear to be important for Fe-reduction in these marine suboxic sediments. FEMS 2016. All rights reserved.

AB - To gain insight into the bacterial communities involved in iron-(Fe) cycling under marine conditions, we analysed sediments with Fe-contents (0.5-1.5 wt %) from the suboxic zone at a marine site in the Skagerrak (SK) and a brackish site in the Bothnian Bay (BB) using 16S rRNA gene pyrosequencing. Several bacterial families, including Desulfobulbaceae, Desulfuromonadaceae and Pelobacteraceae and genera, including Desulfobacter and Geobacter, known to reduce Fe were detected and showed highest abundance near the Fe(III)/Fe(II) redox boundary. Additional genera with microorganisms capable of coupling fermentation to Fe-reduction, including Clostridium and Bacillus, were observed. Also, the Fe-oxidizing families Mariprofundaceae and Gallionellaceae occurred at the SK and BB sites, respectively, supporting Fe-cycling. In contrast, the sulphate (SO4 2-) reducing bacteria Desulfococcus and Desulfobacterium were more abundant at greater depths concurring with a decrease in Fe-reducing activity. The communities revealed by pyrosequencing, thus, match the redox stratification indicated by the geochemistry, with the known Fe-reducers coinciding with the zone of Fe-reduction. Not the intensely studied model organisms, such as Geobacter spp., but rather versatile microorganisms, including sulphate reducers and possibly unknown groups appear to be important for Fe-reduction in these marine suboxic sediments. FEMS 2016. All rights reserved.

U2 - 10.1093/femsec/fiw054

DO - 10.1093/femsec/fiw054

M3 - Journal article

C2 - 26960392

VL - 92

JO - F E M S Microbiology Ecology

JF - F E M S Microbiology Ecology

SN - 0168-6496

IS - 4

M1 - fiw054

ER -