Bacillus Calmette-Guérin vaccination at birth and in vitro cytokine responses to non-specific stimulation. A randomized clinical trial

T N Nissen, N M Birk, B A Blok, R J W Arts, A Andersen, J Kjærgaard, L M Thøstesen, T Hoffmann, D L Jeppesen, S D Nielsen, P-E Kofoed, L G Stensballe, P Aaby, M Ruhwald, M G Netea, C S Benn, O Pryds

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Several studies have shown increased in vitro cytokine responses to non-related pathogens after Bacillus Calmette-Guérin (BCG) vaccination. A total of 158 infants (80 BCG administered within 7 days of birth; 78 controls) were bled 4 days post-randomization, and at age 3 and 13 months. Geometric mean concentrations of IL-1β, TNF-α, IL-6 (24 h stimulation) and IFN-γ, IL-10, IL-17, IL-22 (96 h stimulation) in response to in vitro stimulation with RPMI, LPS, PHA, Escherichia coli, Streptococcus pneumoniae, Candida albicans and BCG were compared among BCG vaccinated children and controls. BCG vaccination did not affect in vitro cytokine production, except IFN-γ and IL-22 response to BCG. Stratifying for 'age at randomization' we found a potentiating effect of BCG on cytokine production (TNF-α, IL-6, IL-10) in the 4 days post randomization stimulations, among children who were vaccinated at age 2-7 days versus age 0-1 days. BCG vaccination did not potentiate cytokine production to non-BCG antigens. At 4 days post randomization, BCG was associated with higher cytokine production in the later randomized children.

OriginalsprogEngelsk
TidsskriftEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
Vol/bind37
Udgave nummer1
Sider (fra-til)29–41
ISSN0934-9723
DOI
StatusUdgivet - jan. 2018

Fingeraftryk

Randomized Controlled Trials
Random Allocation
Interleukin-10
Interleukin-6
In Vitro Techniques
Interleukin-17

Citer dette

Nissen, T N ; Birk, N M ; Blok, B A ; Arts, R J W ; Andersen, A ; Kjærgaard, J ; Thøstesen, L M ; Hoffmann, T ; Jeppesen, D L ; Nielsen, S D ; Kofoed, P-E ; Stensballe, L G ; Aaby, P ; Ruhwald, M ; Netea, M G ; Benn, C S ; Pryds, O. / Bacillus Calmette-Guérin vaccination at birth and in vitro cytokine responses to non-specific stimulation. A randomized clinical trial. I: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology. 2018 ; Bind 37, Nr. 1. s. 29–41.
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abstract = "Several studies have shown increased in vitro cytokine responses to non-related pathogens after Bacillus Calmette-Gu{\'e}rin (BCG) vaccination. A total of 158 infants (80 BCG administered within 7 days of birth; 78 controls) were bled 4 days post-randomization, and at age 3 and 13 months. Geometric mean concentrations of IL-1β, TNF-α, IL-6 (24 h stimulation) and IFN-γ, IL-10, IL-17, IL-22 (96 h stimulation) in response to in vitro stimulation with RPMI, LPS, PHA, Escherichia coli, Streptococcus pneumoniae, Candida albicans and BCG were compared among BCG vaccinated children and controls. BCG vaccination did not affect in vitro cytokine production, except IFN-γ and IL-22 response to BCG. Stratifying for 'age at randomization' we found a potentiating effect of BCG on cytokine production (TNF-α, IL-6, IL-10) in the 4 days post randomization stimulations, among children who were vaccinated at age 2-7 days versus age 0-1 days. BCG vaccination did not potentiate cytokine production to non-BCG antigens. At 4 days post randomization, BCG was associated with higher cytokine production in the later randomized children.",
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Bacillus Calmette-Guérin vaccination at birth and in vitro cytokine responses to non-specific stimulation. A randomized clinical trial. / Nissen, T N; Birk, N M; Blok, B A; Arts, R J W; Andersen, A; Kjærgaard, J; Thøstesen, L M; Hoffmann, T; Jeppesen, D L; Nielsen, S D; Kofoed, P-E; Stensballe, L G; Aaby, P; Ruhwald, M; Netea, M G; Benn, C S; Pryds, O.

I: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, Bind 37, Nr. 1, 01.2018, s. 29–41.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Bacillus Calmette-Guérin vaccination at birth and in vitro cytokine responses to non-specific stimulation. A randomized clinical trial

AU - Nissen, T N

AU - Birk, N M

AU - Blok, B A

AU - Arts, R J W

AU - Andersen, A

AU - Kjærgaard, J

AU - Thøstesen, L M

AU - Hoffmann, T

AU - Jeppesen, D L

AU - Nielsen, S D

AU - Kofoed, P-E

AU - Stensballe, L G

AU - Aaby, P

AU - Ruhwald, M

AU - Netea, M G

AU - Benn, C S

AU - Pryds, O

PY - 2018/1

Y1 - 2018/1

N2 - Several studies have shown increased in vitro cytokine responses to non-related pathogens after Bacillus Calmette-Guérin (BCG) vaccination. A total of 158 infants (80 BCG administered within 7 days of birth; 78 controls) were bled 4 days post-randomization, and at age 3 and 13 months. Geometric mean concentrations of IL-1β, TNF-α, IL-6 (24 h stimulation) and IFN-γ, IL-10, IL-17, IL-22 (96 h stimulation) in response to in vitro stimulation with RPMI, LPS, PHA, Escherichia coli, Streptococcus pneumoniae, Candida albicans and BCG were compared among BCG vaccinated children and controls. BCG vaccination did not affect in vitro cytokine production, except IFN-γ and IL-22 response to BCG. Stratifying for 'age at randomization' we found a potentiating effect of BCG on cytokine production (TNF-α, IL-6, IL-10) in the 4 days post randomization stimulations, among children who were vaccinated at age 2-7 days versus age 0-1 days. BCG vaccination did not potentiate cytokine production to non-BCG antigens. At 4 days post randomization, BCG was associated with higher cytokine production in the later randomized children.

AB - Several studies have shown increased in vitro cytokine responses to non-related pathogens after Bacillus Calmette-Guérin (BCG) vaccination. A total of 158 infants (80 BCG administered within 7 days of birth; 78 controls) were bled 4 days post-randomization, and at age 3 and 13 months. Geometric mean concentrations of IL-1β, TNF-α, IL-6 (24 h stimulation) and IFN-γ, IL-10, IL-17, IL-22 (96 h stimulation) in response to in vitro stimulation with RPMI, LPS, PHA, Escherichia coli, Streptococcus pneumoniae, Candida albicans and BCG were compared among BCG vaccinated children and controls. BCG vaccination did not affect in vitro cytokine production, except IFN-γ and IL-22 response to BCG. Stratifying for 'age at randomization' we found a potentiating effect of BCG on cytokine production (TNF-α, IL-6, IL-10) in the 4 days post randomization stimulations, among children who were vaccinated at age 2-7 days versus age 0-1 days. BCG vaccination did not potentiate cytokine production to non-BCG antigens. At 4 days post randomization, BCG was associated with higher cytokine production in the later randomized children.

KW - Journal Article

KW - Bacillus Calmette-Guérin

KW - Randomized clinical trial

KW - Cytokine response

KW - Heterolougus immunity

KW - Infants

KW - Trained immunity

KW - Vaccines

KW - Mycobacterium bovis/immunology

KW - Humans

KW - Vaccination

KW - Male

KW - Cytokines/blood

KW - Escherichia coli/immunology

KW - Streptococcus pneumoniae/immunology

KW - Candida albicans/immunology

KW - BCG Vaccine/administration & dosage

KW - Female

KW - Infant, Newborn

U2 - 10.1007/s10096-017-3097-2

DO - 10.1007/s10096-017-3097-2

M3 - Journal article

C2 - 28890996

VL - 37

SP - 29

EP - 41

JO - European Journal of Clinical Microbiology & Infectious Diseases

JF - European Journal of Clinical Microbiology & Infectious Diseases

SN - 0934-9723

IS - 1

ER -