Autoantibody profiling in lupus patients using synthetic nucleic acids

Martin Klecka, Christina Thybo, Claudia Macaubas, Ilia Solov'yov, Julia Simard, Imelda Maria Balboni, Emily Fox, Anne Voss, Elizabeth D. Mellins, Kira Astakhova*

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Resumé

Autoantibodies to nuclear components of cells (antinuclear antibodies, ANA), including DNA (a-DNA), are widely used in the diagnosis and subtyping of certain autoimmune diseases, including systemic lupus erythematosus (SLE). Despite clinical use over decades, precise, reproducible measurement of a-DNA titers remains difficult, likely due to the substantial sequence and length heterogeneity of DNA purified from natural sources. We designed and tested a panel of synthetic nucleic acid molecules composed of native deoxyribonucleotide units to measure a-DNA. ELISA assays using these antigens show specificity and reproducibility. Applying the ELISA tests to serological studies of pediatric and adult SLE, we identified novel clinical correlations. We also observed preferential recognition of a specific synthetic antigen by antibodies in SLE sera. We determined the probable basis for this finding using computational analyses, providing valuable structural information for future development of DNA antigens. Synthetic nucleic acid molecules offer the opportunity to standardize assays and to dissect antibody-Antigen interactions.

OriginalsprogEngelsk
Artikelnummer5554
TidsskriftScientific Reports
Vol/bind8
Udgave nummer1
Antal sider9
ISSN2045-2322
DOI
StatusUdgivet - 3. apr. 2018

Fingeraftryk

Nucleic Acids
DNA
Cellular Structures
Pediatrics
Serum

Citer dette

Klecka, M., Thybo, C., Macaubas, C., Solov'yov, I., Simard, J., Balboni, I. M., ... Astakhova, K. (2018). Autoantibody profiling in lupus patients using synthetic nucleic acids. Scientific Reports, 8(1), [5554]. https://doi.org/10.1038/s41598-018-23910-5
Klecka, Martin ; Thybo, Christina ; Macaubas, Claudia ; Solov'yov, Ilia ; Simard, Julia ; Balboni, Imelda Maria ; Fox, Emily ; Voss, Anne ; Mellins, Elizabeth D. ; Astakhova, Kira. / Autoantibody profiling in lupus patients using synthetic nucleic acids. I: Scientific Reports. 2018 ; Bind 8, Nr. 1.
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title = "Autoantibody profiling in lupus patients using synthetic nucleic acids",
abstract = "Autoantibodies to nuclear components of cells (antinuclear antibodies, ANA), including DNA (a-DNA), are widely used in the diagnosis and subtyping of certain autoimmune diseases, including systemic lupus erythematosus (SLE). Despite clinical use over decades, precise, reproducible measurement of a-DNA titers remains difficult, likely due to the substantial sequence and length heterogeneity of DNA purified from natural sources. We designed and tested a panel of synthetic nucleic acid molecules composed of native deoxyribonucleotide units to measure a-DNA. ELISA assays using these antigens show specificity and reproducibility. Applying the ELISA tests to serological studies of pediatric and adult SLE, we identified novel clinical correlations. We also observed preferential recognition of a specific synthetic antigen by antibodies in SLE sera. We determined the probable basis for this finding using computational analyses, providing valuable structural information for future development of DNA antigens. Synthetic nucleic acid molecules offer the opportunity to standardize assays and to dissect antibody-Antigen interactions.",
author = "Martin Klecka and Christina Thybo and Claudia Macaubas and Ilia Solov'yov and Julia Simard and Balboni, {Imelda Maria} and Emily Fox and Anne Voss and Mellins, {Elizabeth D.} and Kira Astakhova",
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Klecka, M, Thybo, C, Macaubas, C, Solov'yov, I, Simard, J, Balboni, IM, Fox, E, Voss, A, Mellins, ED & Astakhova, K 2018, 'Autoantibody profiling in lupus patients using synthetic nucleic acids', Scientific Reports, bind 8, nr. 1, 5554. https://doi.org/10.1038/s41598-018-23910-5

Autoantibody profiling in lupus patients using synthetic nucleic acids. / Klecka, Martin; Thybo, Christina; Macaubas, Claudia; Solov'yov, Ilia; Simard, Julia; Balboni, Imelda Maria; Fox, Emily; Voss, Anne; Mellins, Elizabeth D.; Astakhova, Kira.

I: Scientific Reports, Bind 8, Nr. 1, 5554, 03.04.2018.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Autoantibody profiling in lupus patients using synthetic nucleic acids

AU - Klecka, Martin

AU - Thybo, Christina

AU - Macaubas, Claudia

AU - Solov'yov, Ilia

AU - Simard, Julia

AU - Balboni, Imelda Maria

AU - Fox, Emily

AU - Voss, Anne

AU - Mellins, Elizabeth D.

AU - Astakhova, Kira

PY - 2018/4/3

Y1 - 2018/4/3

N2 - Autoantibodies to nuclear components of cells (antinuclear antibodies, ANA), including DNA (a-DNA), are widely used in the diagnosis and subtyping of certain autoimmune diseases, including systemic lupus erythematosus (SLE). Despite clinical use over decades, precise, reproducible measurement of a-DNA titers remains difficult, likely due to the substantial sequence and length heterogeneity of DNA purified from natural sources. We designed and tested a panel of synthetic nucleic acid molecules composed of native deoxyribonucleotide units to measure a-DNA. ELISA assays using these antigens show specificity and reproducibility. Applying the ELISA tests to serological studies of pediatric and adult SLE, we identified novel clinical correlations. We also observed preferential recognition of a specific synthetic antigen by antibodies in SLE sera. We determined the probable basis for this finding using computational analyses, providing valuable structural information for future development of DNA antigens. Synthetic nucleic acid molecules offer the opportunity to standardize assays and to dissect antibody-Antigen interactions.

AB - Autoantibodies to nuclear components of cells (antinuclear antibodies, ANA), including DNA (a-DNA), are widely used in the diagnosis and subtyping of certain autoimmune diseases, including systemic lupus erythematosus (SLE). Despite clinical use over decades, precise, reproducible measurement of a-DNA titers remains difficult, likely due to the substantial sequence and length heterogeneity of DNA purified from natural sources. We designed and tested a panel of synthetic nucleic acid molecules composed of native deoxyribonucleotide units to measure a-DNA. ELISA assays using these antigens show specificity and reproducibility. Applying the ELISA tests to serological studies of pediatric and adult SLE, we identified novel clinical correlations. We also observed preferential recognition of a specific synthetic antigen by antibodies in SLE sera. We determined the probable basis for this finding using computational analyses, providing valuable structural information for future development of DNA antigens. Synthetic nucleic acid molecules offer the opportunity to standardize assays and to dissect antibody-Antigen interactions.

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