ATP synthesis is impaired in isolated mitochondria from myotubes established from type 2 diabetic subjects

Ariane D Minet, Michael Gaster

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

To date, it is unknown whether mitochondrial dysfunction in skeletal muscle from subjects with type 2 diabetes is based on primarily reduced mitochondrial mass and/or a primarily decreased mitochondrial ATP synthesis. Mitochondrial mass were determined in myotubes established from eight lean, eight obese and eight subjects with type 2 diabetes precultured under normophysiological conditions. Furthermore, mitochondria were isolated and ATP production was measured by luminescence at baseline and during acute insulin stimulation with or without concomitant ATP utilization by hexokinase. Mitochondrial mass and the ATP synthesis rate, neither at baseline nor during acute insulin stimulation, were not different between groups. The ratio of ATP synthesis rate at hexokinase versus ATP synthesis rate at baseline was lower in diabetic mitochondria compared to lean mitochondria. Thus the lower content of muscle mitochondria in type 2 diabetes in vivo is an adaptive trait and mitochondrial dysfunction in type 2 diabetes in vivo is based both on primarily impaired ATP synthesis and an adaptive loss of mitochondrial mass.
OriginalsprogEngelsk
TidsskriftBiochemical and Biophysical Research Communications
Vol/bind402
Udgave nummer1
Sider (fra-til)70-4
Antal sider5
ISSN0006-291X
DOI
StatusUdgivet - 5. nov. 2010

Fingeraftryk

Mitochondria
Adenosine Triphosphate
Medical problems
Type 2 Diabetes Mellitus
Hexokinase
Muscle
Insulin
Luminescence
Skeletal Muscle

Citer dette

@article{cad291d7c33444858f15e6139a89d5b0,
title = "ATP synthesis is impaired in isolated mitochondria from myotubes established from type 2 diabetic subjects",
abstract = "To date, it is unknown whether mitochondrial dysfunction in skeletal muscle from subjects with type 2 diabetes is based on primarily reduced mitochondrial mass and/or a primarily decreased mitochondrial ATP synthesis. Mitochondrial mass were determined in myotubes established from eight lean, eight obese and eight subjects with type 2 diabetes precultured under normophysiological conditions. Furthermore, mitochondria were isolated and ATP production was measured by luminescence at baseline and during acute insulin stimulation with or without concomitant ATP utilization by hexokinase. Mitochondrial mass and the ATP synthesis rate, neither at baseline nor during acute insulin stimulation, were not different between groups. The ratio of ATP synthesis rate at hexokinase versus ATP synthesis rate at baseline was lower in diabetic mitochondria compared to lean mitochondria. Thus the lower content of muscle mitochondria in type 2 diabetes in vivo is an adaptive trait and mitochondrial dysfunction in type 2 diabetes in vivo is based both on primarily impaired ATP synthesis and an adaptive loss of mitochondrial mass.",
keywords = "Adenosine Triphosphate, Cells, Cultured, Diabetes Mellitus, Type 2, Humans, Middle Aged, Muscle Fibers, Skeletal",
author = "Minet, {Ariane D} and Michael Gaster",
note = "Copyright {\circledC} 2010 Elsevier Inc. All rights reserved.",
year = "2010",
month = "11",
day = "5",
doi = "10.1016/j.bbrc.2010.09.115",
language = "English",
volume = "402",
pages = "70--4",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",
number = "1",

}

ATP synthesis is impaired in isolated mitochondria from myotubes established from type 2 diabetic subjects. / Minet, Ariane D; Gaster, Michael.

I: Biochemical and Biophysical Research Communications, Bind 402, Nr. 1, 05.11.2010, s. 70-4.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - ATP synthesis is impaired in isolated mitochondria from myotubes established from type 2 diabetic subjects

AU - Minet, Ariane D

AU - Gaster, Michael

N1 - Copyright © 2010 Elsevier Inc. All rights reserved.

PY - 2010/11/5

Y1 - 2010/11/5

N2 - To date, it is unknown whether mitochondrial dysfunction in skeletal muscle from subjects with type 2 diabetes is based on primarily reduced mitochondrial mass and/or a primarily decreased mitochondrial ATP synthesis. Mitochondrial mass were determined in myotubes established from eight lean, eight obese and eight subjects with type 2 diabetes precultured under normophysiological conditions. Furthermore, mitochondria were isolated and ATP production was measured by luminescence at baseline and during acute insulin stimulation with or without concomitant ATP utilization by hexokinase. Mitochondrial mass and the ATP synthesis rate, neither at baseline nor during acute insulin stimulation, were not different between groups. The ratio of ATP synthesis rate at hexokinase versus ATP synthesis rate at baseline was lower in diabetic mitochondria compared to lean mitochondria. Thus the lower content of muscle mitochondria in type 2 diabetes in vivo is an adaptive trait and mitochondrial dysfunction in type 2 diabetes in vivo is based both on primarily impaired ATP synthesis and an adaptive loss of mitochondrial mass.

AB - To date, it is unknown whether mitochondrial dysfunction in skeletal muscle from subjects with type 2 diabetes is based on primarily reduced mitochondrial mass and/or a primarily decreased mitochondrial ATP synthesis. Mitochondrial mass were determined in myotubes established from eight lean, eight obese and eight subjects with type 2 diabetes precultured under normophysiological conditions. Furthermore, mitochondria were isolated and ATP production was measured by luminescence at baseline and during acute insulin stimulation with or without concomitant ATP utilization by hexokinase. Mitochondrial mass and the ATP synthesis rate, neither at baseline nor during acute insulin stimulation, were not different between groups. The ratio of ATP synthesis rate at hexokinase versus ATP synthesis rate at baseline was lower in diabetic mitochondria compared to lean mitochondria. Thus the lower content of muscle mitochondria in type 2 diabetes in vivo is an adaptive trait and mitochondrial dysfunction in type 2 diabetes in vivo is based both on primarily impaired ATP synthesis and an adaptive loss of mitochondrial mass.

KW - Adenosine Triphosphate

KW - Cells, Cultured

KW - Diabetes Mellitus, Type 2

KW - Humans

KW - Middle Aged

KW - Muscle Fibers, Skeletal

U2 - 10.1016/j.bbrc.2010.09.115

DO - 10.1016/j.bbrc.2010.09.115

M3 - Journal article

VL - 402

SP - 70

EP - 74

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -