Association of polymorphism of ser311cys paraoxonase-2 gene with type 2 diabetes mellitus in iran

Sasan Andalib, Golnaz Vaseghi, Azadeh Motavallian, Hamid Mirmohamad Sadeghi, Azadeh Eshraghi, Masoud Amini, Amir-Reza Majlesi

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

BACKGROUND: It is believed that paraoxonase-2 gene polymorphism is associated with type 2 diabetes. This study is aimed to investigate the association between paraoxonase-2 gene polymorphism and type 2 diabetes in an Iranian population.

METHODS: This study was performed on 200 individuals including 100 diabetics and 100 healthy subjects. Genomic DNA was extracted from peripheral blood leukocytes, and PCR-RFLP was carried out. Palindromic sequence in PON2 gene was recognized by Dde1 restriction endonuclease. In order to visualize restriction products, electrophoresis was carried out using polyacrylamide gel (8%) and ethidium bromide staining.

RESULTS: The expected PCR product of 331 bp was obtained. Digestion of this product with DdeI showed four Ser homozygotes, three Cys homozygotes, and five Ser311 Cys heterozygotes. The gene frequency of Cys (C) in diabetic subjects was significantly higher than in healthy subjects.

CONCLUSIONS: This study suggests that an association exists between Ser311 Cys polymorphism and type 2 diabetes mellitus.

OriginalsprogEngelsk
TidsskriftInternational Journal of Preventive Medicine
Vol/bind4
Udgave nummer5
Sider (fra-til)517-22
Antal sider6
ISSN2008-7802
StatusUdgivet - maj 2013

Fingeraftryk

Aryldialkylphosphatase
Iran
Type 2 Diabetes Mellitus
Homozygote
Polymerase Chain Reaction
Ethidium
DNA Restriction Enzymes
Heterozygote
Restriction Fragment Length Polymorphisms
DNA
Population

Citer dette

Andalib, S., Vaseghi, G., Motavallian, A., Sadeghi, H. M., Eshraghi, A., Amini, M., & Majlesi, A-R. (2013). Association of polymorphism of ser311cys paraoxonase-2 gene with type 2 diabetes mellitus in iran. International Journal of Preventive Medicine, 4(5), 517-22.
Andalib, Sasan ; Vaseghi, Golnaz ; Motavallian, Azadeh ; Sadeghi, Hamid Mirmohamad ; Eshraghi, Azadeh ; Amini, Masoud ; Majlesi, Amir-Reza. / Association of polymorphism of ser311cys paraoxonase-2 gene with type 2 diabetes mellitus in iran. I: International Journal of Preventive Medicine. 2013 ; Bind 4, Nr. 5. s. 517-22.
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title = "Association of polymorphism of ser311cys paraoxonase-2 gene with type 2 diabetes mellitus in iran",
abstract = "BACKGROUND: It is believed that paraoxonase-2 gene polymorphism is associated with type 2 diabetes. This study is aimed to investigate the association between paraoxonase-2 gene polymorphism and type 2 diabetes in an Iranian population.METHODS: This study was performed on 200 individuals including 100 diabetics and 100 healthy subjects. Genomic DNA was extracted from peripheral blood leukocytes, and PCR-RFLP was carried out. Palindromic sequence in PON2 gene was recognized by Dde1 restriction endonuclease. In order to visualize restriction products, electrophoresis was carried out using polyacrylamide gel (8{\%}) and ethidium bromide staining.RESULTS: The expected PCR product of 331 bp was obtained. Digestion of this product with DdeI showed four Ser homozygotes, three Cys homozygotes, and five Ser311 Cys heterozygotes. The gene frequency of Cys (C) in diabetic subjects was significantly higher than in healthy subjects.CONCLUSIONS: This study suggests that an association exists between Ser311 Cys polymorphism and type 2 diabetes mellitus.",
author = "Sasan Andalib and Golnaz Vaseghi and Azadeh Motavallian and Sadeghi, {Hamid Mirmohamad} and Azadeh Eshraghi and Masoud Amini and Amir-Reza Majlesi",
year = "2013",
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pages = "517--22",
journal = "International Journal of Preventive Medicine",
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Andalib, S, Vaseghi, G, Motavallian, A, Sadeghi, HM, Eshraghi, A, Amini, M & Majlesi, A-R 2013, 'Association of polymorphism of ser311cys paraoxonase-2 gene with type 2 diabetes mellitus in iran', International Journal of Preventive Medicine, bind 4, nr. 5, s. 517-22.

Association of polymorphism of ser311cys paraoxonase-2 gene with type 2 diabetes mellitus in iran. / Andalib, Sasan; Vaseghi, Golnaz; Motavallian, Azadeh; Sadeghi, Hamid Mirmohamad; Eshraghi, Azadeh; Amini, Masoud; Majlesi, Amir-Reza.

I: International Journal of Preventive Medicine, Bind 4, Nr. 5, 05.2013, s. 517-22.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Association of polymorphism of ser311cys paraoxonase-2 gene with type 2 diabetes mellitus in iran

AU - Andalib, Sasan

AU - Vaseghi, Golnaz

AU - Motavallian, Azadeh

AU - Sadeghi, Hamid Mirmohamad

AU - Eshraghi, Azadeh

AU - Amini, Masoud

AU - Majlesi, Amir-Reza

PY - 2013/5

Y1 - 2013/5

N2 - BACKGROUND: It is believed that paraoxonase-2 gene polymorphism is associated with type 2 diabetes. This study is aimed to investigate the association between paraoxonase-2 gene polymorphism and type 2 diabetes in an Iranian population.METHODS: This study was performed on 200 individuals including 100 diabetics and 100 healthy subjects. Genomic DNA was extracted from peripheral blood leukocytes, and PCR-RFLP was carried out. Palindromic sequence in PON2 gene was recognized by Dde1 restriction endonuclease. In order to visualize restriction products, electrophoresis was carried out using polyacrylamide gel (8%) and ethidium bromide staining.RESULTS: The expected PCR product of 331 bp was obtained. Digestion of this product with DdeI showed four Ser homozygotes, three Cys homozygotes, and five Ser311 Cys heterozygotes. The gene frequency of Cys (C) in diabetic subjects was significantly higher than in healthy subjects.CONCLUSIONS: This study suggests that an association exists between Ser311 Cys polymorphism and type 2 diabetes mellitus.

AB - BACKGROUND: It is believed that paraoxonase-2 gene polymorphism is associated with type 2 diabetes. This study is aimed to investigate the association between paraoxonase-2 gene polymorphism and type 2 diabetes in an Iranian population.METHODS: This study was performed on 200 individuals including 100 diabetics and 100 healthy subjects. Genomic DNA was extracted from peripheral blood leukocytes, and PCR-RFLP was carried out. Palindromic sequence in PON2 gene was recognized by Dde1 restriction endonuclease. In order to visualize restriction products, electrophoresis was carried out using polyacrylamide gel (8%) and ethidium bromide staining.RESULTS: The expected PCR product of 331 bp was obtained. Digestion of this product with DdeI showed four Ser homozygotes, three Cys homozygotes, and five Ser311 Cys heterozygotes. The gene frequency of Cys (C) in diabetic subjects was significantly higher than in healthy subjects.CONCLUSIONS: This study suggests that an association exists between Ser311 Cys polymorphism and type 2 diabetes mellitus.

M3 - Journal article

C2 - 23930161

VL - 4

SP - 517

EP - 522

JO - International Journal of Preventive Medicine

JF - International Journal of Preventive Medicine

SN - 2008-7802

IS - 5

ER -