Association between age and repair of oxidatively damaged DNA in human peripheral blood mononuclear cells

Mille Løhr, Annie Jensen, Louise Eriksen, Morten Grønbæk, Steffen Loft, Peter Møller

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

It has been hypothesised that positive associations between age and levels of oxidative stress-generated damage to DNA may be related to an age-dependent decline in DNA repair activity. The objective of this study was to investigate the association between age and repair activity of oxidatively damaged DNA in peripheral blood mononuclear cells (PBMCs). We isolated PBMCs from subjects aged 18-83 years, as part of a health survey of the Danish population that focussed on lifestyle factors. The level of DNA repair activity was measured as incisions on potassium bromate-damaged DNA by the comet assay. There was an inverse association between age and DNA repair activity with a 0.65% decline in activity per year from age 18 to 83 (95% confidence interval: 0.16-1.14% per year). Univariate regression analysis also indicated inverse associations between DNA repair activity and waist-hip ratio (P < 0.05) and plasma concentrations of glycosylated hemoglobin (P = 0.07). However, multivariate regression analysis only showed an inverse association between age and DNA repair activity (P < 0.05), indicating that the decline in repair activity was not mediated by metabolic risk factors. In summary, the results show an inverse association between age and DNA repair activity of oxidatively damaged DNA.

OriginalsprogEngelsk
TidsskriftMutagenesis
Vol/bind30
Udgave nummer5
Sider (fra-til)695-700
Antal sider6
ISSN0267-8357
DOI
StatusUdgivet - sep. 2015

Fingeraftryk

Repair
Blood
DNA
Association reactions
Regression Analysis
Comet Assay
Regression analysis
Glycosylated Hemoglobin A
Health Surveys
Multivariate Analysis
Oxidative stress
Confidence Intervals
Assays
Population
Plasmas

Citer dette

Løhr, Mille ; Jensen, Annie ; Eriksen, Louise ; Grønbæk, Morten ; Loft, Steffen ; Møller, Peter. / Association between age and repair of oxidatively damaged DNA in human peripheral blood mononuclear cells. I: Mutagenesis. 2015 ; Bind 30, Nr. 5. s. 695-700.
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abstract = "It has been hypothesised that positive associations between age and levels of oxidative stress-generated damage to DNA may be related to an age-dependent decline in DNA repair activity. The objective of this study was to investigate the association between age and repair activity of oxidatively damaged DNA in peripheral blood mononuclear cells (PBMCs). We isolated PBMCs from subjects aged 18-83 years, as part of a health survey of the Danish population that focussed on lifestyle factors. The level of DNA repair activity was measured as incisions on potassium bromate-damaged DNA by the comet assay. There was an inverse association between age and DNA repair activity with a 0.65{\%} decline in activity per year from age 18 to 83 (95{\%} confidence interval: 0.16-1.14{\%} per year). Univariate regression analysis also indicated inverse associations between DNA repair activity and waist-hip ratio (P < 0.05) and plasma concentrations of glycosylated hemoglobin (P = 0.07). However, multivariate regression analysis only showed an inverse association between age and DNA repair activity (P < 0.05), indicating that the decline in repair activity was not mediated by metabolic risk factors. In summary, the results show an inverse association between age and DNA repair activity of oxidatively damaged DNA.",
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Association between age and repair of oxidatively damaged DNA in human peripheral blood mononuclear cells. / Løhr, Mille; Jensen, Annie; Eriksen, Louise; Grønbæk, Morten; Loft, Steffen; Møller, Peter.

I: Mutagenesis, Bind 30, Nr. 5, 09.2015, s. 695-700.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Association between age and repair of oxidatively damaged DNA in human peripheral blood mononuclear cells

AU - Løhr, Mille

AU - Jensen, Annie

AU - Eriksen, Louise

AU - Grønbæk, Morten

AU - Loft, Steffen

AU - Møller, Peter

N1 - © The Author 2015. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

PY - 2015/9

Y1 - 2015/9

N2 - It has been hypothesised that positive associations between age and levels of oxidative stress-generated damage to DNA may be related to an age-dependent decline in DNA repair activity. The objective of this study was to investigate the association between age and repair activity of oxidatively damaged DNA in peripheral blood mononuclear cells (PBMCs). We isolated PBMCs from subjects aged 18-83 years, as part of a health survey of the Danish population that focussed on lifestyle factors. The level of DNA repair activity was measured as incisions on potassium bromate-damaged DNA by the comet assay. There was an inverse association between age and DNA repair activity with a 0.65% decline in activity per year from age 18 to 83 (95% confidence interval: 0.16-1.14% per year). Univariate regression analysis also indicated inverse associations between DNA repair activity and waist-hip ratio (P < 0.05) and plasma concentrations of glycosylated hemoglobin (P = 0.07). However, multivariate regression analysis only showed an inverse association between age and DNA repair activity (P < 0.05), indicating that the decline in repair activity was not mediated by metabolic risk factors. In summary, the results show an inverse association between age and DNA repair activity of oxidatively damaged DNA.

AB - It has been hypothesised that positive associations between age and levels of oxidative stress-generated damage to DNA may be related to an age-dependent decline in DNA repair activity. The objective of this study was to investigate the association between age and repair activity of oxidatively damaged DNA in peripheral blood mononuclear cells (PBMCs). We isolated PBMCs from subjects aged 18-83 years, as part of a health survey of the Danish population that focussed on lifestyle factors. The level of DNA repair activity was measured as incisions on potassium bromate-damaged DNA by the comet assay. There was an inverse association between age and DNA repair activity with a 0.65% decline in activity per year from age 18 to 83 (95% confidence interval: 0.16-1.14% per year). Univariate regression analysis also indicated inverse associations between DNA repair activity and waist-hip ratio (P < 0.05) and plasma concentrations of glycosylated hemoglobin (P = 0.07). However, multivariate regression analysis only showed an inverse association between age and DNA repair activity (P < 0.05), indicating that the decline in repair activity was not mediated by metabolic risk factors. In summary, the results show an inverse association between age and DNA repair activity of oxidatively damaged DNA.

U2 - 10.1093/mutage/gev031

DO - 10.1093/mutage/gev031

M3 - Journal article

C2 - 25925070

VL - 30

SP - 695

EP - 700

JO - Mutagenesis

JF - Mutagenesis

SN - 0267-8357

IS - 5

ER -