Assessment of the NMR visibility of intraerythrocytic sodium by flame photometric and ion‐competitive studies

Henrik Nissen*, Jens Peter Jacobsen, Mogens Hørder

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

23Na nuclear magnetic resonance (NMR) studies of intraerythrocytic sodium concentrations were performed at 37°C and the accuracy of the method was evaluated through concomitant emission flame photometric analysis. NMR visibility of intraerythrocytic sodium was estimated to be 75 ± 3%. Verification of the presence of an NMR invisible intraerythrocytic sodium pool and assessment of the mechanisms explaining this invisibility was achieved by measurement of 23Na NMR signal intensities and T1 relaxation times of erythrocyte hemolysate during ion‐competitive sodium release from association sites. Signal intensities after addition of small quantities of K+ and Mg2+ to the hemolysates were significantly larger than that expected from dilution of the sample, implying that the additions have resulted in the release of formerly invisible sodium. Furthermore, the intensities following the addition of K+ were significantly larger than those after the addition of Li+ and choline+. This signifies a specificity of the association sites at which sodium has decreased visibility. Increased T1 values following the additions confirmed the release of bound sodium. © 1989 Academic Press, Inc.

OriginalsprogEngelsk
TidsskriftMagnetic Resonance in Medicine
Vol/bind10
Udgave nummer3
Sider (fra-til)388-398
Antal sider11
ISSN0740-3194
DOI
StatusUdgivet - 1. jan. 1989

Fingeraftryk

Choline

Citer dette

@article{adb461ddeab84c588602a9e37e4826bc,
title = "Assessment of the NMR visibility of intraerythrocytic sodium by flame photometric and ion‐competitive studies",
abstract = "23Na nuclear magnetic resonance (NMR) studies of intraerythrocytic sodium concentrations were performed at 37°C and the accuracy of the method was evaluated through concomitant emission flame photometric analysis. NMR visibility of intraerythrocytic sodium was estimated to be 75 ± 3{\%}. Verification of the presence of an NMR invisible intraerythrocytic sodium pool and assessment of the mechanisms explaining this invisibility was achieved by measurement of 23Na NMR signal intensities and T1 relaxation times of erythrocyte hemolysate during ion‐competitive sodium release from association sites. Signal intensities after addition of small quantities of K+ and Mg2+ to the hemolysates were significantly larger than that expected from dilution of the sample, implying that the additions have resulted in the release of formerly invisible sodium. Furthermore, the intensities following the addition of K+ were significantly larger than those after the addition of Li+ and choline+. This signifies a specificity of the association sites at which sodium has decreased visibility. Increased T1 values following the additions confirmed the release of bound sodium. {\circledC} 1989 Academic Press, Inc.",
author = "Henrik Nissen and Jacobsen, {Jens Peter} and Mogens H{\o}rder",
year = "1989",
month = "1",
day = "1",
doi = "10.1002/mrm.1910100310",
language = "English",
volume = "10",
pages = "388--398",
journal = "Magnetic Resonance in Medicine",
issn = "0740-3194",
number = "3",

}

Assessment of the NMR visibility of intraerythrocytic sodium by flame photometric and ion‐competitive studies. / Nissen, Henrik; Jacobsen, Jens Peter; Hørder, Mogens.

I: Magnetic Resonance in Medicine, Bind 10, Nr. 3, 01.01.1989, s. 388-398.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Assessment of the NMR visibility of intraerythrocytic sodium by flame photometric and ion‐competitive studies

AU - Nissen, Henrik

AU - Jacobsen, Jens Peter

AU - Hørder, Mogens

PY - 1989/1/1

Y1 - 1989/1/1

N2 - 23Na nuclear magnetic resonance (NMR) studies of intraerythrocytic sodium concentrations were performed at 37°C and the accuracy of the method was evaluated through concomitant emission flame photometric analysis. NMR visibility of intraerythrocytic sodium was estimated to be 75 ± 3%. Verification of the presence of an NMR invisible intraerythrocytic sodium pool and assessment of the mechanisms explaining this invisibility was achieved by measurement of 23Na NMR signal intensities and T1 relaxation times of erythrocyte hemolysate during ion‐competitive sodium release from association sites. Signal intensities after addition of small quantities of K+ and Mg2+ to the hemolysates were significantly larger than that expected from dilution of the sample, implying that the additions have resulted in the release of formerly invisible sodium. Furthermore, the intensities following the addition of K+ were significantly larger than those after the addition of Li+ and choline+. This signifies a specificity of the association sites at which sodium has decreased visibility. Increased T1 values following the additions confirmed the release of bound sodium. © 1989 Academic Press, Inc.

AB - 23Na nuclear magnetic resonance (NMR) studies of intraerythrocytic sodium concentrations were performed at 37°C and the accuracy of the method was evaluated through concomitant emission flame photometric analysis. NMR visibility of intraerythrocytic sodium was estimated to be 75 ± 3%. Verification of the presence of an NMR invisible intraerythrocytic sodium pool and assessment of the mechanisms explaining this invisibility was achieved by measurement of 23Na NMR signal intensities and T1 relaxation times of erythrocyte hemolysate during ion‐competitive sodium release from association sites. Signal intensities after addition of small quantities of K+ and Mg2+ to the hemolysates were significantly larger than that expected from dilution of the sample, implying that the additions have resulted in the release of formerly invisible sodium. Furthermore, the intensities following the addition of K+ were significantly larger than those after the addition of Li+ and choline+. This signifies a specificity of the association sites at which sodium has decreased visibility. Increased T1 values following the additions confirmed the release of bound sodium. © 1989 Academic Press, Inc.

UR - http://www.scopus.com/inward/record.url?scp=0024328363&partnerID=8YFLogxK

U2 - 10.1002/mrm.1910100310

DO - 10.1002/mrm.1910100310

M3 - Journal article

C2 - 2733594

AN - SCOPUS:0024328363

VL - 10

SP - 388

EP - 398

JO - Magnetic Resonance in Medicine

JF - Magnetic Resonance in Medicine

SN - 0740-3194

IS - 3

ER -