Aptamers as a model for functional evaluation of LNA and 2'-amino LNA

Frank J. Hernandez, Neerja Kalra, Jesper Wengel, Birte Vester

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

The affinity change upon incorporation of LNA and 2′-amino-LNA monomers into an avidin binding DNA aptamer is described. The kinetic profile of selected modified-aptamer was obtained by surface plasmon resonance experiments and compared with the profile of the parent unmodified DNA aptamer. We report significant improvement of avidin binding affinity by the incorporation of single LNA modifications into the aptamer, and successful incorporation of 2′-amino LNA as a novel monomer in aptamers with potential function as carrier unit for additional molecular entities.
OriginalsprogEngelsk
TidsskriftBioorganic & Medicinal Chemistry Letters
Vol/bind19
Udgave nummer23
Sider (fra-til)6585-6587
Antal sider3
ISSN0960-894X
DOI
StatusUdgivet - 2009

Fingeraftryk

Nucleotide Aptamers
Avidin
Monomers
Surface Plasmon Resonance
Surface plasmon resonance
Kinetics
Experiments
amino-LNA

Citer dette

Hernandez, Frank J. ; Kalra, Neerja ; Wengel, Jesper ; Vester, Birte. / Aptamers as a model for functional evaluation of LNA and 2'-amino LNA. I: Bioorganic & Medicinal Chemistry Letters. 2009 ; Bind 19, Nr. 23. s. 6585-6587.
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Aptamers as a model for functional evaluation of LNA and 2'-amino LNA. / Hernandez, Frank J.; Kalra, Neerja; Wengel, Jesper; Vester, Birte.

I: Bioorganic & Medicinal Chemistry Letters, Bind 19, Nr. 23, 2009, s. 6585-6587.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Aptamers as a model for functional evaluation of LNA and 2'-amino LNA

AU - Hernandez, Frank J.

AU - Kalra, Neerja

AU - Wengel, Jesper

AU - Vester, Birte

PY - 2009

Y1 - 2009

N2 - The affinity change upon incorporation of LNA and 2′-amino-LNA monomers into an avidin binding DNA aptamer is described. The kinetic profile of selected modified-aptamer was obtained by surface plasmon resonance experiments and compared with the profile of the parent unmodified DNA aptamer. We report significant improvement of avidin binding affinity by the incorporation of single LNA modifications into the aptamer, and successful incorporation of 2′-amino LNA as a novel monomer in aptamers with potential function as carrier unit for additional molecular entities.

AB - The affinity change upon incorporation of LNA and 2′-amino-LNA monomers into an avidin binding DNA aptamer is described. The kinetic profile of selected modified-aptamer was obtained by surface plasmon resonance experiments and compared with the profile of the parent unmodified DNA aptamer. We report significant improvement of avidin binding affinity by the incorporation of single LNA modifications into the aptamer, and successful incorporation of 2′-amino LNA as a novel monomer in aptamers with potential function as carrier unit for additional molecular entities.

U2 - 10.1016/j.bmcl.2009.10.039

DO - 10.1016/j.bmcl.2009.10.039

M3 - Journal article

VL - 19

SP - 6585

EP - 6587

JO - Bioorganic & Medicinal Chemistry Letters

JF - Bioorganic & Medicinal Chemistry Letters

SN - 0960-894X

IS - 23

ER -