Abstract
Ultra High Performance Liquid Chromatography (UHPLC) pushes the limits of feasible column designs by allowing higher operational pressure. Migrating from nano-HPLC to nano-UHPLC and coupling directly to a mass spectrometer requires alterations to the setup to allow the increase in pressure. We systematically evaluate experimental parameters such as column dimensions and various stationary phases and how they apply to proteomic analysis.
| Bidragets oversatte titel | Anvendelse af online nano-HPLC til proteomics |
|---|---|
| Originalsprog | Engelsk |
| Publikationsdato | 6. jun. 2011 |
| Antal sider | 1 |
| Status | Udgivet - 6. jun. 2011 |
| Begivenhed | 59th American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics - Denver, CO, USA Varighed: 5. jun. 2011 → 9. jun. 2011 Konferencens nummer: 59 |
Konference
| Konference | 59th American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics |
|---|---|
| Nummer | 59 |
| Land/Område | USA |
| By | Denver, CO |
| Periode | 05/06/2011 → 09/06/2011 |
Emneord
- Proteomics
- LC-MS/MS
- HPLC