Apolipoprotein E polymorphism in India

high APOE*E3 allele frequency in Ramgarhia of Punjab

P Singh, M Singh, Lars Ulrik Gerdes, S S Mastana

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    High resolution two dimensional gel electrophoresis with the combination of isoelectric focusing (IEF) and density gradient sodium dodecyl-polyacrylamide gel electrophoresis (DG-PAGE) have been employed to investigate the distribution of APOE in Ramgarhia (n = 80) and Ramdasia (n = 70) of Punjab, India. Three alleles APOE*E2, APOE*E3 and APOE*E4 were observed in Ramgarhia and Ramdasia with the frequencies of 0.031, 0.913, 0.056 and 0.043, 0.886 and 0.071, respectively. Higher heterozygosity (20.8%) in Ramdasia reflects greater variation at the APOE locus. The APOE*E3 allele is found to be the highest (0.913) in Ramgarhia in comparison to forty-one populations of the world. A decreasing cline from south to north was evident for *E2 and *E4 allele frequencies (y = -0.002x + 0.141, r = 0.78 and y = -0.004x + 0.229, r = 0.83, respectively, and an increasing cline for the *E3 allele towards north was observed (y = 0.006x + 0.629, r = 0.82) in Asia.

    OriginalsprogEngelsk
    TidsskriftAnthropologischer Anzeiger
    Vol/bind59
    Udgave nummer1
    Sider (fra-til)27-34
    Antal sider8
    ISSN0003-5548
    StatusUdgivet - mar. 2001

    Fingeraftryk

    apolipoprotein E
    gene frequency
    allele
    polymorphism
    genetic polymorphism
    India
    alleles
    cline
    electrokinesis
    gel
    two-dimensional gel electrophoresis
    isoelectric focusing
    polyacrylamide gel electrophoresis
    heterozygosity
    sodium
    loci

    Citer dette

    Singh, P., Singh, M., Gerdes, L. U., & Mastana, S. S. (2001). Apolipoprotein E polymorphism in India: high APOE*E3 allele frequency in Ramgarhia of Punjab. Anthropologischer Anzeiger, 59(1), 27-34.
    Singh, P ; Singh, M ; Gerdes, Lars Ulrik ; Mastana, S S. / Apolipoprotein E polymorphism in India : high APOE*E3 allele frequency in Ramgarhia of Punjab. I: Anthropologischer Anzeiger. 2001 ; Bind 59, Nr. 1. s. 27-34.
    @article{6098934ddbd8472a94d49d5b17d12057,
    title = "Apolipoprotein E polymorphism in India: high APOE*E3 allele frequency in Ramgarhia of Punjab",
    abstract = "High resolution two dimensional gel electrophoresis with the combination of isoelectric focusing (IEF) and density gradient sodium dodecyl-polyacrylamide gel electrophoresis (DG-PAGE) have been employed to investigate the distribution of APOE in Ramgarhia (n = 80) and Ramdasia (n = 70) of Punjab, India. Three alleles APOE*E2, APOE*E3 and APOE*E4 were observed in Ramgarhia and Ramdasia with the frequencies of 0.031, 0.913, 0.056 and 0.043, 0.886 and 0.071, respectively. Higher heterozygosity (20.8{\%}) in Ramdasia reflects greater variation at the APOE locus. The APOE*E3 allele is found to be the highest (0.913) in Ramgarhia in comparison to forty-one populations of the world. A decreasing cline from south to north was evident for *E2 and *E4 allele frequencies (y = -0.002x + 0.141, r = 0.78 and y = -0.004x + 0.229, r = 0.83, respectively, and an increasing cline for the *E3 allele towards north was observed (y = 0.006x + 0.629, r = 0.82) in Asia.",
    keywords = "Adult, Alleles, Apolipoprotein E2, Apolipoprotein E3, Apolipoprotein E4, Apolipoproteins E, Developing Countries, Female, Gene Frequency, Genetics, Population, Humans, India, Male, Middle Aged, Phenotype",
    author = "P Singh and M Singh and Gerdes, {Lars Ulrik} and Mastana, {S S}",
    year = "2001",
    month = "3",
    language = "English",
    volume = "59",
    pages = "27--34",
    journal = "Anthropologischer Anzeiger",
    issn = "0003-5548",
    publisher = "E.Schweizerbart'sche Verlagsbuchhandlung",
    number = "1",

    }

    Singh, P, Singh, M, Gerdes, LU & Mastana, SS 2001, 'Apolipoprotein E polymorphism in India: high APOE*E3 allele frequency in Ramgarhia of Punjab', Anthropologischer Anzeiger, bind 59, nr. 1, s. 27-34.

    Apolipoprotein E polymorphism in India : high APOE*E3 allele frequency in Ramgarhia of Punjab. / Singh, P; Singh, M; Gerdes, Lars Ulrik; Mastana, S S.

    I: Anthropologischer Anzeiger, Bind 59, Nr. 1, 03.2001, s. 27-34.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - Apolipoprotein E polymorphism in India

    T2 - high APOE*E3 allele frequency in Ramgarhia of Punjab

    AU - Singh, P

    AU - Singh, M

    AU - Gerdes, Lars Ulrik

    AU - Mastana, S S

    PY - 2001/3

    Y1 - 2001/3

    N2 - High resolution two dimensional gel electrophoresis with the combination of isoelectric focusing (IEF) and density gradient sodium dodecyl-polyacrylamide gel electrophoresis (DG-PAGE) have been employed to investigate the distribution of APOE in Ramgarhia (n = 80) and Ramdasia (n = 70) of Punjab, India. Three alleles APOE*E2, APOE*E3 and APOE*E4 were observed in Ramgarhia and Ramdasia with the frequencies of 0.031, 0.913, 0.056 and 0.043, 0.886 and 0.071, respectively. Higher heterozygosity (20.8%) in Ramdasia reflects greater variation at the APOE locus. The APOE*E3 allele is found to be the highest (0.913) in Ramgarhia in comparison to forty-one populations of the world. A decreasing cline from south to north was evident for *E2 and *E4 allele frequencies (y = -0.002x + 0.141, r = 0.78 and y = -0.004x + 0.229, r = 0.83, respectively, and an increasing cline for the *E3 allele towards north was observed (y = 0.006x + 0.629, r = 0.82) in Asia.

    AB - High resolution two dimensional gel electrophoresis with the combination of isoelectric focusing (IEF) and density gradient sodium dodecyl-polyacrylamide gel electrophoresis (DG-PAGE) have been employed to investigate the distribution of APOE in Ramgarhia (n = 80) and Ramdasia (n = 70) of Punjab, India. Three alleles APOE*E2, APOE*E3 and APOE*E4 were observed in Ramgarhia and Ramdasia with the frequencies of 0.031, 0.913, 0.056 and 0.043, 0.886 and 0.071, respectively. Higher heterozygosity (20.8%) in Ramdasia reflects greater variation at the APOE locus. The APOE*E3 allele is found to be the highest (0.913) in Ramgarhia in comparison to forty-one populations of the world. A decreasing cline from south to north was evident for *E2 and *E4 allele frequencies (y = -0.002x + 0.141, r = 0.78 and y = -0.004x + 0.229, r = 0.83, respectively, and an increasing cline for the *E3 allele towards north was observed (y = 0.006x + 0.629, r = 0.82) in Asia.

    KW - Adult

    KW - Alleles

    KW - Apolipoprotein E2

    KW - Apolipoprotein E3

    KW - Apolipoprotein E4

    KW - Apolipoproteins E

    KW - Developing Countries

    KW - Female

    KW - Gene Frequency

    KW - Genetics, Population

    KW - Humans

    KW - India

    KW - Male

    KW - Middle Aged

    KW - Phenotype

    M3 - Journal article

    VL - 59

    SP - 27

    EP - 34

    JO - Anthropologischer Anzeiger

    JF - Anthropologischer Anzeiger

    SN - 0003-5548

    IS - 1

    ER -