Anti-Müllerian hormone remains highly expressed in human cumulus cells during the final stages of folliculogenesis

M L Grøndahl, M Eilsø Nielsen, M B Dal Canto, R Fadini, I A Rasmussen, L G Westergaard, S G Kristensen, Claus Yding Andersen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

This study evaluated whether anti-Müllerian hormone (AMH) was differentially expressed in cumulus (CC) and granulosa (GC) cells from large antral and pre-ovulatory follicles collected from individual follicles in women undergoing in-vitro maturation (IVM) or IVF treatment. Expression studies of AMH, AMH receptor 2, FSH receptor, aromatase and androgen receptor were performed in CC in IVM patients where cumulus-oocyte-complex had expanded, CC in IVM patients where cumulus-oocyte-complex remained compacted, GC from immature follicles and CC and GC from IVF patients. Microarray data on corresponding GC and CC from follicles from IVF patients was included. AMH expression was significantly higher in CC than in GC from both mature and immature follicles and in CC from immature follicles than in CC from pre-ovulatory follicles from IVF patients (P <0.05). AMH expression was significantly higher in CC that remained compacted compared with those that had expanded (P <0.008). AMH was correlated to the expression of FSH receptor, androgen receptor and AMH receptor 2 but not to aromatase expression. The expression pattern of AMH receptor 2 reflected that of AMH. AMH may exert intrafollicular functions even in human large antral and pre-ovulatory follicles and may be related to follicular health.
OriginalsprogEngelsk
TidsskriftReproductive BioMedicine Online
Vol/bind22
Udgave nummer4
Sider (fra-til)389-98
Antal sider10
ISSN1472-6483
DOI
StatusUdgivet - 2011

Fingeraftryk

Hormones
FSH Receptors
Androgen Receptors
Oocytes
Granulosa Cells
Health
In Vitro Techniques

Citer dette

Grøndahl, M. L., Nielsen, M. E., Dal Canto, M. B., Fadini, R., Rasmussen, I. A., Westergaard, L. G., ... Andersen, C. Y. (2011). Anti-Müllerian hormone remains highly expressed in human cumulus cells during the final stages of folliculogenesis. Reproductive BioMedicine Online, 22(4), 389-98. https://doi.org/10.1016/j.rbmo.2010.12.005
Grøndahl, M L ; Nielsen, M Eilsø ; Dal Canto, M B ; Fadini, R ; Rasmussen, I A ; Westergaard, L G ; Kristensen, S G ; Andersen, Claus Yding. / Anti-Müllerian hormone remains highly expressed in human cumulus cells during the final stages of folliculogenesis. I: Reproductive BioMedicine Online. 2011 ; Bind 22, Nr. 4. s. 389-98.
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title = "Anti-M{\"u}llerian hormone remains highly expressed in human cumulus cells during the final stages of folliculogenesis",
abstract = "This study evaluated whether anti-M{\"u}llerian hormone (AMH) was differentially expressed in cumulus (CC) and granulosa (GC) cells from large antral and pre-ovulatory follicles collected from individual follicles in women undergoing in-vitro maturation (IVM) or IVF treatment. Expression studies of AMH, AMH receptor 2, FSH receptor, aromatase and androgen receptor were performed in CC in IVM patients where cumulus-oocyte-complex had expanded, CC in IVM patients where cumulus-oocyte-complex remained compacted, GC from immature follicles and CC and GC from IVF patients. Microarray data on corresponding GC and CC from follicles from IVF patients was included. AMH expression was significantly higher in CC than in GC from both mature and immature follicles and in CC from immature follicles than in CC from pre-ovulatory follicles from IVF patients (P <0.05). AMH expression was significantly higher in CC that remained compacted compared with those that had expanded (P <0.008). AMH was correlated to the expression of FSH receptor, androgen receptor and AMH receptor 2 but not to aromatase expression. The expression pattern of AMH receptor 2 reflected that of AMH. AMH may exert intrafollicular functions even in human large antral and pre-ovulatory follicles and may be related to follicular health.",
keywords = "Anti-Mullerian Hormone, Aromatase, Blotting, Western, Cumulus Cells, DNA, Complementary, Electrophoresis, Polyacrylamide Gel, Female, Humans, Linear Models, Microarray Analysis, Ovarian Follicle, Polymerase Chain Reaction, Receptors, Androgen, Receptors, FSH, Receptors, Peptide, Receptors, Transforming Growth Factor beta, Reproductive Techniques, Assisted",
author = "Gr{\o}ndahl, {M L} and Nielsen, {M Eils{\o}} and {Dal Canto}, {M B} and R Fadini and Rasmussen, {I A} and Westergaard, {L G} and Kristensen, {S G} and Andersen, {Claus Yding}",
note = "Copyright {\circledC} 2010 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.",
year = "2011",
doi = "10.1016/j.rbmo.2010.12.005",
language = "English",
volume = "22",
pages = "389--98",
journal = "Reproductive BioMedicine Online",
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Grøndahl, ML, Nielsen, ME, Dal Canto, MB, Fadini, R, Rasmussen, IA, Westergaard, LG, Kristensen, SG & Andersen, CY 2011, 'Anti-Müllerian hormone remains highly expressed in human cumulus cells during the final stages of folliculogenesis', Reproductive BioMedicine Online, bind 22, nr. 4, s. 389-98. https://doi.org/10.1016/j.rbmo.2010.12.005

Anti-Müllerian hormone remains highly expressed in human cumulus cells during the final stages of folliculogenesis. / Grøndahl, M L; Nielsen, M Eilsø; Dal Canto, M B; Fadini, R; Rasmussen, I A; Westergaard, L G; Kristensen, S G; Andersen, Claus Yding.

I: Reproductive BioMedicine Online, Bind 22, Nr. 4, 2011, s. 389-98.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Anti-Müllerian hormone remains highly expressed in human cumulus cells during the final stages of folliculogenesis

AU - Grøndahl, M L

AU - Nielsen, M Eilsø

AU - Dal Canto, M B

AU - Fadini, R

AU - Rasmussen, I A

AU - Westergaard, L G

AU - Kristensen, S G

AU - Andersen, Claus Yding

N1 - Copyright © 2010 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

PY - 2011

Y1 - 2011

N2 - This study evaluated whether anti-Müllerian hormone (AMH) was differentially expressed in cumulus (CC) and granulosa (GC) cells from large antral and pre-ovulatory follicles collected from individual follicles in women undergoing in-vitro maturation (IVM) or IVF treatment. Expression studies of AMH, AMH receptor 2, FSH receptor, aromatase and androgen receptor were performed in CC in IVM patients where cumulus-oocyte-complex had expanded, CC in IVM patients where cumulus-oocyte-complex remained compacted, GC from immature follicles and CC and GC from IVF patients. Microarray data on corresponding GC and CC from follicles from IVF patients was included. AMH expression was significantly higher in CC than in GC from both mature and immature follicles and in CC from immature follicles than in CC from pre-ovulatory follicles from IVF patients (P <0.05). AMH expression was significantly higher in CC that remained compacted compared with those that had expanded (P <0.008). AMH was correlated to the expression of FSH receptor, androgen receptor and AMH receptor 2 but not to aromatase expression. The expression pattern of AMH receptor 2 reflected that of AMH. AMH may exert intrafollicular functions even in human large antral and pre-ovulatory follicles and may be related to follicular health.

AB - This study evaluated whether anti-Müllerian hormone (AMH) was differentially expressed in cumulus (CC) and granulosa (GC) cells from large antral and pre-ovulatory follicles collected from individual follicles in women undergoing in-vitro maturation (IVM) or IVF treatment. Expression studies of AMH, AMH receptor 2, FSH receptor, aromatase and androgen receptor were performed in CC in IVM patients where cumulus-oocyte-complex had expanded, CC in IVM patients where cumulus-oocyte-complex remained compacted, GC from immature follicles and CC and GC from IVF patients. Microarray data on corresponding GC and CC from follicles from IVF patients was included. AMH expression was significantly higher in CC than in GC from both mature and immature follicles and in CC from immature follicles than in CC from pre-ovulatory follicles from IVF patients (P <0.05). AMH expression was significantly higher in CC that remained compacted compared with those that had expanded (P <0.008). AMH was correlated to the expression of FSH receptor, androgen receptor and AMH receptor 2 but not to aromatase expression. The expression pattern of AMH receptor 2 reflected that of AMH. AMH may exert intrafollicular functions even in human large antral and pre-ovulatory follicles and may be related to follicular health.

KW - Anti-Mullerian Hormone

KW - Aromatase

KW - Blotting, Western

KW - Cumulus Cells

KW - DNA, Complementary

KW - Electrophoresis, Polyacrylamide Gel

KW - Female

KW - Humans

KW - Linear Models

KW - Microarray Analysis

KW - Ovarian Follicle

KW - Polymerase Chain Reaction

KW - Receptors, Androgen

KW - Receptors, FSH

KW - Receptors, Peptide

KW - Receptors, Transforming Growth Factor beta

KW - Reproductive Techniques, Assisted

U2 - 10.1016/j.rbmo.2010.12.005

DO - 10.1016/j.rbmo.2010.12.005

M3 - Journal article

VL - 22

SP - 389

EP - 398

JO - Reproductive BioMedicine Online

JF - Reproductive BioMedicine Online

SN - 1472-6483

IS - 4

ER -