Anti-inflammatory liposomes have no impact on liver regeneration in rats

Betina Norman Jepsen, Kasper Jarlhelt Andersen, Anders Riegels Knudsen, Jens Randel Nyengaard, Stephen Hamilton-Dutoit, Pia Svendsen, Anders Etzerodt, Holger Jon Møller, S. K. Moestrup, Jonas Heilskov Graversen, Frank Viborg Mortensen

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    232 Downloads (Pure)

    Abstract

    Introduction: Surgical resection is the gold standard in treatment of hepatic malignancies, giving the patient the best chance to be cured. The liver has a unique capacity to regenerate. However, an inflammatory response occurs during resection, in part mediated by Kupffer cells, that influences the speed of regeneration. The aim of this study was to investigate the effect of a Kupffer cell targeted anti-inflammatory treatment on liver regeneration in rats. Methods: Two sets of animals, each including four groups of eight rats, were included. Paired groups from each set received treatment with placebo, low dose dexamethasone, high dose dexamethasone or low dose anti-CD163 dexamethasone. Subsequently, the rats underwent 70% partial hepatectomy. The two sets were evaluated on postoperative day 2 or 5, respectively. Blood was drawn for circulating markers of inflammation and liver cell damage; liver tissue was sampled for analysis of regeneration rate and proliferation index. Results: The high dose dexamethasone group had significantly lower body and liver weight than the placebo and anti-CD163-dex groups. There were no differences in liver regeneration rates between groups. Hepatocyte proliferation was completed faster in the placebo group, although this was not significant. The anti-CD163-dex group showed increased blood levels of albumin and alanine aminotransferase and a diminished inflammatory response in terms of significantly reduced haptoglobin, α2-macroglobulin and Interleukine-6. Conclusion: Low dose dexamethasone targeted to Kupffer cells does not affect histological liver cell regeneration after 70% hepatectomy in rats, but reduces the inflammatory response judged by circulating markers of inflammation.

    OriginalsprogEngelsk
    TidsskriftAnnals of Medicine and Surgery
    Vol/bind4
    Udgave nummer4
    Sider (fra-til)452-461
    ISSN2049-0801
    DOI
    StatusUdgivet - dec. 2015

    Bibliografisk note

    0 26779334

    Emneord

    • Liver regeneration CD-163-Dexamethasone Hepatic surgery ISCHEMIA-REPERFUSION INJURY SCAVENGER RECEPTOR CD163 100 CONSECUTIVE PATIENTS DESIGN-BASED STEREOLOGY HEPATIC ISCHEMIA PARTIAL-HEPATECTOMY COLORECTAL-CANCER RESECTION DEXAMETHASONE MACROPHAGES

    Fingeraftryk

    Dyk ned i forskningsemnerne om 'Anti-inflammatory liposomes have no impact on liver regeneration in rats'. Sammen danner de et unikt fingeraftryk.

    Citationsformater