The tepals of 28 Chilean Alstroemeria species were investigated by HPLC for their content of anthocyanins. The investigation revealed the presence of at least seven anthocyanins of which six were identified. The anthocyanins were isolated from the cultivars 'Regina' and 'Cana' and identified as 6-hydroxydelphinidin 3-rutinoside, 6-hydroxycyanidin 3-rutinoside, delphinidin 3-rutinoside, cyanidin 3-rutinoside, delphinidin 3-malonylglucoside and cyanidin 3-malonylglucoside. The position of the malonyl group in the acylated anthocyanins could not be determined due to their instability and low amounts. Most of the Alstroemeria species investigated can be placed into three groups regarding their contents of major pigments. The use of anthocyanins as taxonomic markers in Alstroemeria is discussed.