Annexins induce curvature on free-edge membranes displaying distinct morphologies

Theresa Louise Boye, Jonas Camillus Jeppesen, Kenji Maeda, Weria Pezeshkian, Vita Solovyeva, Jesper Nylandsted, Adam Cohen Simonsen*

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Resumé

Annexins are a family of proteins characterized by their ability to bind anionic membranes in response to Ca2+-Activation. They are involved in a multitude of cellular functions including vesiculation and membrane repair. Here, we investigate the effect of nine annexins (ANXA1-ANXA7, ANXA11, ANXA13) on negatively charged double supported membrane patches with free edges. We find that annexin members can be classified according to the membrane morphology they induce and matching a dendrogam of the annexin family based on full amino acid sequences. ANXA1 and ANXA2 induce membrane folding and blebbing initiated from membrane structural defects inside patches while ANXA6 induces membrane folding originating both from defects and from the membrane edges. ANXA4 and ANXA5 induce cooperative roll-up of the membrane starting from free edges, producing large rolls. In contrast, ANXA3 and ANXA13 roll the membrane in a fragmented manner producing multiple thin rolls. In addition to rolling, ANXA7 and ANXA11 are characterized by their ability to form fluid lenses localized between the membrane leaflets. A shared feature necessary for generating these morphologies is the ability to induce membrane curvature on free edged anionic membranes. Consequently, induction of membrane curvature may be a significant property of the annexin protein family that is important for their function.

OriginalsprogEngelsk
Artikelnummer10309
TidsskriftScientific Reports
Vol/bind8
Antal sider14
ISSN2045-2322
DOI
StatusUdgivet - jul. 2018

Fingeraftryk

Membranes
Lenses
Proteins

Citer dette

Boye, T. L., Jeppesen, J. C., Maeda, K., Pezeshkian, W., Solovyeva, V., Nylandsted, J., & Simonsen, A. C. (2018). Annexins induce curvature on free-edge membranes displaying distinct morphologies. Scientific Reports, 8, [10309]. https://doi.org/10.1038/s41598-018-28481-z
Boye, Theresa Louise ; Jeppesen, Jonas Camillus ; Maeda, Kenji ; Pezeshkian, Weria ; Solovyeva, Vita ; Nylandsted, Jesper ; Simonsen, Adam Cohen. / Annexins induce curvature on free-edge membranes displaying distinct morphologies. I: Scientific Reports. 2018 ; Bind 8.
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abstract = "Annexins are a family of proteins characterized by their ability to bind anionic membranes in response to Ca2+-Activation. They are involved in a multitude of cellular functions including vesiculation and membrane repair. Here, we investigate the effect of nine annexins (ANXA1-ANXA7, ANXA11, ANXA13) on negatively charged double supported membrane patches with free edges. We find that annexin members can be classified according to the membrane morphology they induce and matching a dendrogam of the annexin family based on full amino acid sequences. ANXA1 and ANXA2 induce membrane folding and blebbing initiated from membrane structural defects inside patches while ANXA6 induces membrane folding originating both from defects and from the membrane edges. ANXA4 and ANXA5 induce cooperative roll-up of the membrane starting from free edges, producing large rolls. In contrast, ANXA3 and ANXA13 roll the membrane in a fragmented manner producing multiple thin rolls. In addition to rolling, ANXA7 and ANXA11 are characterized by their ability to form fluid lenses localized between the membrane leaflets. A shared feature necessary for generating these morphologies is the ability to induce membrane curvature on free edged anionic membranes. Consequently, induction of membrane curvature may be a significant property of the annexin protein family that is important for their function.",
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Boye, TL, Jeppesen, JC, Maeda, K, Pezeshkian, W, Solovyeva, V, Nylandsted, J & Simonsen, AC 2018, 'Annexins induce curvature on free-edge membranes displaying distinct morphologies', Scientific Reports, bind 8, 10309. https://doi.org/10.1038/s41598-018-28481-z

Annexins induce curvature on free-edge membranes displaying distinct morphologies. / Boye, Theresa Louise; Jeppesen, Jonas Camillus; Maeda, Kenji; Pezeshkian, Weria; Solovyeva, Vita; Nylandsted, Jesper; Simonsen, Adam Cohen.

I: Scientific Reports, Bind 8, 10309, 07.2018.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Annexins induce curvature on free-edge membranes displaying distinct morphologies

AU - Boye, Theresa Louise

AU - Jeppesen, Jonas Camillus

AU - Maeda, Kenji

AU - Pezeshkian, Weria

AU - Solovyeva, Vita

AU - Nylandsted, Jesper

AU - Simonsen, Adam Cohen

PY - 2018/7

Y1 - 2018/7

N2 - Annexins are a family of proteins characterized by their ability to bind anionic membranes in response to Ca2+-Activation. They are involved in a multitude of cellular functions including vesiculation and membrane repair. Here, we investigate the effect of nine annexins (ANXA1-ANXA7, ANXA11, ANXA13) on negatively charged double supported membrane patches with free edges. We find that annexin members can be classified according to the membrane morphology they induce and matching a dendrogam of the annexin family based on full amino acid sequences. ANXA1 and ANXA2 induce membrane folding and blebbing initiated from membrane structural defects inside patches while ANXA6 induces membrane folding originating both from defects and from the membrane edges. ANXA4 and ANXA5 induce cooperative roll-up of the membrane starting from free edges, producing large rolls. In contrast, ANXA3 and ANXA13 roll the membrane in a fragmented manner producing multiple thin rolls. In addition to rolling, ANXA7 and ANXA11 are characterized by their ability to form fluid lenses localized between the membrane leaflets. A shared feature necessary for generating these morphologies is the ability to induce membrane curvature on free edged anionic membranes. Consequently, induction of membrane curvature may be a significant property of the annexin protein family that is important for their function.

AB - Annexins are a family of proteins characterized by their ability to bind anionic membranes in response to Ca2+-Activation. They are involved in a multitude of cellular functions including vesiculation and membrane repair. Here, we investigate the effect of nine annexins (ANXA1-ANXA7, ANXA11, ANXA13) on negatively charged double supported membrane patches with free edges. We find that annexin members can be classified according to the membrane morphology they induce and matching a dendrogam of the annexin family based on full amino acid sequences. ANXA1 and ANXA2 induce membrane folding and blebbing initiated from membrane structural defects inside patches while ANXA6 induces membrane folding originating both from defects and from the membrane edges. ANXA4 and ANXA5 induce cooperative roll-up of the membrane starting from free edges, producing large rolls. In contrast, ANXA3 and ANXA13 roll the membrane in a fragmented manner producing multiple thin rolls. In addition to rolling, ANXA7 and ANXA11 are characterized by their ability to form fluid lenses localized between the membrane leaflets. A shared feature necessary for generating these morphologies is the ability to induce membrane curvature on free edged anionic membranes. Consequently, induction of membrane curvature may be a significant property of the annexin protein family that is important for their function.

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DO - 10.1038/s41598-018-28481-z

M3 - Journal article

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AN - SCOPUS:85049859793

VL - 8

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

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Boye TL, Jeppesen JC, Maeda K, Pezeshkian W, Solovyeva V, Nylandsted J et al. Annexins induce curvature on free-edge membranes displaying distinct morphologies. Scientific Reports. 2018 jul;8. 10309. https://doi.org/10.1038/s41598-018-28481-z