Annexin A7 is required for ESCRT III-mediated plasma membrane repair

Stine Lauritzen Sønder, Theresa Louise Boye, Regine Tölle, Jörn Dengjel, Kenji Maeda, Marja Jäättelä, Adam Cohen Simonsen, Jyoti K. Jaiswal, Jesper Nylandsted*

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

The plasma membrane of eukaryotic cells forms the essential barrier to the extracellular environment, and thus plasma membrane disruptions pose a fatal threat to cells. Here, using invasive breast cancer cells we show that the Ca 2+ - and phospholipid-binding protein annexin A7 is part of the plasma membrane repair response by enabling assembly of the endosomal sorting complex required for transport (ESCRT) III. Following injury to the plasma membrane and Ca 2+ flux into the cytoplasm, annexin A7 forms a complex with apoptosis linked gene-2 (ALG-2) to facilitate proper recruitment and binding of ALG-2 and ALG-2-interacting protein X (ALIX) to the damaged membrane. ALG-2 and ALIX assemble the ESCRT III complex, which helps excise and shed the damaged portion of the plasma membrane during wound healing. Our results reveal a novel function of annexin A7 – enabling plasma membrane repair by regulating ESCRT III-mediated shedding of injured plasma membrane.

OriginalsprogEngelsk
Artikelnummer6726
TidsskriftScientific Reports
Vol/bind9
Udgave nummer1
ISSN2045-2322
DOI
StatusUdgivet - 1. dec. 2019

Fingeraftryk

Annexin A7
Endosomal Sorting Complexes Required for Transport
Cell Membrane
Apoptosis
Eukaryotic Cells
Phospholipids
Proteins

Citer dette

Sønder, S. L., Boye, T. L., Tölle, R., Dengjel, J., Maeda, K., Jäättelä, M., ... Nylandsted, J. (2019). Annexin A7 is required for ESCRT III-mediated plasma membrane repair. Scientific Reports, 9(1), [6726]. https://doi.org/10.1038/s41598-019-43143-4
Sønder, Stine Lauritzen ; Boye, Theresa Louise ; Tölle, Regine ; Dengjel, Jörn ; Maeda, Kenji ; Jäättelä, Marja ; Simonsen, Adam Cohen ; Jaiswal, Jyoti K. ; Nylandsted, Jesper. / Annexin A7 is required for ESCRT III-mediated plasma membrane repair. I: Scientific Reports. 2019 ; Bind 9, Nr. 1.
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Sønder, SL, Boye, TL, Tölle, R, Dengjel, J, Maeda, K, Jäättelä, M, Simonsen, AC, Jaiswal, JK & Nylandsted, J 2019, 'Annexin A7 is required for ESCRT III-mediated plasma membrane repair', Scientific Reports, bind 9, nr. 1, 6726. https://doi.org/10.1038/s41598-019-43143-4

Annexin A7 is required for ESCRT III-mediated plasma membrane repair. / Sønder, Stine Lauritzen; Boye, Theresa Louise; Tölle, Regine; Dengjel, Jörn; Maeda, Kenji; Jäättelä, Marja; Simonsen, Adam Cohen; Jaiswal, Jyoti K.; Nylandsted, Jesper.

I: Scientific Reports, Bind 9, Nr. 1, 6726, 01.12.2019.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Annexin A7 is required for ESCRT III-mediated plasma membrane repair

AU - Sønder, Stine Lauritzen

AU - Boye, Theresa Louise

AU - Tölle, Regine

AU - Dengjel, Jörn

AU - Maeda, Kenji

AU - Jäättelä, Marja

AU - Simonsen, Adam Cohen

AU - Jaiswal, Jyoti K.

AU - Nylandsted, Jesper

PY - 2019/12/1

Y1 - 2019/12/1

N2 - The plasma membrane of eukaryotic cells forms the essential barrier to the extracellular environment, and thus plasma membrane disruptions pose a fatal threat to cells. Here, using invasive breast cancer cells we show that the Ca 2+ - and phospholipid-binding protein annexin A7 is part of the plasma membrane repair response by enabling assembly of the endosomal sorting complex required for transport (ESCRT) III. Following injury to the plasma membrane and Ca 2+ flux into the cytoplasm, annexin A7 forms a complex with apoptosis linked gene-2 (ALG-2) to facilitate proper recruitment and binding of ALG-2 and ALG-2-interacting protein X (ALIX) to the damaged membrane. ALG-2 and ALIX assemble the ESCRT III complex, which helps excise and shed the damaged portion of the plasma membrane during wound healing. Our results reveal a novel function of annexin A7 – enabling plasma membrane repair by regulating ESCRT III-mediated shedding of injured plasma membrane.

AB - The plasma membrane of eukaryotic cells forms the essential barrier to the extracellular environment, and thus plasma membrane disruptions pose a fatal threat to cells. Here, using invasive breast cancer cells we show that the Ca 2+ - and phospholipid-binding protein annexin A7 is part of the plasma membrane repair response by enabling assembly of the endosomal sorting complex required for transport (ESCRT) III. Following injury to the plasma membrane and Ca 2+ flux into the cytoplasm, annexin A7 forms a complex with apoptosis linked gene-2 (ALG-2) to facilitate proper recruitment and binding of ALG-2 and ALG-2-interacting protein X (ALIX) to the damaged membrane. ALG-2 and ALIX assemble the ESCRT III complex, which helps excise and shed the damaged portion of the plasma membrane during wound healing. Our results reveal a novel function of annexin A7 – enabling plasma membrane repair by regulating ESCRT III-mediated shedding of injured plasma membrane.

U2 - 10.1038/s41598-019-43143-4

DO - 10.1038/s41598-019-43143-4

M3 - Journal article

VL - 9

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

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Sønder SL, Boye TL, Tölle R, Dengjel J, Maeda K, Jäättelä M et al. Annexin A7 is required for ESCRT III-mediated plasma membrane repair. Scientific Reports. 2019 dec 1;9(1). 6726. https://doi.org/10.1038/s41598-019-43143-4