Annexin A4 and A6 induce membrane curvature and constriction during cell membrane repair

Theresa Louise Boye, Kenji Maeda, Weria Pezeshkian, Stine Lauritzen Sønder, Swantje Christin Haeger, Volker Gerke, Adam Cohen Simonsen, Jesper Nylandsted

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

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Resumé

Efficient cell membrane repair mechanisms are essential for maintaining membrane integrity and thus for cell life. Here we show that the Ca2+- and phospholipid-binding proteins annexin A4 and A6 are involved in plasma membrane repair and needed for rapid closure of micron-size holes. We demonstrate that annexin A4 binds to artificial membranes and generates curvature force initiated from free edges, whereas annexin A6 induces constriction force. In cells, plasma membrane injury and Ca2+ influx recruit annexin A4 to the vicinity of membrane wound edges where its homo-trimerization leads to membrane curvature near the edges. We propose that curvature force is utilized together with annexin A6-mediated constriction force to pull the wound edges together for eventual fusion. We show that annexin A4 can counteract various plasma membrane disruptions including holes of several micrometers indicating that induction of curvature force around wound edges is an early key event in cell membrane repair.

OriginalsprogEngelsk
Artikelnummer1623
TidsskriftNature Communications
Vol/bind8
Antal sider11
ISSN2041-1723
DOI
StatusUdgivet - 2017

Fingeraftryk

Annexin A4
Annexin A6
Cell membranes
Constriction
constrictions
Repair
curvature
Cell Membrane
membranes
Membranes
Wounds and Injuries
Phospholipids
Carrier Proteins
Fusion reactions
cells
integrity
closures
micrometers
induction
fusion

Citer dette

Boye, T. L., Maeda, K., Pezeshkian, W., Sønder, S. L., Haeger, S. C., Gerke, V., ... Nylandsted, J. (2017). Annexin A4 and A6 induce membrane curvature and constriction during cell membrane repair. Nature Communications, 8, [1623]. https://doi.org/10.1038/s41467-017-01743-6
Boye, Theresa Louise ; Maeda, Kenji ; Pezeshkian, Weria ; Sønder, Stine Lauritzen ; Haeger, Swantje Christin ; Gerke, Volker ; Simonsen, Adam Cohen ; Nylandsted, Jesper. / Annexin A4 and A6 induce membrane curvature and constriction during cell membrane repair. I: Nature Communications. 2017 ; Bind 8.
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Boye, TL, Maeda, K, Pezeshkian, W, Sønder, SL, Haeger, SC, Gerke, V, Simonsen, AC & Nylandsted, J 2017, 'Annexin A4 and A6 induce membrane curvature and constriction during cell membrane repair', Nature Communications, bind 8, 1623. https://doi.org/10.1038/s41467-017-01743-6

Annexin A4 and A6 induce membrane curvature and constriction during cell membrane repair. / Boye, Theresa Louise; Maeda, Kenji; Pezeshkian, Weria; Sønder, Stine Lauritzen; Haeger, Swantje Christin; Gerke, Volker; Simonsen, Adam Cohen; Nylandsted, Jesper.

I: Nature Communications, Bind 8, 1623, 2017.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Annexin A4 and A6 induce membrane curvature and constriction during cell membrane repair

AU - Boye, Theresa Louise

AU - Maeda, Kenji

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AU - Sønder, Stine Lauritzen

AU - Haeger, Swantje Christin

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AU - Simonsen, Adam Cohen

AU - Nylandsted, Jesper

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AB - Efficient cell membrane repair mechanisms are essential for maintaining membrane integrity and thus for cell life. Here we show that the Ca2+- and phospholipid-binding proteins annexin A4 and A6 are involved in plasma membrane repair and needed for rapid closure of micron-size holes. We demonstrate that annexin A4 binds to artificial membranes and generates curvature force initiated from free edges, whereas annexin A6 induces constriction force. In cells, plasma membrane injury and Ca2+ influx recruit annexin A4 to the vicinity of membrane wound edges where its homo-trimerization leads to membrane curvature near the edges. We propose that curvature force is utilized together with annexin A6-mediated constriction force to pull the wound edges together for eventual fusion. We show that annexin A4 can counteract various plasma membrane disruptions including holes of several micrometers indicating that induction of curvature force around wound edges is an early key event in cell membrane repair.

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