Angiotensin II Treatment Induces Reorganization and Changes in the Lateral Dynamics of Angiotensin II Type 1 Receptor in the Plasma Membrane Elucidated by Photoactivated Localization Microscopy Combined with Image Spatial Correlation Analysis

Camilla Andersen; Yenisleidy de las Mercedes Zulueta Díaz; Jakob Lavrsen Kure; Mathias Hessellund Eriksen; Adam Leslie Lovatt; Christoffer  Lagerholm; Sebastian  Morales; Simon  Sehayek; Thomas M.D  Sheard; Paul W.  Wiseman; Eva C. Arnspang

doi:10.1021/acs.analchem.2c02720

Angiotensin II Treatment Induces Reorganization and Changes in the Lateral Dynamics of Angiotensin II Type 1 Receptor in the Plasma Membrane Elucidated by Photoactivated Localization Microscopy Combined with Image Spatial Correlation Analysis

Camilla Andersen, Yenisleidy de las Mercedes Zulueta Díaz, Jakob Lavrsen Kure, Mathias Hessellund Eriksen, Adam Leslie Lovatt, Christoffer Lagerholm , Sebastian Morales , Simon Sehayek , Thomas M.D Sheard , Paul W. Wiseman , Eva C. Arnspang^*

^*Kontaktforfatter

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Abstrakt

The mechanisms by which angiotensin II type 1 receptor is distributed and the diffusional pattern in the plasma membrane (PM) remain unclear, despite their crucial role in cardiovascular homeostasis. In this work, we obtained quantitative information of angiotensin II type 1 receptor (AT1R) lateral dynamics as well as changes in the diffusion properties after stimulation with ligands in living cells using photoactivated localization microscopy (PALM) combined with image spatial-temporal correlation analysis. To study the organization of the receptor at the nanoscale, expansion microscopy (ExM) combined with PALM was performed. This study revealed that AT1R lateral diffusion increased after binding to angiotensin II (Ang II) and the receptor diffusion was transiently confined in the PM. In addition, ExM revealed that AT1R formed nanoclusters at the PM and the cluster size significantly decreased after Ang II treatment. Taking these results together suggest that Ang II binding and activation cause reorganization and changes in the dynamics of AT1R at the PM.

Originalsprog	Engelsk
Tidsskrift	Analytical Chemistry
Vol/bind	95
Udgave nummer	2
Sider (fra-til)	730–738
ISSN	0003-2700
DOI	https://doi.org/10.1021/acs.analchem.2c02720
Status	Udgivet - 17. jan. 2023

Dokumenter og links

10.1021/acs.analchem.2c02720

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Accepteret manuskript, 0,99 MB
Embargo slutter: 27/12/2023

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Citationsformater

Andersen, C., Zulueta Díaz, Y. D. L. M., Lavrsen Kure, J., Eriksen, M. H., Lovatt, A. L., Lagerholm , C., Morales , S., Sehayek , S., Sheard , T. M. D., Wiseman , P. W., & Arnspang, E. C. (2023). Angiotensin II Treatment Induces Reorganization and Changes in the Lateral Dynamics of Angiotensin II Type 1 Receptor in the Plasma Membrane Elucidated by Photoactivated Localization Microscopy Combined with Image Spatial Correlation Analysis. Analytical Chemistry, 95(2), 730–738. https://doi.org/10.1021/acs.analchem.2c02720

Andersen, Camilla ; Zulueta Díaz, Yenisleidy de las Mercedes ; Lavrsen Kure, Jakob et al. / Angiotensin II Treatment Induces Reorganization and Changes in the Lateral Dynamics of Angiotensin II Type 1 Receptor in the Plasma Membrane Elucidated by Photoactivated Localization Microscopy Combined with Image Spatial Correlation Analysis. I: Analytical Chemistry. 2023 ; Bind 95, Nr. 2. s. 730–738.

@article{098b695cceb04288a543a592612a7767,

title = "Angiotensin II Treatment Induces Reorganization and Changes in the Lateral Dynamics of Angiotensin II Type 1 Receptor in the Plasma Membrane Elucidated by Photoactivated Localization Microscopy Combined with Image Spatial Correlation Analysis",

abstract = "The mechanisms by which angiotensin II type 1 receptor is distributed and the diffusional pattern in the plasma membrane (PM) remain unclear, despite their crucial role in cardiovascular homeostasis. In this work, we obtained quantitative information of angiotensin II type 1 receptor (AT1R) lateral dynamics as well as changes in the diffusion properties after stimulation with ligands in living cells using photoactivated localization microscopy (PALM) combined with image spatial-temporal correlation analysis. To study the organization of the receptor at the nanoscale, expansion microscopy (ExM) combined with PALM was performed. This study revealed that AT1R lateral diffusion increased after binding to angiotensin II (Ang II) and the receptor diffusion was transiently confined in the PM. In addition, ExM revealed that AT1R formed nanoclusters at the PM and the cluster size significantly decreased after Ang II treatment. Taking these results together suggest that Ang II binding and activation cause reorganization and changes in the dynamics of AT1R at the PM.",

keywords = "Super-resolution ﬂuorescence microscopy, AT1R, image spatial correlation analysis, Lateral dynamics, Receptor, Angiotensin, Type 1/metabolism, Microscopy, Cell Membrane/metabolism, Angiotensin II/pharmacology",

author = "Camilla Andersen and {Zulueta D{\'i}az}, {Yenisleidy de las Mercedes} and {Lavrsen Kure}, Jakob and Eriksen, {Mathias Hessellund} and Lovatt, {Adam Leslie} and Christoffer Lagerholm and Sebastian Morales and Simon Sehayek and Sheard, {Thomas M.D} and Wiseman, {Paul W.} and Arnspang, {Eva C.}",

year = "2023",

month = jan,

day = "17",

doi = "10.1021/acs.analchem.2c02720",

language = "English",

volume = "95",

pages = "730–738",

journal = "Analytical Chemistry",

issn = "0003-2700",

publisher = "American Chemical Society",

number = "2",

}

Andersen, C, Zulueta Díaz, YDLM, Lavrsen Kure, J, Eriksen, MH, Lovatt, AL, Lagerholm , C, Morales , S, Sehayek , S, Sheard , TMD, Wiseman , PW & Arnspang, EC 2023, 'Angiotensin II Treatment Induces Reorganization and Changes in the Lateral Dynamics of Angiotensin II Type 1 Receptor in the Plasma Membrane Elucidated by Photoactivated Localization Microscopy Combined with Image Spatial Correlation Analysis', Analytical Chemistry, bind 95, nr. 2, s. 730–738. https://doi.org/10.1021/acs.analchem.2c02720

Angiotensin II Treatment Induces Reorganization and Changes in the Lateral Dynamics of Angiotensin II Type 1 Receptor in the Plasma Membrane Elucidated by Photoactivated Localization Microscopy Combined with Image Spatial Correlation Analysis. / Andersen, Camilla; Zulueta Díaz, Yenisleidy de las Mercedes; Lavrsen Kure, Jakob et al.

I: Analytical Chemistry, Bind 95, Nr. 2, 17.01.2023, s. 730–738.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - Angiotensin II Treatment Induces Reorganization and Changes in the Lateral Dynamics of Angiotensin II Type 1 Receptor in the Plasma Membrane Elucidated by Photoactivated Localization Microscopy Combined with Image Spatial Correlation Analysis

AU - Andersen, Camilla

AU - Zulueta Díaz, Yenisleidy de las Mercedes

AU - Lavrsen Kure, Jakob

AU - Eriksen, Mathias Hessellund

AU - Lovatt, Adam Leslie

AU - Lagerholm , Christoffer

AU - Morales , Sebastian

AU - Sehayek , Simon

AU - Sheard , Thomas M.D

AU - Wiseman , Paul W.

AU - Arnspang, Eva C.

PY - 2023/1/17

Y1 - 2023/1/17

N2 - The mechanisms by which angiotensin II type 1 receptor is distributed and the diffusional pattern in the plasma membrane (PM) remain unclear, despite their crucial role in cardiovascular homeostasis. In this work, we obtained quantitative information of angiotensin II type 1 receptor (AT1R) lateral dynamics as well as changes in the diffusion properties after stimulation with ligands in living cells using photoactivated localization microscopy (PALM) combined with image spatial-temporal correlation analysis. To study the organization of the receptor at the nanoscale, expansion microscopy (ExM) combined with PALM was performed. This study revealed that AT1R lateral diffusion increased after binding to angiotensin II (Ang II) and the receptor diffusion was transiently confined in the PM. In addition, ExM revealed that AT1R formed nanoclusters at the PM and the cluster size significantly decreased after Ang II treatment. Taking these results together suggest that Ang II binding and activation cause reorganization and changes in the dynamics of AT1R at the PM.

AB - The mechanisms by which angiotensin II type 1 receptor is distributed and the diffusional pattern in the plasma membrane (PM) remain unclear, despite their crucial role in cardiovascular homeostasis. In this work, we obtained quantitative information of angiotensin II type 1 receptor (AT1R) lateral dynamics as well as changes in the diffusion properties after stimulation with ligands in living cells using photoactivated localization microscopy (PALM) combined with image spatial-temporal correlation analysis. To study the organization of the receptor at the nanoscale, expansion microscopy (ExM) combined with PALM was performed. This study revealed that AT1R lateral diffusion increased after binding to angiotensin II (Ang II) and the receptor diffusion was transiently confined in the PM. In addition, ExM revealed that AT1R formed nanoclusters at the PM and the cluster size significantly decreased after Ang II treatment. Taking these results together suggest that Ang II binding and activation cause reorganization and changes in the dynamics of AT1R at the PM.

KW - Super-resolution ﬂuorescence microscopy

KW - AT1R

KW - image spatial correlation analysis

KW - Lateral dynamics

KW - Receptor, Angiotensin, Type 1/metabolism

KW - Microscopy

KW - Cell Membrane/metabolism

KW - Angiotensin II/pharmacology

U2 - 10.1021/acs.analchem.2c02720

DO - 10.1021/acs.analchem.2c02720

M3 - Journal article

C2 - 36574961

VL - 95

SP - 730

EP - 738

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 2

ER -

Andersen C, Zulueta Díaz YDLM, Lavrsen Kure J, Eriksen MH, Lovatt AL, Lagerholm C et al. Angiotensin II Treatment Induces Reorganization and Changes in the Lateral Dynamics of Angiotensin II Type 1 Receptor in the Plasma Membrane Elucidated by Photoactivated Localization Microscopy Combined with Image Spatial Correlation Analysis. Analytical Chemistry. 2023 jan. 17;95(2):730–738. Epub 2022 dec. 27. doi: 10.1021/acs.analchem.2c02720