Analytical assessment of the osteoinductive material COLLOSSE

M E L Nienhuijs, K Poulsen, M van der Zande, A Briest, M A W Merkx, P J W Stoelinga, Ole Nørregaard Jensen, J A Jansen, X F Walboomers

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

In this study, the growth factors in COLLOSSE were analyzed, using ELISA tests, mass spectrometry, western blotting, and a 24-day cell culture experiment using osteoblast-like cells. The results of the ELISA testing, mass spectrometry, and western blotting all confirmed that TGF-beta1 was the main growth factor in COLLOSSE at 55 ng/mg. The results from the culture test showed that the cell proliferation, alkaline phosphatase activity, and matrix calcification were all drastically changed by the addition of COLLOSSE, mirroring the effects of addition of TGF-beta1. We conclude that COLLOSSE is not only a rich source of TGFbeta-1, but also contains the growth factors TGFbeta-2, BMP-2, BMP-3, BMP-7, IGF-1, and possibly VEGF. Other growth factors might be present in COLLOSSE, but were not identified due to inherent detection limits of the used ELISA and mass spectrometry techniques. The number of osteoinductive factors in COLLOSSE causes a synergistic effect, explaining the new bone formation found in previously described in vivo studies, with much lower growth factor concentrations when compared with recombinant BMPs.
OriginalsprogEngelsk
TidsskriftJournal of Biomedical Materials Research. Part B: Applied Biomaterials
Vol/bind89B
Udgave nummer2
Sider (fra-til)300-305
Antal sider5
ISSN1552-4973
DOI
StatusUdgivet - 2009

Fingeraftryk

Intercellular Signaling Peptides and Proteins
Mass spectrometry
Transforming Growth Factor beta1
Cell culture
Transforming Growth Factor beta
Bone Morphogenetic Protein 7
Osteoblasts
Phosphatases
Cell proliferation
Insulin-Like Growth Factor I
Vascular Endothelial Growth Factor A
Alkaline Phosphatase
Bone
Testing
Experiments

Citer dette

Nienhuijs, M. E. L., Poulsen, K., van der Zande, M., Briest, A., Merkx, M. A. W., Stoelinga, P. J. W., ... Walboomers, X. F. (2009). Analytical assessment of the osteoinductive material COLLOSSE. Journal of Biomedical Materials Research. Part B: Applied Biomaterials, 89B(2), 300-305. https://doi.org/10.1002/jbm.b.31216
Nienhuijs, M E L ; Poulsen, K ; van der Zande, M ; Briest, A ; Merkx, M A W ; Stoelinga, P J W ; Jensen, Ole Nørregaard ; Jansen, J A ; Walboomers, X F. / Analytical assessment of the osteoinductive material COLLOSSE. I: Journal of Biomedical Materials Research. Part B: Applied Biomaterials. 2009 ; Bind 89B, Nr. 2. s. 300-305.
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title = "Analytical assessment of the osteoinductive material COLLOSSE",
abstract = "In this study, the growth factors in COLLOSSE were analyzed, using ELISA tests, mass spectrometry, western blotting, and a 24-day cell culture experiment using osteoblast-like cells. The results of the ELISA testing, mass spectrometry, and western blotting all confirmed that TGF-beta1 was the main growth factor in COLLOSSE at 55 ng/mg. The results from the culture test showed that the cell proliferation, alkaline phosphatase activity, and matrix calcification were all drastically changed by the addition of COLLOSSE, mirroring the effects of addition of TGF-beta1. We conclude that COLLOSSE is not only a rich source of TGFbeta-1, but also contains the growth factors TGFbeta-2, BMP-2, BMP-3, BMP-7, IGF-1, and possibly VEGF. Other growth factors might be present in COLLOSSE, but were not identified due to inherent detection limits of the used ELISA and mass spectrometry techniques. The number of osteoinductive factors in COLLOSSE causes a synergistic effect, explaining the new bone formation found in previously described in vivo studies, with much lower growth factor concentrations when compared with recombinant BMPs.",
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Nienhuijs, MEL, Poulsen, K, van der Zande, M, Briest, A, Merkx, MAW, Stoelinga, PJW, Jensen, ON, Jansen, JA & Walboomers, XF 2009, 'Analytical assessment of the osteoinductive material COLLOSSE', Journal of Biomedical Materials Research. Part B: Applied Biomaterials, bind 89B, nr. 2, s. 300-305. https://doi.org/10.1002/jbm.b.31216

Analytical assessment of the osteoinductive material COLLOSSE. / Nienhuijs, M E L; Poulsen, K; van der Zande, M; Briest, A; Merkx, M A W; Stoelinga, P J W; Jensen, Ole Nørregaard; Jansen, J A; Walboomers, X F.

I: Journal of Biomedical Materials Research. Part B: Applied Biomaterials, Bind 89B, Nr. 2, 2009, s. 300-305.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Analytical assessment of the osteoinductive material COLLOSSE

AU - Nienhuijs, M E L

AU - Poulsen, K

AU - van der Zande, M

AU - Briest, A

AU - Merkx, M A W

AU - Stoelinga, P J W

AU - Jensen, Ole Nørregaard

AU - Jansen, J A

AU - Walboomers, X F

N1 - (c) 2008 Wiley Periodicals, Inc.

PY - 2009

Y1 - 2009

N2 - In this study, the growth factors in COLLOSSE were analyzed, using ELISA tests, mass spectrometry, western blotting, and a 24-day cell culture experiment using osteoblast-like cells. The results of the ELISA testing, mass spectrometry, and western blotting all confirmed that TGF-beta1 was the main growth factor in COLLOSSE at 55 ng/mg. The results from the culture test showed that the cell proliferation, alkaline phosphatase activity, and matrix calcification were all drastically changed by the addition of COLLOSSE, mirroring the effects of addition of TGF-beta1. We conclude that COLLOSSE is not only a rich source of TGFbeta-1, but also contains the growth factors TGFbeta-2, BMP-2, BMP-3, BMP-7, IGF-1, and possibly VEGF. Other growth factors might be present in COLLOSSE, but were not identified due to inherent detection limits of the used ELISA and mass spectrometry techniques. The number of osteoinductive factors in COLLOSSE causes a synergistic effect, explaining the new bone formation found in previously described in vivo studies, with much lower growth factor concentrations when compared with recombinant BMPs.

AB - In this study, the growth factors in COLLOSSE were analyzed, using ELISA tests, mass spectrometry, western blotting, and a 24-day cell culture experiment using osteoblast-like cells. The results of the ELISA testing, mass spectrometry, and western blotting all confirmed that TGF-beta1 was the main growth factor in COLLOSSE at 55 ng/mg. The results from the culture test showed that the cell proliferation, alkaline phosphatase activity, and matrix calcification were all drastically changed by the addition of COLLOSSE, mirroring the effects of addition of TGF-beta1. We conclude that COLLOSSE is not only a rich source of TGFbeta-1, but also contains the growth factors TGFbeta-2, BMP-2, BMP-3, BMP-7, IGF-1, and possibly VEGF. Other growth factors might be present in COLLOSSE, but were not identified due to inherent detection limits of the used ELISA and mass spectrometry techniques. The number of osteoinductive factors in COLLOSSE causes a synergistic effect, explaining the new bone formation found in previously described in vivo studies, with much lower growth factor concentrations when compared with recombinant BMPs.

KW - Animals

KW - Biocompatible Materials

KW - Bone Morphogenetic Proteins

KW - Cell Proliferation

KW - Cells, Cultured

KW - Collagen

KW - Growth Substances

KW - Guided Tissue Regeneration

KW - Humans

KW - Male

KW - Materials Testing

KW - Osteoblasts

KW - Osteogenesis

KW - Rats

KW - Rats, Wistar

KW - Transforming Growth Factor beta1

U2 - 10.1002/jbm.b.31216

DO - 10.1002/jbm.b.31216

M3 - Journal article

VL - 89B

SP - 300

EP - 305

JO - Journal of Biomedical Materials Research. Part B: Applied Biomaterials

JF - Journal of Biomedical Materials Research. Part B: Applied Biomaterials

SN - 1552-4973

IS - 2

ER -