Analysis of Secreted Proteins Using SILAC

Jeanette Henningsen, Blagoy Blagoev, Irina Kratchmarova

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Secreted proteins serve a crucial role in the communication between cells, tissues, and organs. Proteins released to the extracellular environment exert their function either locally or at distant points of the organism. Proteins are secreted in a highly dynamic fashion by cells and tissues in the body responding to the stimuli and requirements presented by the extracellular milieu. Characterization of secretomes derived from various cell types has been performed using different quantitative mass spectrometry-based proteomics strategies, several of them taking advantage of labeling with stable isotopes. Here, we describe the use of Stable Isotope Labeling by Amino acids in Cell culture (SILAC) for the quantitative analysis of the skeletal muscle secretome during myogenesis.

OriginalsprogEngelsk
TidsskriftMethods in Molecular Biology
Vol/bind1188
Sider (fra-til)313-26
Antal sider14
ISSN1064-3745
DOI
StatusUdgivet - 2014

Fingeraftryk

Isotope Labeling
Proteins
Muscle Development
Cell Communication
Skeletal Muscle
Amino Acids

Citer dette

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Analysis of Secreted Proteins Using SILAC. / Henningsen, Jeanette; Blagoev, Blagoy; Kratchmarova, Irina.

I: Methods in Molecular Biology, Bind 1188, 2014, s. 313-26.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

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