Analysis of quality raw data of second generation sequencers with Quality Assessment Software

Rommel Tj Ramos, Adriana R Carneiro, Jan Baumbach, Vasco Azevedo, Maria Pc Schneider, Artur Silva

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Second generation technologies have advantages over Sanger; however, they have resulted in new challenges for the genome construction process, especially because of the small size of the reads, despite the high degree of coverage. Independent of the program chosen for the construction process, DNA sequences are superimposed, based on identity, to extend the reads, generating contigs; mismatches indicate a lack of homology and are not included. This process improves our confidence in the sequences that are generated.
OriginalsprogEngelsk
TidsskriftBMC Research Notes
Vol/bind4
Sider (fra-til)130
ISSN1756-0500
DOI
StatusUdgivet - 2011
Udgivet eksterntJa

Fingeraftryk

DNA sequences
Genes
Data Accuracy

Citer dette

Ramos, Rommel Tj ; Carneiro, Adriana R ; Baumbach, Jan ; Azevedo, Vasco ; Schneider, Maria Pc ; Silva, Artur. / Analysis of quality raw data of second generation sequencers with Quality Assessment Software. I: BMC Research Notes. 2011 ; Bind 4. s. 130.
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Analysis of quality raw data of second generation sequencers with Quality Assessment Software. / Ramos, Rommel Tj; Carneiro, Adriana R; Baumbach, Jan; Azevedo, Vasco; Schneider, Maria Pc; Silva, Artur.

I: BMC Research Notes, Bind 4, 2011, s. 130.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

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AU - Schneider, Maria Pc

AU - Silva, Artur

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