An improved method of sample preparation on AnchorChip targets for MALDI-MS and MS/MS and its application in the liver proteome project

Xumin Zhang, Liang Shi, Shaokung Shu, Yuan Wang, Kang Zhao, Ningzhi Xu, Siqi Liu, Peter Roepstorff

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

 
Udgivelsesdato: 2007-Jul
OriginalsprogEngelsk
TidsskriftProteomics
Vol/bind7
Udgave nummer14
Sider (fra-til)2340-2349
Antal sider9
ISSN1615-9853
DOI
StatusUdgivet - 1. jul. 2007

Fingeraftryk

Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Proteome
Liver
Salt removal
Tryptophan
Methionine
Proteins
Automation
Oxidation
Peptides

Citer dette

Zhang, Xumin ; Shi, Liang ; Shu, Shaokung ; Wang, Yuan ; Zhao, Kang ; Xu, Ningzhi ; Liu, Siqi ; Roepstorff, Peter. / An improved method of sample preparation on AnchorChip targets for MALDI-MS and MS/MS and its application in the liver proteome project. I: Proteomics. 2007 ; Bind 7, Nr. 14. s. 2340-2349.
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title = "An improved method of sample preparation on AnchorChip targets for MALDI-MS and MS/MS and its application in the liver proteome project",
abstract = "An improved method for sample preparation for MALDI-MS and MS/MS using AnchorChip targets is presented. The method, termed the SMW method (sample, matrix wash), results in better sensitivity for peptide mass fingerprinting as well as for sequencing by MS/MS than previously published methods. The method allows up-concentration and desalting directly on the mass spectrometric target and should be amenable for automation. A draw back caused by extensive oxidation of methionine and tryptophan in the SMW method can be alleviated by the addition of n-octyl glucopyranoside and DTT to the sample solution. The method was validated for protein identification from a 2-DE based liver proteome study. The SMW method resulted in identification of many more proteins and in most cases with a better score than the previously published methods.",
keywords = "Animals, Crystallization, Electrophoresis, Gel, Two-Dimensional, Liver, Male, Mice, Microchip Analytical Procedures, Oxidation-Reduction, Proteome, Proteomics, Reproducibility of Results, Sensitivity and Specificity, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Tandem Mass Spectrometry",
author = "Xumin Zhang and Liang Shi and Shaokung Shu and Yuan Wang and Kang Zhao and Ningzhi Xu and Siqi Liu and Peter Roepstorff",
year = "2007",
month = "7",
day = "1",
doi = "10.1002/pmic.200600184",
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An improved method of sample preparation on AnchorChip targets for MALDI-MS and MS/MS and its application in the liver proteome project. / Zhang, Xumin; Shi, Liang; Shu, Shaokung; Wang, Yuan; Zhao, Kang; Xu, Ningzhi; Liu, Siqi; Roepstorff, Peter.

I: Proteomics, Bind 7, Nr. 14, 01.07.2007, s. 2340-2349.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - An improved method of sample preparation on AnchorChip targets for MALDI-MS and MS/MS and its application in the liver proteome project

AU - Zhang, Xumin

AU - Shi, Liang

AU - Shu, Shaokung

AU - Wang, Yuan

AU - Zhao, Kang

AU - Xu, Ningzhi

AU - Liu, Siqi

AU - Roepstorff, Peter

PY - 2007/7/1

Y1 - 2007/7/1

N2 - An improved method for sample preparation for MALDI-MS and MS/MS using AnchorChip targets is presented. The method, termed the SMW method (sample, matrix wash), results in better sensitivity for peptide mass fingerprinting as well as for sequencing by MS/MS than previously published methods. The method allows up-concentration and desalting directly on the mass spectrometric target and should be amenable for automation. A draw back caused by extensive oxidation of methionine and tryptophan in the SMW method can be alleviated by the addition of n-octyl glucopyranoside and DTT to the sample solution. The method was validated for protein identification from a 2-DE based liver proteome study. The SMW method resulted in identification of many more proteins and in most cases with a better score than the previously published methods.

AB - An improved method for sample preparation for MALDI-MS and MS/MS using AnchorChip targets is presented. The method, termed the SMW method (sample, matrix wash), results in better sensitivity for peptide mass fingerprinting as well as for sequencing by MS/MS than previously published methods. The method allows up-concentration and desalting directly on the mass spectrometric target and should be amenable for automation. A draw back caused by extensive oxidation of methionine and tryptophan in the SMW method can be alleviated by the addition of n-octyl glucopyranoside and DTT to the sample solution. The method was validated for protein identification from a 2-DE based liver proteome study. The SMW method resulted in identification of many more proteins and in most cases with a better score than the previously published methods.

KW - Animals

KW - Crystallization

KW - Electrophoresis, Gel, Two-Dimensional

KW - Liver

KW - Male

KW - Mice

KW - Microchip Analytical Procedures

KW - Oxidation-Reduction

KW - Proteome

KW - Proteomics

KW - Reproducibility of Results

KW - Sensitivity and Specificity

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

KW - Tandem Mass Spectrometry

U2 - 10.1002/pmic.200600184

DO - 10.1002/pmic.200600184

M3 - Journal article

VL - 7

SP - 2340

EP - 2349

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 14

ER -