An Aptamer-Based Nanobiosensor for Real-Time Measurements of ATP Dynamics

Cengiz Özalp; Lise Junker Nielsen; Lars Folke Olsen

doi:10.1002/cbic.201000500

An Aptamer-Based Nanobiosensor for Real-Time Measurements of ATP Dynamics

Cengiz Özalp, Lise Junker Nielsen, Lars Folke Olsen

Rovira i Virgili University

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Abstract

A nanosensor based on a new DNA aptamer can show changes in intracellular concentration of ATP of 0.5 to 8 mM and thereby the kinetics of ATP-consuming reactions in real-time. The biosensor was protected against nuclease attack by being buried in a polyacrylamide nanoparticle. This strategy can readily be applied to any metabolite of interest in cells.

Originalsprog	Engelsk
Tidsskrift	ChemBioChem
Vol/bind	11
Sider (fra-til)	2538-2541
Antal sider	4
ISSN	1439-7633
DOI	https://doi.org/10.1002/cbic.201000500
Status	Udgivet - 2010

Dokumenter og links

10.1002/cbic.201000500

Citationsformater

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abstract = "A nanosensor based on a new DNA aptamer can show changes in intracellular concentration of ATP of 0.5 to 8 mM and thereby the kinetics of ATP-consuming reactions in real-time. The biosensor was protected against nuclease attack by being buried in a polyacrylamide nanoparticle. This strategy can readily be applied to any metabolite of interest in cells.",

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AU - Olsen, Lars Folke

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N2 - A nanosensor based on a new DNA aptamer can show changes in intracellular concentration of ATP of 0.5 to 8 mM and thereby the kinetics of ATP-consuming reactions in real-time. The biosensor was protected against nuclease attack by being buried in a polyacrylamide nanoparticle. This strategy can readily be applied to any metabolite of interest in cells.

AB - A nanosensor based on a new DNA aptamer can show changes in intracellular concentration of ATP of 0.5 to 8 mM and thereby the kinetics of ATP-consuming reactions in real-time. The biosensor was protected against nuclease attack by being buried in a polyacrylamide nanoparticle. This strategy can readily be applied to any metabolite of interest in cells.

U2 - 10.1002/cbic.201000500

DO - 10.1002/cbic.201000500

M3 - Journal article

C2 - 21086483

SN - 1439-7633

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JO - ChemBioChem

JF - ChemBioChem

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